Placenta growth factor / PGF antibody

Principal name

Placenta growth factor / PGF antibody

Alternative names for Placenta growth factor / PGF antibody

PGFL, PLGF, PlGF

SwissProt ID

P49763 (Human), P49764 (Mouse), Q63434 (Rat), Q9XS47 (Bovin)

Gene ID

5228 (PGF), 18654 (Pgf), 94203

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP)

Background of Placenta growth factor / PGF antibody

PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It belongs to the cysteine-knot superfamily. PLGF is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor. 
Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines. Alternate splicing results in at least three human mature PlGF forms containing 131 (PlGF1), 152 (PlGF2), and 203 (PlGF3) amino acids (aa) respectively. Only PlGF2 contains a highly basic heparinbinding 21 aa insert at the C-terminus. In the mouse, only one P lGF that is the equivalent of human PlGF2 has been identified. Human PlGF1 shares 56%, 55%, 74% and 95% aa identity with the appropriate isoform of mouse, rat, canine and equine PlGF. PlGF is mainly found as variably glycosylated, secreted, 55 - 60 kDa disulfide linked homodimers. Mammalian cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes and some endothelial cells. Circulating PlGF increases during pregnancy, reaching a peak in mid-gestation; this increase is attenuated in preeclampsia. However, deletion of PlGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia. PlGF binds and signals through VEGF R1/Flt1, but not VEGF R2/Flk-1/KDR, while VEGF binds both but signals only through the angiogenic receptor, VEGF R2. PlGF and VEGF therefore compete for binding to VEGF R1, allowing high PlGF to discourage VEGF/VEGF R1 binding and promote VEGF/VEGF R2mediated angiogenesis. However, PlGF (especially PlGF1) and some forms of VEGF can form dimers that decrease the angiogenic effect of VEGF on VEGF R2. PlGF2, but not PLGF-1, shows heparindependent binding of neuropilin (Npn)-1 and Npn2. PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing, but also contribute to inflammation in active sickle cell disease and atherosclerosis.

General readings

Cao Y, Ji WR, Qi P, Rosin A, Cao Y Placenta growth factor: identification and characterization of a novel isoform generated by RNA alternative splicing Biochem Biophys Res Commun. 1997 Jun 27;235(3):493-8.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Placenta growth factor / PGF antibody

  Rabbit anti-PGF Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using PGF antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Placenta growth factor / PGF antibody

Immunofluorescence with anti-Human PlGF antibodies (clones G10 and 3B10): Sf9 insect cells were infected with recombinant Baculovirus for Human PlGF-1 and PlGF-2. Three days after infection the Immunofluorescence protocol was performed. Mouse IgG1 342/3B10 Purified Hu C, ICC/IF, IP, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Placenta growth factor / PGF (PlGF-1/2) antibody

Immunofluorescence with anti-Human PlGF antibodies (clones G10 and 3B10): Sf9 insect cells were infected with recombinant Baculovirus for Human PlGF-1 and PlGF-2. Three days after infection the Immunofluorescence protocol was performed. Mouse IgG1 178/G10 Purified Hu E, IP, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

Placenta growth factor / PGF antibody

  Goat Anti-PLGF (placental growth factor) Antibody  
(1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Por E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Placenta growth factor / PGF antibody

  Rabbit polyclonal anti-PlGF-1 antibody  
Western blot analysis of PIGF using recombinant human PIGF. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Placenta growth factor / PGF antibody

  Rabbit polyclonal anti-PlGF antibody  
Western blot analysis using recombinant mouse PLGF. Lane 1: 50 ng, Lane 2: 250 ng, Lane 3: 1 ug. Rabbit Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Placenta growth factor / PGF antibody

  Biotinylated Anti-Human PlGF-1 Rabbit Polyclonal Antibody  
To detect hPlGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPlGF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Placenta growth factor / PGF antibody

  Anti-Human PlGF-1 Rabbit Polyclonal Antibody  
To detect hPlGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PlGF (TA328422) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPlGF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Placenta growth factor / PGF antibody

  Rabbit Polyclonal PLGF Antibody  
ELISA: Placental growth factor  Antibody - Affinity Purified Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

Placenta growth factor / PGF antibody

Placenta growth factor / PGF Mouse IgG1 PLGF94 Purified Hu
0.2 mg / €410.00
  Acris Antibodies GmbH

Placenta growth factor / PGF antibody

Placenta growth factor / PGF Mouse IgG1 PLGF94 Purified Hu
0.1 mg / €320.00
  Acris Antibodies GmbH

Placenta growth factor / PGF antibody

Placenta growth factor / PGF Mouse IgG1 PLGF94 Purified Hu
20 µg / €180.00
  Acris Antibodies GmbH

Placenta growth factor / PGF antibody

Placenta growth factor / PGF Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Placenta growth factor / PGF antibody

Placenta growth factor / PGF Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Placenta growth factor / PGF antibody

Placenta growth factor / PGF Rabbit Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Placenta growth factor / PGF antibody

Placenta growth factor / PGF Rabbit Aff - Purified Hu E, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

Placenta growth factor / PGF (N-term) antibody

Immunohistochemical analysis of paraffin embedded human placenta sections; staining in cytoplysm; DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Placenta growth factor / PGF antibody

Immunohistochemical staining of PlGF in paraffin-embedded mouse placenta embryo (a and b) showing intense cytoplasmic staining in mouse placenta at E9. In the embryo a positive signal was observed in endothelial structures of highly vascularized organs. Cross-reactivity of antibody was disproved as staining of human placenta did not reveal any signal©. Lower panel shows higher magnification of boxes in a-c.The experimentswereperformedbyDr. Frank Bicker fromtheresearchgroup „Molecular Signal Transduction“ (Prof. Dr. Mirko HH Schmidt), Institute ofMicroscopicAnatomyandNeurobiology, University Medical Center of Johannes Gutenberg University Mainz, Germany. Rabbit IgG Purified Ms ICC/IF, P, WB
0.2 mg / €370.00
  Acris Antibodies GmbH

Placenta growth factor / PGF antibody

Immunohistochemical staining of PlGF in paraffin-embedded mouse placenta embryo (a and b) showing intense cytoplasmic staining in mouse placenta at E9. In the embryo a positive signal was observed in endothelial structures of highly vascularized organs. Cross-reactivity of antibody was disproved as staining of human placenta did not reveal any signal©. Lower panel shows higher magnification of boxes in a-c.The experimentswereperformedbyDr. Frank Bicker fromtheresearchgroup „Molecular Signal Transduction“ (Prof. Dr. Mirko HH Schmidt), Institute ofMicroscopicAnatomyandNeurobiology, University Medical Center of Johannes Gutenberg University Mainz, Germany. Rabbit IgG Purified Ms ICC/IF, P, WB
0.1 mg / €270.00
  Acris Antibodies GmbH

Placenta growth factor / PGF antibody

Immunohistochemical staining of PlGF in paraffin-embedded mouse placenta embryo (a and b) showing intense cytoplasmic staining in mouse placenta at E9. In the embryo a positive signal was observed in endothelial structures of highly vascularized organs. Cross-reactivity of antibody was disproved as staining of human placenta did not reveal any signal ©. Lower panel shows higher magnification of boxes in a-c.The experiments were performed by Dr. Frank Bicker from the research group „Molecular Signal Transduction“ (Prof. Dr. Mirko HH Schmidt), Institute of Microscopic Anatomy and Neurobiology, University Medical Center of Johannes Gutenberg University Mainz, Germany. Rabbit IgG Aff - Purified Ms P, WB
50 µg / €280.00
  Acris Antibodies GmbH

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