PLCG1 antibody

Principal name

PLCG1 antibody

Alternative names for PLCG1 antibody

PLC1, Phospholipase C-gamma-1, PLC-gamma-1, PLC gamma1 Phospholipase C-II, PLC-II, PLC-148, 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1, Phosphoinositide phospholipase C-gamma-1

SwissProt ID

P08487 (Bovin), P10686 (Rat), P19174 (Human), Q62077 (Mouse)

Gene ID

5335 (PLCG1)

Available reactivities

Hu (Human), Bov (Bovine), Rt (Rat), Xen (Xenopus), Ms (Mouse), Can (Canine), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F)

Background of PLCG1 antibody

Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε. PLC γ1 is widely distributed in bronchiolar epithelium, type I and II pneumocytes and fibroblasts of the interstitial tissue. Actinregulatory protein Villin is tyrosine phosphorylated and associates with PLC γ1 in the brush border of intestinal epithelial cells. Villin regulates PLC γ1 activity by modifying its own ability to bind phosphatidylinositol 4,5-biphosphate. PLC γ1 binds α1β1 Integrin and modulates α1β1 Integrin-specific adhesion. PLC γ1 and Ca2+ play a direct role in VEGF-regulated endothelial growth, however this signaling pathway is not linked to FGF-mediated effects in primary endothelial cells. PLC γ1 is rapidly activated in response to growth factor stimulation and plays an important role in regulating cell proliferation and differentiation, and may have a protective function during cellular response to oxidative stress.

General readings

DeBell KE, et al. Mol Cell Biol. 1999 Nov; 19(11): 7388-7398.
Verí MC, et al. Mol Cell Biol. 2001 Oct; 21(20): 6939-6950.
Sette C, et al. EMBO J. 2002 Oct 15; 21(20): 5386-5395.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PLCG (970-990) (incl. pos. control) antibody

Detection of endogenous Phospholipase C gamma: Whole cell lysates of EGF stimulated serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab AM20220PU-N PLCG antibody (Clone 2D5) at 0.5 µg/ml for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: A431 
Lane 2: A549 
Lane 3: SKOV3 
Lane 4: OVCAR5
Lane 5: HaCaT
Lane 6: PC3
Lane 7: HeLa
Lane 8: HepG2 Mouse IgG1 2D5 Purified Can, Hu, Ms, Rt WB
0.1 mg / €430.00
  Acris Antibodies GmbH

PLCG1+PLCG2 Control Peptide

PLCG1+PLCG2 Purified
50 µg / €190.00
  Acris Antibodies GmbH

PLCG1+PLCG2 antibody

Western blot analysis of PLCγ 1&2 expression in Jurkat cell lysate. Rabbit Aff - Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

PLCG1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PDGFRB and PLCG1. Mahlavu cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-PLCG1 mouse monoclonal antibody 1:100. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 2A2 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PLCG1 antibody

Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30min), using PLCG1 (Ab-771) antibody (#TA313086, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PLCG1 antibody

Predicted band size: 149 kDa . Positive control: Hela and 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2: Hela and 293 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PLCG1 antibody

Western blot analysis of PLCG1 expression in EGF treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PLCG1 antibody

Western blot analysis of PLCG1 expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PLCG1 pTyr783 antibody

Figure 1. Western blot analysis of extract from HL60 cells, untreated or treated with EGF using PLCG1 antibody (Phospho-Tyr783) Cat.-No AP02395PU-N/PU-S. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €320.00
  Acris Antibodies GmbH

PLCG1 pTyr783 antibody

Figure 1. Western blot analysis of extract from HL60 cells, untreated or treated with EGF using PLCG1 antibody (Phospho-Tyr783) Cat.-No AP02395PU-N/PU-S. Rabbit Aff - Purified Hu, Ms WB
50 µl / €240.00
  Acris Antibodies GmbH

PLCG1 antibody

Figure 1. Western blot analysis of extract from A431 cells, untreated or treated with EGF (200 ng/ml, 10 min), using PLCγ1 antibody AP02658PU (Lane 1 and 2) and PLCγ1 (phospho-Tyr783) antibody AP02395PU (Lane 3 and 4). Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €270.00
  Acris Antibodies GmbH

PLCG1 antibody

Figure 1. Western blot analysis of extract from A431 cells, untreated or treated with EGF (200 ng/ml, 10 min), using PLCγ1 antibody AP02658PU (Lane 1 and 2) and PLCγ1 (phospho-Tyr783) antibody AP02395PU (Lane 3 and 4). Rabbit Aff - Purified Hu, Ms WB
50 µl / €230.00
  Acris Antibodies GmbH

PLCG1 antibody

Immunohistochemistry (IHC) analyzes of PLCG1 antibody (Cat.-No AP06287PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PLCG1 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PLC-gamma 1 Antibody AP08095PU. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

PLCG1 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PLC-gamma 1 Antibody AP08095PU. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µl / €230.00
  Acris Antibodies GmbH

PLCG1 (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human Colon, lymphocytes PLCG1 antibody Cat.-No AP08580PU-N  at 1/25-1/100 followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Aff - Purified Hu, Ms, Rt IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

PLCG1 pTyr771 antibody

Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using PLCG1 pTyr771 Antibody (#AP09485PU, Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

PLCG1 pTyr771 antibody

Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using PLCG1 pTyr771 Antibody (#AP09485PU, Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µl / €240.00
  Acris Antibodies GmbH

PLCG1 antibody

Immunohistochemistry analyzes of PLC γ1 antibody (AP20282PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PLCG1 antibody

PLCG1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

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