PIWIL4 antibody

Principal name

PIWIL4 antibody

Alternative names for PIWIL4 antibody

Piwi-like protein 4, HIWI2, PIWI, MIWI2

SwissProt ID

Q4G033 (Rat), Q7Z3Z4 (Human), Q8CGT6 (Mouse)

Gene ID

143689 (PIWIL4), 330890 (Piwil4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of PIWIL4 antibody

Kit Component:
- KN313350G1, Piwil4 gRNA vector 1 in pCas-Guide vector
- KN313350G2, Piwil4 gRNA vector 2 in pCas-Guide vector
- KN313350D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

"The induction of H3K9 methylation by PIWIL4 at the p16Ink4a locus." Sugimoto K., Kage H., Aki N., Sano A., Kitagawa H., Nagase T., Yatomi Y., Ohishi N., Takai D. Biochem. Biophys. Res. Commun. 359:497-502 (2007) [PubMed: 17544373]

18 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PIWIL4 antibody

Predicted band size: 97 kDa . Positive control: Hela, 231 and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Hela cell lysate Lane2: 231 cell lysate Lane3: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIWIL4 antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PIWIL4 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIWIL4 antibody

WB Suggested Anti-PIWIL4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human brain Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIWIL4 (N-term) antibody

WB Suggested Anti-PIWIL4 Antibody Titration: 0.2-1 ug/ml; Positive Control: HT1080 cell lysate Rabbit Aff - Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIWIL4 antibody

PIWIL4 Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

PIWIL4 antibody

Western Blot analysis of PIWIL4 expression in transfected 293T cell line (H00143689-T01) by PIWIL4 MaxPab polyclonal antibody.

Lane 1: PIWIL4 transfected lysate(93.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PIWIL4 antibody

Human Brain; WB Suggested Anti-PIWIL4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain; PIWIL4 antibody - N-terminal region (ARP52960_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PIWIL4 (N-term) antibody

Human HT1080; WB Suggested Anti-PIWIL4 Antibody Titration: 0.2-1 ug/ml. Positive Control: HT1080 cell lysate; PIWIL4 antibody - N-terminal region (ARP52961_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

Piwil4 - mouse gene knockout kit via CRISPR

Piwil4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Piwil4 - mouse gene knockout kit via CRISPR

Piwil4 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Piwil4 - mouse gene knockout kit via CRISPR

Piwil4 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PIWIL4

PIWIL4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PIWIL4 293T Cell Transient Overexpression Lysate(Denatured)

PIWIL4 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

PIWIL4 overexpression lysate

PIWIL4 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PIWIL4 (mGFP-tagged) - Human piwi-like 4 (Drosophila) (PIWIL4), 200 uL, >10^7 TU/mL

Lenti ORF particles, PIWIL4 (mGFP-tagged) - Human piwi-like 4 (Drosophila) (PIWIL4), 200 uL, >10^7 TU/mL  
200 µl / €1,810.00
  OriGene Technologies, Inc.

Lenti ORF particles, PIWIL4 (Myc-DDK tagged) - Human piwi-like 4 (Drosophila) (PIWIL4) , 200 uL, >10^7 TU/mL

Lenti ORF particles, PIWIL4 (Myc-DDK tagged) - Human piwi-like 4 (Drosophila) (PIWIL4) , 200 uL, >10^7 TU/mL  
200 µl / €1,630.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Piwil4 (GFP-tagged) - Mouse piwi-like homolog 4 (Drosophila) (Piwil4), 200 uL, >10^7 TU/mL

Lenti ORF particles, Piwil4 (GFP-tagged) - Mouse piwi-like homolog 4 (Drosophila) (Piwil4), 200 uL, >10^7 TU/mL  
200 µl / €1,610.00
  OriGene Technologies, Inc.

Lenti ORF particles, Piwil4 (Myc-DDK-tagged) - Mouse piwi-like homolog 4 (Drosophila) (Piwil4), 200 uL, >10^7 TU/mL

Lenti ORF particles, Piwil4 (Myc-DDK-tagged) - Mouse piwi-like homolog 4 (Drosophila) (Piwil4), 200 uL, >10^7 TU/mL  
200 µl / €1,450.00
  OriGene Technologies, Inc.

18 Item(s)

per page
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