PIWIL4 antibody

Principal name

PIWIL4 antibody

Alternative names for PIWIL4 antibody

Piwi-like protein 4, HIWI2, PIWI, MIWI2

SwissProt ID

Q4G033 (Rat), Q7Z3Z4 (Human), Q8CGT6 (Mouse)

Gene ID

143689 (PIWIL4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of PIWIL4 antibody

PIWIL4 belongs to the Argoute family of proteins; which function in development and maintence of germline stem cells. Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization; which is essential for the germline integrity. Acts via the piR metabolic process; which mediates the repression of transposable elements during meiosis by forming complexes composed of piRs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRs; a class of 24 to 30 nucleotide Rs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRs antisense and PIWIL2/MILI is required for such association.

General readings

"The induction of H3K9 methylation by PIWIL4 at the p16Ink4a locus." Sugimoto K., Kage H., Aki N., Sano A., Kitagawa H., Nagase T., Yatomi Y., Ohishi N., Takai D. Biochem. Biophys. Res. Commun. 359:497-502 (2007) [PubMed: 17544373]

14 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PIWIL4 antibody

Predicted band size: 97 kDa . Positive control: Hela, 231 and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Hela cell lysate Lane2: 231 cell lysate Lane3: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIWIL4 antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PIWIL4 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIWIL4 antibody

WB Suggested Anti-PIWIL4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human brain Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIWIL4 (N-term) antibody

WB Suggested Anti-PIWIL4 Antibody Titration: 0.2-1 ug/ml; Positive Control: HT1080 cell lysate Rabbit Aff - Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIWIL4 antibody

PIWIL4 Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

PIWIL4 antibody

Western Blot analysis of PIWIL4 expression in transfected 293T cell line (H00143689-T01) by PIWIL4 MaxPab polyclonal antibody.

Lane 1: PIWIL4 transfected lysate(93.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PIWIL4 antibody

Human Brain; WB Suggested Anti-PIWIL4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain; PIWIL4 antibody - N-terminal region (ARP52960_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PIWIL4 (N-term) antibody

Human HT1080; WB Suggested Anti-PIWIL4 Antibody Titration: 0.2-1 ug/ml. Positive Control: HT1080 cell lysate; PIWIL4 antibody - N-terminal region (ARP52961_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PIWIL4 antibody

Western Blot: PIWIL4 Antibody [NBP1-54342] - This Anti-PIWIL4 antibody was used in Western Blot of HT1080       tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

PIWIL4 antibody

Western Blot: PIWIL4 Antibody [NBP1-54343] - This Anti-PIWIL4 antibody was used in Western Blot of Fetal Brain tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

PIWIL4 antibody

Immunohistochemistry-Paraffin: PIWIL4 Antibody [NBP1-83491] Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in seminiferus duct cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PIWIL4

PIWIL4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PIWIL4 293T Cell Transient Overexpression Lysate(Denatured)

PIWIL4 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

PIWIL4 overexpression lysate

PIWIL4 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

14 Item(s)

per page
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