PIWIL1 antibody

Principal name

PIWIL1 antibody

Alternative names for PIWIL1 antibody

Piwi-like protein 1, HIWI

SwissProt ID

A6N7Y9 (Chick), Q8UVX0 (Danre), Q96J94 (Human), Q9JMB7 (Mouse)

Gene ID

9271 (PIWIL1), 57749 (Piwil1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Ze (Zebrafish), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of PIWIL1 antibody

PIWIL1 (UniProtKB/Swiss-Prot entry Q96J94) plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. PIWIL1 acts via the piR metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRs and Piwi proteins and govern the methylation and subsequent repression of transposons. It directly binds methylated piRs, a class of 24 to 30 nucleotide Rs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRs are probably involved in other processes during meiosis such as translation regulation. PIWIL1 probably is a component of some RISC complex, which mediates R cleavage and translatiol silencing. It also plays a role in the formation of chromatoid bodies and is required for some miRs stability.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PIWIL1 antibody

  Rabbit anti-PIWIL1 polyclonal antibody  
Western blot analysis of fetal and rattus testis lysates with anti-PIWIL1 antibody (1:500). Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

  Goat Anti-PIWI / HIWI Antibody  
(4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Goat Aff - Purified Can, Hu, Ms, Por, Rt E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIWIL1 (N-term) antibody

  Rabbit Polyclonal PIWI-L1 Antibody  
Western blot analysis of PIWI-L1 in HepG2 cell lysate with PIWI-L1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

  Anti-PIWIL1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human esophagus and colon cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using PIWIL1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

  Anti-PIWIL1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PIWIL1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

  MIWI Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using MIWI antibody. Rabbit IgG Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

  Rabbit Polyclonal Anti-PIWIL1 Antibody  
WB Suggested Anti-PIWIL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Stomach Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

  Rabbit Polyclonal Anti-PIWIL1 Antibody  
WB Suggested Anti-PIWIL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: CCRF-CEM cell lysatePIWIL1 is supported by BioGPS gene expression data to be expressed in CCRT-CEM Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

  Rabbit Polyclonal PIWIL1 Antibody  
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against PIWIL1. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:23,800. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

PIWIL1 - human gene knockout kit via CRISPR

PIWIL1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PIWIL1 - human gene knockout kit via CRISPR

PIWIL1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PIWIL1 - human gene knockout kit via CRISPR

PIWIL1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PIWIL1 - human gene knockout kit via CRISPR

PIWIL1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PIWIL1 (mGFP-tagged) - Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PIWIL1 (mGFP-tagged) - Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PIWIL1 (mGFP-tagged)-Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, PIWIL1 (mGFP-tagged)-Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,770.00
  OriGene Technologies, Inc.

Lenti ORF particles, PIWIL1 (Myc-DDK tagged) - Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PIWIL1 (Myc-DDK tagged) - Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Lenti ORF particles, PIWIL1 (Myc-DDK-tagged)-Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, PIWIL1 (Myc-DDK-tagged)-Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,590.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Piwil1 (GFP-tagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Piwil1 (GFP-tagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1), 200 uL, >10^7 TU/mL  
200 µl / €1,600.00
  OriGene Technologies, Inc.

Lenti ORF particles, Piwil1 (Myc-DDK-tagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Piwil1 (Myc-DDK-tagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1), 200 uL, >10^7 TU/mL  
200 µl / €1,440.00
  OriGene Technologies, Inc.

19 Item(s)

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