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PIM1 antibody

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Principal name

PIM1 antibody

Alternative names for PIM1 antibody

Pim-1, Proto-oncogene serine/threonine-protein kinase pim-1

SwissProt ID

P06803 (Mouse), P11309 (Human), P26794 (Rat), Q95LJ0 (Felca), Q9N0P9 (Bovin), Q9YHZ5 (Danre)

Gene ID

5292 (PIM1)

Ncbi ID

9606, NP_002639.1, NP_002639, NM_002648, 10090

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Sh (Sheep)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of PIM1 antibody

The protein encoded by this gene belongs to the Ser/Thr protein kise family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic maligncies and in prostate cancer. It plays a role in sigl transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of altertive in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011].

General readings

Koike N., et al. (2000) FEBS Lett. 467:17-21Ionov Y., et al. (2003) Anticancer Res. 23:167-178

H00005292-M01 - PIM1
Detection limit for recombinant GST tagged PIM1 is approximately 0.1ng/ml as a capture antibody.
H00005292-M05 - PIM1
Immunoperoxidase of monoclonal antibody to PIM1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
H00005292-M08 - PIM1
Detection limit for recombinant GST tagged PIM1 is approximately 0.1ng/ml as a capture antibody.
H00005292-M10 - PIM1
Immunoperoxidase of monoclonal antibody to PIM1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 1.5 ug/ml]
H00005292-M16 - PIM1
Immunoperoxidase of monoclonal antibody to PIM1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 1 ug/ml]
H00005292-M17 - PIM1
Detection limit for recombinant GST tagged PIM1 is approximately 0.3ng/ml as a capture antibody.
PAB2304 - PIM1
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the PIM1 polyclonal antibody ( Cat # PAB2304 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
PAB2304 - PIM1
Western blot analysis of PIM1 polyclonal antibody ( Cat # PAB2304 ) in A-549 cell lysate. PIM1 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
AP01350PU-N - PIM1
Western blot (WB) analysis of Pim-1 antibody (Cat.-No.: AP01350PU-N) in extracts from Jurkat cells.
AP14812PU-N - PIM1
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PIM1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP14812PU-N - PIM1
Western blot analysis of PIM1 (arrow) using PIM1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIM1 gene (Lane 2) (Origene Technologies).
ARP56389_P050 - PIM1
Hum. Fetal Lung; Host: Rabbit . Target Name: PIM1 . Sample Tissue: Human Fetal Lung . Antibody Dilution: 1.0ug/ml.; PIM1 antibody - N-terminal region (ARP56389_P050) in Hum. Fetal Lung cells using Western Blot
ARP56389_P050 - PIM1
Human Heart; WB Suggested Anti-PIM1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human heart; PIM1 antibody - N-terminal region (ARP56389_P050) in Human Heart cells using Western Blot
ARP56390_P050 - PIM1
Human 293T; WB Suggested Anti-PIM1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. PIM1 is supported by BioGPS gene expression data to be expressed in HEK293T.; PIM1 antibody - N-terminal region (ARP56390_P050) in Human 293T cells using Western Blot
A0712-1 - PIM1
Western blot analysis of extracts from HuvEc cells treated with PMA 125ng/ml 30', using Pim-1 (Phospho-Tyr309) Antibody. The lane on the right is treated with the synthesized peptide.
B0712-1 - PIM1
Western blot analysis of extracts from Jurkat cells, using Pim-1 (Ab-309) Antibody. The lane on the right is treated with the synthesized peptide.
AP23807PU-M - PIM1
AP23807PU PIM1 antibody staining of Human Bone Marrow.
AM31121PU-N - PIM1
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31122PU-N - PIM1
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31122PU-N - PIM1
Immunofluorescence of monoclonal antibody to PIM1 on HeLa cell . [antibody concentration 10 µg/ml]
AM31123PU-N - PIM1
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31123PU-N - PIM1
Immunofluorescence of monoclonal antibody to PIM1 on HeLa cell . [antibody concentration 10 µg/ml]
AM31764PU-N - PIM1
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31764PU-N - PIM1
Immunofluorescence of monoclonal antibody to PIM1 on HeLa cell . [antibody concentration 10 µg/ml]
AR50821PU-N - PIM1
AP55710PU-S - PIM1
Western blot analysis of extracts from HUVEC cells treated with PMA using Pim-1 (Phospho-Tyr309) Antibody AP55710PU-N.The lane on the right is treated with the antigen-specific peptide.
TP305853 - PIM1
TA300039 - PIM1
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
TA300039 - PIM1
(LEFT)Western blot analysis of anti-PIM1 Pab in A549 cell lysate. PIM1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of PIM1 (arrow) using PIM1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIM1 gene (Lane 2) (Origene Technologies).
TA318550 - PIM1
Anti-PIM1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
TA318959 - PIM1
Western blot analysis of PIM1 using Jurkat cell lysate.
TA337681 - PIM1
WB Suggested Anti-PIM1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human heart
TA337681 - PIM1
Host: Rabbit; Target Name: PIM1; Sample Tissue: Human Fetal Lung; Antibody Dilution: 1.0ug/ml
TA337682 - PIM1
WB Suggested Anti-PIM1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysatePIM1 is supported by BioGPS gene expression data to be expressed in HEK293T
TA347806 - PIM1
Western blot analysis on HuvEc cell lysate using Pim-1 Antibody

