PKD2 antibody

Principal name

PKD2 antibody

Alternative names for PKD2 antibody

Polycystin-2, Polycystwin, R48321, Autosomal dominant polycystic kidney disease type II protein, Polycystic kidney disease 2 protein

SwissProt ID

O35245 (Mouse), Q09917 (Schpo), Q13563 (Human), Q4GZT3 (Bovin), Q9U1S7 (Caeel)

Gene ID

5311 (PKD2)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of PKD2 antibody

Transient receptor potential (TRP) channels are relatively non-selective ion channels ebling the exchange of cations down their electrochemical gradient. This exchange ebles the intracellular rise in + and Ca2+ concentration and ultimately in the cell membrane depolarization, important for action potential propagation and muscle contraction. They are activated by an extremely broad range of stimuli mely, temperature, voltage, pH, endocrine factors as well as sigling molecules. The TRP channel family is composed of 28 members divided in 7 subgroups: TRPV, TRPC, TRPM, TRPA, TRPN, TRPP and TRPML. All members of the TRP family have 6 transmembrane (TM) domains, and a pore domain between the fifth (S5) and sixth (S6) transmembrane domains. In general, TRP channels eble the passage of either + or Ca2+ ions with little or no preference. However, some channels do exhibit some selectivity. Also, TRP channels do not display the positive charges in the S4 voltage-sensing domain like most voltage sensitive channels, although they do display voltage dependency. In addition, TRP channels have in the C-termil intracellular region to the S6 domain a TRP domain comprising 25 amino acids that is more or less conserved among most TRP channels. Within the TRP domain, there is a TRP box composed of six amino acids, and TRP box 2 – a proline rich domain. The TRP domain seems to be responsible for the binding of PIP2, a phospholipid important for the regulation of channel activity. TRPP1 (polycystin-2, PC2, PKD2) belongs to the TRPP subfamily of TRP channels along with TRPP3 and TRPP5 proteins, and forms non-selective cation channels with different permeability to various divalent cations. The cellular localization of TRPP1 has been and still is the subject of a lasting debate. In many cell-types, TRPP1 is retained in the endoplasmic reticulum (ER) where it most likely functions as Ca2+ release channel, and with the help of cofactors, TRPP2 reaches the plasma membrane and the cilia. TRPP1 expression is widespread and is best characterized for its expression in the kidney where it is developmentally regulated. In the kidney, it associates with PKD1 (TRPP2) an eleven transmembrane-spanning protein (which does not belong to the TRP superfamily) to form functiol channels. In addition, TRPP1 is identified as one of the genes responsible for autosomal domint polycystic kidney disease (ADPKD).

General readings

Duning, K., et al. J. Biol. Chem. 285(44):33584-33588(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Stewart, A.P., et al. Biophys. J. 99(3):790-797(2010)
Petri, E.T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(20):9176-9181(2010)
Steiner, T.S., et al. Cell. Immunol. 264(2):135-142(2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PKD2 antibody

  Goat Anti-Polycystin 2 / PKD2 Antibody  
TA305931 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PKD2 antibody

  Anti-PKD2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human ovarian and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using PKD2 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKD2 antibody

  Rabbit Polyclonal Anti-TRPP1 (PKD2)  
Western blot analysis of rat kidney membrane proteins (lanes 1 and 3) and mouse kidney lysate (lanes 2 and 4): 1, 2. Anti-TRPP1 (PKD2) antibody, (1:200). 3, 4. Anti-TRPP1 (PKD2) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

PKD2 (C-term) antibody

Western blot analysis of PKD2 Antibody (C-term) Cat.-No AP53314PU-N in mouse lung tissue lysates (35ug/lane). This demonstrates the PKD2 antibody detected the PKD2 protein (arrow) Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PKD2 - human gene knockout kit via CRISPR

PKD2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PKD2 - human gene knockout kit via CRISPR

PKD2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PKD2 - human gene knockout kit via CRISPR

PKD2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PKD2 - human gene knockout kit via CRISPR

PKD2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PKD2 (mGFP-tagged)-Human polycystic kidney disease 2 (autosomal dominant) (PKD2), 200 uL, >10^7 TU/mL

Lenti ORF particles, PKD2 (mGFP-tagged)-Human polycystic kidney disease 2 (autosomal dominant) (PKD2), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PKD2 (Myc-DDK-tagged)-Human polycystic kidney disease 2 (autosomal dominant) (PKD2), 200 uL, >10^7 TU/mL

Lenti ORF particles, PKD2 (Myc-DDK-tagged)-Human polycystic kidney disease 2 (autosomal dominant) (PKD2), 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

10 Item(s)

per page
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