Platelet factor 4 / PF4 antibody

Principal name

Platelet factor 4 / PF4 antibody

Alternative names for Platelet factor 4 / PF4 antibody

PF-4, Iroplact, Oncostatin-A, CXCL4, SCYB4, C-X-C motif chemokine 4

SwissProt ID

P02776 (Human), P02777 (Bovin), P06765 (Rat), P30034 (Pig), P30035 (Sheep), P83470 (Rabit), Q9Z126 (Mouse)

Gene ID

5196 (PF4), 56744 (Pf4), 360918

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Rabbit, Mouse, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunodiffusion (ID), Immunoprecipitation (IP)

Background of Platelet factor 4 / PF4 antibody

Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity.

Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT) is a life-threatening complication that manifests itself in a small population of patients exposed to intravenous-heparin. It is characterized by the production of PF4/heparin auto-antibodies.
These auto-antibodies bind the PF4/heparin complexes and subsequently bind the FCGIIa receptor on the platelets surface through their Fc region. This activates the platelets and can initiate clot formation.

General readings

1. Arepally et al; PF4/heparin complexes are T cell-dependent antigens; Blood 106(3): 929-931, 2005.
2. Arepally GM, Kamei S, Park KS, et al. Characterization of a murine monoclonal antibody that mimics heparin-induced thrombocytopenia antibodies. Blood. 95(5):1533-1540, 2000.
3. Bharadwaj et al; A Novel Mutation in the Catalytic Domain That Reduces Tissue Factor Binding, Impairs Activation by Factor XA, and Abolishes Amidolytic and
Coagulant Activity; JBC 271(48) pp 30685-30691, 1996.
4. Yamamoto et al; Tissue Factor-dependent Autoactivation of Human Blood Coagulation Factor VII; JBC 267(27) 19089-19094, 1992.
5. Kazama et al; Isolation and Characterization of Proteolytic Fragments of Human Factor VIIa Which Inhibit the Tissue Factor-enhanced Amidolytic Activity of Factor
VIIa; The Journal of Biological Chemistry 286(22), pp 16231-16240, 1993.
6. Wojtukiewicz et al; Solid Tumor Cells Express Functional “Tethered Ligand” Thrombin Receptor; Cancer Research 55, 698-704, 1995.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Platelet factor 4 / PF4 antibody

  Biotinylated Anti-Human PF-4 Rabbit Polyclonal Antibody  
To detect hPF-4 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPF-4. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Platelet factor 4 / PF4 antibody

  Anti-Human PF-4 Rabbit Polyclonal Antibody  
To detect hPF-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PF-4 (TA328420) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPF-4. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Platelet factor 4 / PF4 antibody

Platelet factor 4 / PF4 Mouse IgG2b RTO Biotin Hu E, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Platelet factor 4 / PF4 antibody

Platelet factor 4 / PF4 Mouse IgG2b RTO Purified Hu E, WB
0.2 mg / €450.00
  Acris Antibodies GmbH

Platelet factor 4 / PF4 antibody

Platelet factor 4 / PF4 Mouse IgG2b KKO Biotin Hu E, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Platelet factor 4 / PF4 antibody

Platelet factor 4 / PF4 Mouse IgG2b KKO Purified Hu E, WB
0.2 mg / €450.00
  Acris Antibodies GmbH

Platelet factor 4 / PF4 antibody

Human Placenta (formalin-fixed, paraffin-embedded) stained with PF4 antibody AP22822PU-N at 5 µg/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen. Sheep IgG Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

Platelet factor 4 / PF4 antibody

Platelet factor 4 / PF4 Rabbit Serum Hu ID, IP
1 ml / €440.00
  Acris Antibodies GmbH

Platelet factor 4 / PF4 antibody

Platelet factor 4 / PF4 Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Platelet factor 4 / PF4 antibody

Platelet factor 4 / PF4 Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Platelet factor 4 / PF4 antibody

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. 
Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

Platelet factor 4 / PF4 antibody

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. 
Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PF4 - human gene knockout kit via CRISPR

PF4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PF4 - human gene knockout kit via CRISPR

PF4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PF4 - human gene knockout kit via CRISPR

PF4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PF4 - human gene knockout kit via CRISPR

PF4 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pf4 - mouse gene knockout kit via CRISPR

Pf4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pf4 - mouse gene knockout kit via CRISPR

Pf4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pf4 - mouse gene knockout kit via CRISPR

Pf4 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pf4 - mouse gene knockout kit via CRISPR

Pf4 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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