Pleckstrin / PLEK antibody

Principal name

Pleckstrin / PLEK antibody

Alternative names for Pleckstrin / PLEK antibody

Platelet p47 protein

SwissProt ID

P08567 (Human), Q4KM33 (Rat), Q6Q308 (Canfa), Q9JHK5 (Mouse)

Gene ID

5341 (PLEK)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Immunoprecipitation (IP), Dot blot (Dot), Frozen Sections (C), Flow Cytometry (F)

Background of Pleckstrin / PLEK antibody

Platelet P47, also known as NSFL1C, UBX1, UBXD10 or UBXN2C, is a 370 amino acid protein that localizes to both the nucleus and the golgi apparatus (specifically to golgi stacks) and contains one SEP domain and one UBX domain. Functioning as part of a ternary complex with VCP (a protein involved in the heterotypic fusion of transport vesicles with their target membranes) and Syntaxin 5, p47 interacts with and reduces the ATPase activity of VCP and is required for the fragmentation of golgi stacks during mitosis and for subsequent reassembly of golgi stacks after mitosis. p47 is subject to phosphorylation during mitosis, which inhibits p47-golgi interaction and is, therefore, required for proper golgi stack formation and cisternal regrowth. Human p47 shares 89% sequence identity with its mouse counterpart, suggesting a conserved role between species. Multiple isoforms of p47 exist due to alternative splicing events.

General readings

Tyers M, Rachubinski RA, Stewart MI, Varrichio AM, Shorr RG, Haslam RJ, Harley CB.
Molecular cloning and expression of the major protein kinase C substrate of platelets.
Nature. 1988 Jun 2;333(6172):470-3.
PMID: 2897630

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Pleckstrin / PLEK antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PLEK mouse monoclonal antibody. (TA500891) Mouse IgG2b OTI4G6 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Pleckstrin / PLEK antibody

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-PLEKmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500899, Dilution 1:50) Mouse IgG2a OTI4F10 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Pleckstrin / PLEK antibody

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-PLEKmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500900, Dilution 1:50) Mouse IgG2b OTI2D11 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Pleckstrin / PLEK antibody

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PLEK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500902, Dilution 1:50) Mouse IgG1 OTI6E7 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Pleckstrin / PLEK antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PLEK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500903, Dilution 1:50) Mouse IgG2a OTI6H8 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Pleckstrin / PLEK antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PLEK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500916, Dilution 1:50) Mouse IgG1 OTI6B5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Pleckstrin / PLEK antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 6E3 Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Pleckstrin / PLEK (C-term) antibody

TA302907 staining (0.05µg/ml) of Human PBMC lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Pleckstrin / PLEK antibody

Sample(30 ug of whole cell lysate). A:Raji. 12% SDS PAGE. TA308338 diluted at 1:500 Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Pleckstrin / PLEK antibody

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PLEK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500901, Dilution 1:50) Mouse IgG2b OTI2F10 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Pleckstrin / PLEK antibody

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PLEK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500920, Dilution 1:50) Mouse IgG1 OTI3E2 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Pleckstrin / PLEK (337-350) antibody

Human Vessel, Platelets (formalin-fixed, paraffin-embedded) stained with PLEK antibody AP22534PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, L, Mky, Ms, Op, Prim, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Pleckstrin / PLEK antibody

PLEK polyclonal antibody (A01), Lot # 060814QCS1 Western Blot analysis of PLEK expression in THP-1 ( Cat # L007V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Pleckstrin / PLEK antibody

PLEK MaxPab polyclonal antibody. Western Blot analysis of PLEK expression in human placenta. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Pleckstrin / PLEK antibody

Pleckstrin / PLEK Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Pleckstrin / PLEK antibody

Western Blot analysis of PLEK expression in transfected 293T cell line (H00005341-T01) by PLEK MaxPab polyclonal antibody.

Lane 1: PLEK transfected lysate(40.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Pleckstrin / PLEK antibody

Immunoperoxidase of monoclonal antibody to PLEK on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1 ug/ml] Mouse IgG2a 6E3 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Pleckstrin / PLEK antibody

PLEK monoclonal antibody (M03), clone 2D8. Western Blot analysis of PLEK expression in HL-60 ( Cat # L014V1 ). Mouse IgG2a 2D8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Pleckstrin / PLEK antibody

PLEK polyclonal antibody ( Cat # PAB6527 ) staining ( 0.05 µg/ml ) of human PBMCs lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Pleckstrin / PLEK antibody

Human Spleen; WB Suggested Anti-PLEK Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Spleen; PLEK antibody - N-terminal region (ARP56397_P050) in Human Spleen cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

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