PLEKHM1 antibody

Principal name

PLEKHM1 antibody

Alternative names for PLEKHM1 antibody

KIAA0356, Pleckstrin homology domain-containing family M member 1, PH domain-containing family M member 1

SwissProt ID

Q5PQS0 (Rat), Q7TSI1 (Mouse), Q9Y4G2 (Human)

Gene ID

9842 (PLEKHM1), 353047 (Plekhm1)

Available reactivities

Hu (Human)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of PLEKHM1 antibody

PLEKHM1 is a member of the M family of Pleckstrin homolog domain-containing proteins, a group of proteins containing a RUN domain, two pleckstrin homology domains, and a cysteine-rich domain. It was identified through segregation analysis as a cause of osteopetrosis in humans. PLEKHM1 co-localizes with Rab7 to late endosomal/lysosomal vesicles in HEK293 and osteoclast-like cells, with this co-localization dependent on the prenylation of Rab7. Monocytes from a patient homozygous for a mutated form of PLEKHM1differentiated into osteoclasts normally, but failed to form ruffled borders and showed little evidence of bone resorbtion when cultured on dentine discs. Another mutation of PLEKHM1 impaired vesicular acidification and increased TRACP secretion in osteoclasts, suggesting that PLEKHM1 has critical roles in endosomal maturation and may be important in osteoclast-osteoblast cross-talk.

General readings

Edwards, T.L., et al. Ann. Hum. Genet. 74(2):97-109(2010)
Del Fattore, A., et al. J. Bone Miner. Res. 23(3):380-391(2008)
Van Wesenbeeck, L., et al. J. Clin. Invest. 117(4):919-930(2007)
Hartel-Schenk, S., et al. Glycoconj. J. 18 (11-12), 915-923 (2001)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PLEKHM1 antibody

  Rabbit Polyclonal PLEKHM1 Antibody  
Western blot analysis of PLEKHM1 in human lung tissue lysate with PLEKHM1 antibody at 1 µg/ml. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PLEKHM1 (N-term) antibody

Western blot analysis of PLEKHM1 Antibody (N-term) Cat.-No AP53352PU-N in K562 cell line lysates (35ug/lane). This demonstrates the PLEKHM1 antibody detected the PLEKHM1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PLEKHM1 - human gene knockout kit via CRISPR

PLEKHM1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PLEKHM1 - human gene knockout kit via CRISPR

PLEKHM1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PLEKHM1 - human gene knockout kit via CRISPR

PLEKHM1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PLEKHM1 - human gene knockout kit via CRISPR

PLEKHM1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Plekhm1 - mouse gene knockout kit via CRISPR

Plekhm1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Plekhm1 - mouse gene knockout kit via CRISPR

Plekhm1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Plekhm1 - mouse gene knockout kit via CRISPR

Plekhm1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Plekhm1 - mouse gene knockout kit via CRISPR

Plekhm1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PLEKHM1

  Recombinant protein of human pleckstrin homology domain containing, family M (with RUN domain) member 1 (PLEKHM1)  
PLEKHM1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PLEKHM1 overexpression lysate

PLEKHM1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PLEKHM1 (mGFP-tagged) - Human pleckstrin homology domain containing, family M (with RUN domain) member 1 (PLEKHM1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PLEKHM1 (mGFP-tagged) - Human pleckstrin homology domain containing, family M (with RUN domain) member 1 (PLEKHM1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.

Lenti ORF particles, PLEKHM1 (Myc-DDK tagged) - Human pleckstrin homology domain containing, family M (with RUN domain) member 1 (PLEKHM1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PLEKHM1 (Myc-DDK tagged) - Human pleckstrin homology domain containing, family M (with RUN domain) member 1 (PLEKHM1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €1,180.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Plekhm1 (GFP-tagged) - Mouse pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Plekhm1 (GFP-tagged) - Mouse pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), 200 uL, >10^7 TU/mL  
200 µl / €1,880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Plekhm1 (Myc-DDK-tagged) - Mouse pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Plekhm1 (Myc-DDK-tagged) - Mouse pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), 200 uL, >10^7 TU/mL  
200 µl / €1,690.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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