PIWIL2 antibody

Principal name

PIWIL2 antibody

Alternative names for PIWIL2 antibody

Piwi-like protein 2, PIWIL1L, HILI, Hiwi-like, Mili, Miwi-like, CT80, Cancer/testis antigen 80

SwissProt ID

A2CEI6 (Danre), Q8CDG1 (Mouse), Q8TC59 (Human)

Gene ID

55124 (PIWIL2), 5551242 (BAA-894), 57746 (Piwil2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PIWIL2 antibody

PIWIL2 (UniProtKB/Swiss-Prot entry Q8TC59) plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. It plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. PIWL2 acts via the piR metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRs and Piwi proteins and governs the methylation and subsequent repression of transposons. It directly binds piRs, a class of 24 to 30 nucleotide Rs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. PIWIL2 indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Repression of PIWIL2 inhibits tumor cell growth.

General readings

Kuramochi-Miyagawa S, Kimura T, Yomogida K, et al. Two mouse piwi-related genes: miwi and mili. Mech. Dev. 108:121-33.

16 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PIWIL2 antibody

Western blot analysis of fetal testis lysates with anti-PIWIL2 antibody (1:400). Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIWIL2 (Center) antibody

Western blot analysis of PIWI-L2 in HepG2 cell lysate with PIWI-L1 antibody at 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIWIL2 (Center) antibody

Immunohistochemistry of PIWI-L2 in human brain tissue with PIWI-L2 antibody at 5 ug/mL. Rabbit IgG Aff - Purified Hu E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIWIL2 antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PIWIL2 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIWIL2 antibody

Predicted band size: 110 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIWIL2 antibody

WB Suggested Anti-PIWIL2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Small Intestine Rabbit IgG Purified Bov, Can, Eq, GP, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIWIL2 antibody

Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against PIWIL2. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,750. Rabbit IgG Purified Hu, Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIWIL2 (N-term) Control Peptide

PIWIL2
50 µg / €160.00
  Acris Antibodies GmbH

PIWIL2 (Center) Control Peptide

PIWIL2
50 µg / €160.00
  Acris Antibodies GmbH

PIWIL2 antibody

PIWIL2 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PIWIL2 antibody

PIWIL2 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

PIWIL2 antibody

Immunoblot analysis of mouse testis tissue lysate. Mouse IgG1 11C11 Purified Hu E, IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

PIWIL2 antibody

Human Small Intestine; WB Suggested Anti-PIWIL2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Small Intestine; PIWIL2 Antibody (ARP57108_P050) in Human Small Intestine cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PIWIL2 antibody

Western Blot: PIWIL2 Antibody [NBP1-54344] - This Anti-PIWIL2 antibody was used in Western Blot of Fetal Small Intestine tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

PIWIL2 antibody

PIWIL2 Rabbit Aff - Purified Hu P
50 µg / €480.00
  Novus Biologicals Inc.

PIWIL2 antibody

Immunohistochemistry-Paraffin: PIWIL2 Antibody [NBP1-85395] Immunohistochemical staining of human testis shows strong cytoplasmic positivity in seminiferous duct cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

16 Item(s)

per page
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