PKM2 antibody

Principal name

PKM2 antibody

Alternative names for PKM2 antibody

PK2, PK3, PKM, CTHBP, M2-PK, THBP1, OIP3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase muscle, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, Cytosolic thyroid hormone-binding protein, Opa-interacting protein 3

SwissProt ID

P11974 (Rabit), P11980 (Rat), P14618 (Human), P52480 (Mouse), Q91YI8 (Mouse), Q9BWB5 (Human)

Gene ID

100008676 (PKM), 5315, 5315350 (IPR004136)

Available reactivities

Rb (Rabbit), Hu (Human), Ms (Mouse), Mky (Monkey), Rt (Rat), Mam (Mammalia), Bov (Bovine), Eq (Equine), Por (Porcine), Ze (Zebrafish)

Available hosts

Goat, Mouse, Rabbit, Sheep

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R), Flow Cytometry (F)

Background of PKM2 antibody

Pyruvate kise, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2), an altertively-spliced variant of M1, is expressed during embryonic development. Studies found that cancer cells exclusively express PKM2. PKM2 is shown to be essential for aerobic glycolysis in tumors (Warburg effect). When the M2 isoform is switched to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased in cancer cells. These cells also show decreased tumorigenicity in mouse xenografts. Recent studies show that the oncogenic forms of FGFR1 directly phosphorylate Tyr105 of PKM2 and thereby inhibit the formation of active tetrameric PKM2. A PKM2 mutant found in cancer cells, in which Tyr105 is replaced by phenylalanine, leads to reduced cell proliferation in hypoxia and tumor growth in xenografts in nude mice. These findings suggest that the phosphorylation at Tyr105 is a critical switch for the metabolism in cancer cells that promotes tumor growth.

General readings

Ferguson E.C., et al. (2008) Trends Biochem Sci 33(8): 359-362Christofk H.R., et al. (2008) Nature 452(7184):181-186Shimada N., et al. (2008) Genes Cells 13(3): 245-254

  1. Quick-Filters:
  2. Cited (2)
  3. Highly Validated (9)

Items 1 to 20 of 77 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PKM2 (Isoform M1) antibody

Immunofluorescence staining of methanol-fixed MEF cells using PKM1 Antibody Cat.-No AP26075PU. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €270.00
  Acris Antibodies GmbH

PKM2 (Isoform M1) antibody

Immunofluorescence staining of methanol-fixed MEF cells using PKM1 Antibody Cat.-No AP26075PU. Rabbit Aff - Purified Hu, Ms WB
50 µl / €230.00
  Acris Antibodies GmbH

PKM2 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190259) Mouse IgG2b OTI1G10 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PKM2 mouse monoclonal antibody. (TA190260) Mouse IgG1 OTI1F12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PKM2 mouse monoclonal antibody. (TA190264) Mouse IgG1 OTI11B11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PKM2 Mouse monoclonal antibody. (TA190265) Mouse IgG1 OTI14C7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PKM2 mouse monoclonal antibody. (TA190266) Mouse IgG2b OTI12D5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PKM2 mouse monoclonal antibody. (TA190267) Mouse IgG1 OTI12B9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PKM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA190269) Mouse IgG1 OTI11G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

PKM2 Antibody : Western Blot analysis of HeLa lysates usinganti-PKM2. Image courtesy of Gregg Semenza - publication number 21620138 (PMID). Rabbit Purified Bov, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

Western blot analysis of extracts of various cell lines, using PKM2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PKM2 Isoform M1 specific antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKM1. Mouse IgG2b OTI3C6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 Isoform M1 specific antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PKM1 mouse monoclonal antibody. (TA190162) Mouse IgG1 OTI4E8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PKM2 mouse monoclonal antibody. (TA190262) Mouse IgG2a OTI1A1 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKM2 (RC201855, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKM2. Mouse IgG1 OTI14E1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKM2 (RC201855, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKM2. Mouse IgG1 OTI13H1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PKM2 mouse monoclonal antibody. (TA190271) Mouse IgG1 OTI1E7 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

Predicted band size: 58kDa. Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 antibody

Predicted band size: 58kDa. Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PKM2 (C-term) antibody

Western blot analysis of anti-h PKM2-N491 Pab (Cat. #TA324317) in Ramos cell line lysates (35ug/lane). hPKM2-N491(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms, Rt P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Items 1 to 20 of 77 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  • LinkedIn