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PIM1 (C-term) antibody
  PIM1 Antibody (C-term ) Rabbit Polyclonal Antibody  
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €410.00
  OriGene Technologies, Inc.
+1 additional image

PIM1 antibody
  Rabbit polyclonal anti-PIM1 antibody  
Western blot analysis of PIM1 using Jurkat cell lysate. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €353.00
  OriGene Technologies, Inc.

PIM1 antibody
  Rabbit Polyclonal Anti-PIM1 Antibody  
WB Suggested Anti-PIM1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €360.00
  OriGene Technologies, Inc.
+1 additional image

PIM1 antibody
  Rabbit Polyclonal Anti-PIM1 Antibody  
WB Suggested Anti-PIM1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysatePIM1 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €360.00
  OriGene Technologies, Inc.

PIM1 antibody
  Rabbit Polyclonal Anti-Pim-1 Antibody  
Western blot analysis on HuvEc cell lysate using Pim-1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

PIM1 antibody
  Rabbit Polyclonal Anti-PIM1 Antibody  
PIM1 Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

PIM1 antibody
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2C9 Purified Hu E, P, WB
50 µg / €420.00
  Acris Antibodies GmbH

PIM1 antibody
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 6A2 Purified Hu E, ICC/IF, P
50 µg / €420.00
  Acris Antibodies GmbH
+1 additional image

PIM1 antibody
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3F5 Purified Hu E, ICC/IF, P
50 µg / €420.00
  Acris Antibodies GmbH
+1 additional image

PIM1 antibody
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a X1 Purified Hu E, ICC/IF, P
50 µg / €420.00
  Acris Antibodies GmbH
+1 additional image

PIM1 antibody
Western blot (WB) analysis of Pim-1 antibody (Cat.-No.: AP01350PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PIM1 antibody
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PIM1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH
+1 additional image

PIM1 pTyr309 antibody
Western blot analysis of extracts from HUVEC cells treated with PMA using Pim-1 (Phospho-Tyr309) Antibody AP55710PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

PIM1 pTyr309 antibody
Western blot analysis of extracts from HUVEC cells treated with PMA using Pim-1 (Phospho-Tyr309) Antibody AP55710PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €270.00
  Acris Antibodies GmbH

PIM1 pTyr309 antibody
Western blot analysis of extracts from HuvEc cells treated with PMA 125ng/ml 30', using Pim-1 (Phospho-Tyr309) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

PIM1 pTyr309 antibody
Western blot analysis of extracts from HuvEc cells treated with PMA 125ng/ml 30', using Pim-1 (Phospho-Tyr309) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

PIM1 antibody
Western blot analysis of extracts from Jurkat cells, using Pim-1 (Ab-309) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

PIM1 antibody
Western blot analysis of extracts from Jurkat cells, using Pim-1 (Ab-309) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PIM1
  Recombinant protein of human pim-1 oncogene (PIM1)  
PIM1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.
 

PIM1 (38-290, His-tag)
PIM1 Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

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21 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Primary Antibody (18)
  2. Protein | Growth Factor (3)
Clonality
  1. Polyclonal (14)
  2. Monoclonal (4)
Target
  1. PIM1 (21)
Property
  1. C-term (1)
  2. pTyr309 (4)
Reactivity
  1. Human (Hu) (18)
  2. Mouse (Ms) (12)
  3. Rat (Rt) (12)
  4. Bovine (Bov) (2)
  5. Canine (Can) (2)
  6. Equine (Eq) (2)
  7. Guinea Pig (GP) (2)
  8. Porcine (Por) (2)
  9. Rabbit (Rb) (2)
  10. Sheep (Sh) (2)
Applications
  1. Western blot / Immunoblot (WB) (14)
  2. Enzyme Immunoassay (E) (9)
  3. Paraffin Sections (P) (7)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
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  2. Aff - Purified (7)
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  2. Mouse (4)
Images
  1. 1 image (20)
Images: Applications
  1. Western blot / Immunoblot (WB) (13)
  2. Paraffin Sections (P) (6)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  4. SDS-PAGE (2)
Species (Protein)
  1. Human (3)
Source
  1. E. coli (2)
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