PIN1 antibody

Principal name

PIN1 antibody

Alternative names for PIN1 antibody

PIN-1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1

SwissProt ID

O74448 (Schpo), Q13526 (Human), Q5BIN5 (Bovin), Q9QUR7 (Mouse), Q9SL42 (Arath)

Gene ID

5300 (PIN1), 23988 (Pin1)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey), Bac (Bacteria), Por (Porcine), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)

Background of PIN1 antibody

The peptidyl prolyl cis/trans isomerase Ess1/Pin1 is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. PIN1-deficinet mice though underwent normal development, the embryonic PIN1 lacking fibroblast exhibited significant deficiency in their ability to restart proliferation in response to serum stimulation after a G(0) arrest suggesting that PIN1 is required for cell cycle progression from G(0) arrest and in mitosis progression in normal mammalian cells (1). Pin 1interacts with SIL, an early transcription factor essential for embryonic development in T-cell leukemia-associated translocations and hyper phosphorylate it in a cell cycle–dependent manner (2). By virtue of its activity, PIN1 presumably interacts with NIMA and attenuate its mitosis-promoting activity. The PIN1 displays a preference for a acidic residue N-terminal to the isomerization of proline bond when proline is flanked by serine and threonine catalyzing p-Ser/P-Thr-Prol cis/trans isomerization. The interaction of PIN1 with tau protein in brain implicated its protective in neurodegenerative diseases such as Alzhiemers and frontotemporal dementia with Parkinsonism linked to chromosome 17. PIN1 binds and isomerizes tau and has a protective effect in tau stability (3).
PIN1 also functions as a positive regulator of neuronal death through a c-Jun-dependent mechanism. The ectopic PIN1 promoted caspase-dependent apoptosis of neurotrophic growth factor (NGF) maintained neurons. The PIN1 knockout condition accumulated phosphorylated c-Jun, inhibited the release of cytochrome c and delayed the cell death suggest a novel mechanism by which PIN1 promotes apoptosis involving activation of c-Jun (4). PIN1 also interacts with proto oncogene c-Myb thereby increasing the c-Myb trans-activation activity providing a new paradigm by which post-translational modifications phosphorylation controls c-Myb activity (5).
PIN1 is a 150-165 amino acid (approximately 20kDa) protein, the rat/mouse from is smaller, several splice variants are reported in the gene bank.

General readings

Pastorino L, Sun A, Lu PJ, Zhou XZ, Balastik M, Finn G, Wulf G, Lim J, Li SH, Li X, Xia W, Nicholson LK, Lu KP. The prolyl isomerase Pin1 regulates amyloid precursor protein processing and amyloid-beta production.Nature. 2006 Mar 23;440(7083):528-34. PMID: 16554819

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PIN1 antibody

Western blot analysis of PIN1 Antibody (Center) (Cat. #TA325085) in HL-60 cell line lysates (35ug/lane). PIN1 (arrow) was detected using the purified Pab. Rabbit IgG Purified Hu, Mky F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

PIN1 antibody

Western blot analysis of extracts of various cell lines, using PIN1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIN1 antibody

TA302573 (0.2µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIN1 antibody

Predicted band size: 18 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIN1 antibody

PIN1 Antibody(Cat. #TA324966) western blot analysis in 293,rat PC-12 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the PIN1 antibody detected the PIN1 protein (arrow). Mouse IgG1 855CT1.7.5 Ascites Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIN1 antibody

Western blot analysis of Pin1 expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIN1 (incl. pos. control) antibody

Detection of endogenous PIN1 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab PIN1 8C10 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2 Mouse IgG1 8C10 Purified Can, Hu, Ms E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

PIN1 antibody

Western blot using affinity purified anti-Pin1 antibody to detect endogenous Pin1 in HeLa whole cell lysates. The sample was run in duplicate. A band representing Pin1 is indicated by the arrowhead. Cell lysates were electrophoresed using a straight 15%  polyacrylamide gel, followed by transfer to nitrocellulose. The membrane was probed with the primary antibody at a 1:700 dilution. A 1:5,000 dilution of HRP Gt-a-Rabbit IgG was used with a 15 sec exposure time. Rabbit IgG Aff - Purified Bov, Can, Hu, Mky, Ms, Rt E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

PIN1 Control Peptide

PIN1
0.1 mg / €250.00
  Acris Antibodies GmbH

PIN1 (53-73) antibody

PIN1 Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PIN1 Control Peptide

PIN1
0.1 mg / €250.00
  Acris Antibodies GmbH

PIN1 (134-150) antibody

PIN1 Rabbit IgG Aff - Purified Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PIN1 antibody

PIN1 Chicken Ig Liquid Hu, Ms, Rt ICC/IF, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

PIN1 antibody

AP20127SU-N PIN1 antibody staining of HeLa cells at 1/1,000 dilution (Green). Fibrillarin antibody (Red). Pin-1 stains the nuclear matrix and, much more faintly, the cytoplasm. The Fibrillarin antibody marks nucleoli. Chicken Ig Serum Hu ICC/IF, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

PIN1 (N-term) antibody

Immunohistochemical staining of Formalin-fixed, Paraffin-embedded human brain (cerebellum) using anti-PIN1 antibody cat. no. AP39025PU-N Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P
50 µg / €470.00
  Acris Antibodies GmbH

PIN1 pSer16 antibody

Western blot analysis of extracts from COS7 cells treated with Insulin using Phospho-Pin1 (Ser16) antibody AP55912PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

PIN1 pSer16 antibody

Western blot analysis of extracts from COS7 cells treated with Insulin using Phospho-Pin1 (Ser16) antibody AP55912PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

PIN1 antibody

Flow cytometry analysis of Pin1 in Jurkat cell line staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate used. Isotype control antibody was mouse IgG control (black line). Mouse IgG1 3G8 Purified Hu E, F, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

PIN1 antibody

Flow cytometry analysis of Pin1 in Jurkat cell line staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate used. Isotype control antibody was mouse IgG control (black line). Mouse IgG1 3G8 Purified Hu E, F, WB
50 µl / €230.00
  Acris Antibodies GmbH

PIN1 antibody

PIN1 monoclonal antibody (M01), clone 2F2 Western Blot analysis of PIN1 expression in Jurkat ( Cat # L017V1 ). Mouse IgG2b 2F2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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