PIWIL1 antibody

Principal name

PIWIL1 antibody

Alternative names for PIWIL1 antibody

Piwi-like protein 1, HIWI

SwissProt ID

A6N7Y9 (Chick), Q8UVX0 (Danre), Q96J94 (Human), Q9JMB7 (Mouse)

Gene ID

9271 (PIWIL1), 57749 (Piwil1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Ze (Zebrafish), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of PIWIL1 antibody

PIWIL1 (UniProtKB/Swiss-Prot entry Q96J94) plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. PIWIL1 acts via the piR metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRs and Piwi proteins and govern the methylation and subsequent repression of transposons. It directly binds methylated piRs, a class of 24 to 30 nucleotide Rs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRs are probably involved in other processes during meiosis such as translation regulation. PIWIL1 probably is a component of some RISC complex, which mediates R cleavage and translatiol silencing. It also plays a role in the formation of chromatoid bodies and is required for some miRs stability.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PIWIL1 antibody

Western blot analysis of fetal and rattus testis lysates with anti-PIWIL1 antibody (1:500). Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

(4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Goat Aff - Purified Can, Hu, Ms, Por, Rt E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIWIL1 (N-term) antibody

Western blot analysis of PIWI-L1 in HepG2 cell lysate with PIWI-L1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

Predicted cell location: Cytoplasm. Positive control: Human esophagus and colon cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using PIWIL1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PIWIL1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

Western blot analysis of extracts of various cell lines, using MIWI antibody. Rabbit IgG Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

WB Suggested Anti-PIWIL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Stomach Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

WB Suggested Anti-PIWIL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: CCRF-CEM cell lysatePIWIL1 is supported by BioGPS gene expression data to be expressed in CCRT-CEM Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIWIL1 antibody

Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against PIWIL1. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:23,800. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIWIL1 control peptide

Western blot analysis of PIWI-L1 in HepG2 cell lysate with PIWI-L1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
50 µg / €160.00
  Acris Antibodies GmbH

PIWIL1 antibody

Western Blot analysis of PIWIL1 expression in transfected 293T cell line (H00009271-T01) by PIWIL1 MaxPab polyclonal antibody.

Lane 1: PIWIL1 transfected lysate(94.71 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PIWIL1 antibody

PIWIL1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

PIWIL1 antibody

PIWIL1 Goat Hu, Ms, Rt E
0.1 mg / €400.00
  Abnova Taiwan Corp.

PIWIL1 antibody

Human Stomach; WB Suggested Anti-PIWIL1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Stomach; PIWIL1 antibody - N-terminal region (ARP41339_P050) in Human Stomach cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

PIWIL1 antibody

Human CCRF-CEM; WB Suggested Anti-PIWIL1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: CCRF-CEM cell lysate. PIWIL1 is supported by BioGPS gene expression data to be expressed in CCRT-CEM.; PIWIL1 antibody - middle region (ARP41340_P050) in Human CCRF-CEM cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PIWIL1 antibody

PIWIL1 Goat IgG Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PIWIL1 antibody

Western Blot: PIWIL1 Antibody [NBP1-54796] - This Anti-PIWIL1 antibody was used in Western Blot of CCRF-CEM tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

PIWIL1 antibody

Western Blot: PIWIL1 Antibody [NBP1-55299] - This Anti-PIWIL1 antibody was used in Western Blot of Fetal Stomach   tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PIWIL1

PIWIL1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

PIWIL1

PIWIL1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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