PLA2G2A antibody

Principal name

PLA2G2A antibody

Alternative names for PLA2G2A antibody

PLA2B, PLA2L, RASF-A, NPS-PLA2, Group IIA phospholipase A2, Phospholipase A2 membrane associated, Phosphatidylcholine 2-acylhydrolase 2A, Non-pancreatic secretory phospholipase A2

SwissProt ID

P14422 (Rabit), P14423 (Rat), P14555 (Human), P31482 (Mouse), P47711 (Cavpo), Q56JZ2 (Bovin)

Gene ID

5320 (PLA2G2A), 18780 (Pla2g2a), 29692

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Western blot / Immunoblot (WB)

Background of PLA2G2A antibody

Phospholipase A2 (PLA2) catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. The same reaction also produces lysophosholipids, which represent another class of lipid mediators. The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of low-molecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense. This enzyme has been proposed to hydrolyze phosphatidylcholine (PC) in lipoproteins to liberate lyso-PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci. In mice, sPLA2 expression significantly influences HDL particle size and composition and demonstrate that an induction of sPLA2 is required for the decrease in plasma HDL cholesterol in response to inflammatory stimuli. Instillation of bacteria into the bronchi was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in rats.

18 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PLA2G2A antibody

Predicted band size: 16 kDa . Positive control: A549 and HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: A549 cell lysate Lane2: HeLa cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PLA2G2A antibody

TA348949 (0.1 ug/ml) staining of Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PLA2G2A antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLA2G2A (RC203250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLA2G2A.(1:2000) Mouse IgG1 OTI1C2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CRISPR

Catalog No. Species Donor Vector  

PLA2G2A - human gene knockout kit via CRISPR

PLA2G2A - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PLA2G2A - human gene knockout kit via CRISPR

PLA2G2A - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PLA2G2A - human gene knockout kit via CRISPR

PLA2G2A - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PLA2G2A - human gene knockout kit via CRISPR

PLA2G2A - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PLA2G2A

PLA2G2A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PLA2G2A (His-tagged Fusion Protein)

12% SDS-PAGE separation of Human sPLA2-IIA 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa 2. reduced and heated sample, 10µg/lane 3. non-reduced and non-heated sample, 10µg/lane Human Purified > 95 % >95% E. coli
0.1 mg / €470.00
  Acris Antibodies GmbH

PLA2G2A

PLA2G2A Human
2 µg / €300.00
  Abnova Taiwan Corp.

PLA2G2A

PLA2G2A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

PLA2G2A

PLA2G2A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

PLA2G2A

PLA2G2A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PLA2G2A

PLA2G2A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pla2g2a (GFP-tagged) - Mouse phospholipase A2, group IIA (platelets, synovial fluid) (Pla2g2a), 200 uL, >10^7 TU/mL

Lenti ORF particles, Pla2g2a (GFP-tagged) - Mouse phospholipase A2, group IIA (platelets, synovial fluid) (Pla2g2a), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Pla2g2a (Myc-DDK-tagged) - Mouse phospholipase A2, group IIA (platelets, synovial fluid) (Pla2g2a), 200 uL, >10^7 TU/mL

Lenti ORF particles, Pla2g2a (Myc-DDK-tagged) - Mouse phospholipase A2, group IIA (platelets, synovial fluid) (Pla2g2a), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pla2g2a (GFP-tagged ORF) - Rat phospholipase A2, group IIA (platelets, synovial fluid) (Pla2g2a), 200 uL, >10^7 TU/mL

Lenti ORF particles, Pla2g2a (GFP-tagged ORF) - Rat phospholipase A2, group IIA (platelets, synovial fluid) (Pla2g2a), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Pla2g2a (Myc-DDK-tagged ORF) - Rat phospholipase A2, group IIA (platelets, synovial fluid) (Pla2g2a), 200 uL, >10^7 TU/mL

Lenti ORF particles, Pla2g2a (Myc-DDK-tagged ORF) - Rat phospholipase A2, group IIA (platelets, synovial fluid) (Pla2g2a), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

18 Item(s)

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