Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)
Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of Plastin 3 / PLS3 antibody
Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
T-Plastin antibody detects T-Plastin protein at cytoplasm on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. T-Plastin antibody (GTX103323) diluted at 1:500.
T-Plastin antibody detects T-Plastin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with T-Plastin antibody (GTX103323) diluted at a dilution of 1:1000.
Human HT1080; WB Suggested Anti-PLS3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HT1080 cell lysate. PLS3 is supported by BioGPS gene expression data to be expressed in HT1080.; PLS3 antibody - middle region (ARP56623_P050) in Human HT1080 cells using Western Blot
Formalin-fixed and paraffin embedded rat cardiac muscle labeled with Rabbit Anti PLASTIN3/T Plastin Polyclonal Antibody, Unconjugated (bs-3643R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
> 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.