PKLR antibody

Principal name

PKLR antibody

Alternative names for PKLR antibody

PK1, PKL, Pyruvate kinase R/L, Pyruvate kinase 1, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase, Pyruvate kinase isozymes R/L

SwissProt ID

P12928 (Rat), P30613 (Human), P53657 (Mouse), Q29536 (Canfa)

Gene ID

5313 (PKLR)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Ce (C. elegans), Can (Canine), Rt (Rat), African clawed frog, Xen (Xenopus)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Flow Cytometry (F)

Background of PKLR antibody

Pyruvate kinase is also known as ATP:pyruvate phosphotransferase. At least three molecular forms with pyruvate kinase activity are known. PK1, the red cell form, is deficient in a type of hemolytic anemia. PK2 is found in kidney. PK3 is found in leukocytes, muscle, platelets, and brain but not in red cells or kidney. PK1 is found also in liver. During fetal development, PK3 changes to PK1 in the liver. PK1 is a tetramer composed of two dissimilar polypeptides of somewhat different molecular weight. It is an allosteric enzyme exhibiting cooperative binding for phosphoenolpyruvate and sensitivity to fructose-1,6-diphosphate. PK3 also is a tetrameric protein but, unlike PK1, all subunits are alike and, not unexpectedly, there is no cooperative behavior. The enzyme is insensitive to fructose-1,6-diphosphate. Patients with deficiency of red cell PK have normal PK2 and PK3. There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. Ab38237 recognizes the M1/M2 isoform of pyruvate kinase

General readings

Meza NW., et al. (2009). Mol Ther. 17(12):2000-9.
Roy MF., et al. (2007). J Exp Med. 204(12):2949-61.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PKLR antibody

Sample (30 ug of whole cell lysate) .   A: 293T.   B: A431.   C: HeLa.   D: HepG2.   E: A375.   7.5% SDS PAGE .   TA308453 diluted at 1:1000.     Rabbit IgG Aff - Purified Hu, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKLR antibody

Anti-PKLR mouse monoclonal antibody (TA501413) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR(RC206455). Mouse IgG2a OTI1H4 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PKLR antibody

Anti-PKLR mouse monoclonal antibody (TA501472) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR(RC206455). Mouse IgG2b OTI1C7 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PKLR antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (RC206455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR. Mouse IgG1 OTI1A5 Purified Hu WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PKLR antibody

Anti-PKLR mouse monoclonal antibody (TA501412) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR(RC206455). Mouse IgG2a OTI1F3 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PKLR antibody

Anti-PKLR mouse monoclonal antibody (TA501414) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR(RC206455). Mouse IgG2a OTI1H9 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PKLR antibody

Anti-PKLR mouse monoclonal antibody (TA501416) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR(RC206455). Mouse IgG2a OTI1H2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PKLR antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (RC206455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR. Mouse IgG2a OTI1B4 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PKLR antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (RC206455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR. Mouse IgG2b OTI1G9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PKLR antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT1E3 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

PKLR antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT1E3 Purified Hu E, WB
50 µl / €230.00
  Acris Antibodies GmbH

PKLR (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PKLR (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PKLR antibody

Immunoperoxidase of purified MaxPab antibody to PKLR on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

PKLR antibody

PKLR Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

PKLR antibody

PKLR MaxPab rabbit polyclonal antibody. Western Blot analysis of PKLR expression in mouse testis. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PKLR antibody

PKLR Mouse IgG2a 3B11 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PKLR antibody

PKLR Mouse IgG2b 4E12 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PKLR antibody

PKLR Mouse IgG2b 4A8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PKLR antibody

PKLR Mouse IgG2b 4C10 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

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