PLEKHM1 antibody

Principal name

PLEKHM1 antibody

Alternative names for PLEKHM1 antibody

KIAA0356, Pleckstrin homology domain-containing family M member 1, PH domain-containing family M member 1

SwissProt ID

Q5PQS0 (Rat), Q7TSI1 (Mouse), Q9Y4G2 (Human)

Gene ID

9842 (PLEKHM1), 353047 (Plekhm1)

Available reactivities

Hu (Human)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of PLEKHM1 antibody

PLEKHM1 is a member of the M family of Pleckstrin homolog domain-containing proteins, a group of proteins containing a RUN domain, two pleckstrin homology domains, and a cysteine-rich domain. It was identified through segregation analysis as a cause of osteopetrosis in humans. PLEKHM1 co-localizes with Rab7 to late endosomal/lysosomal vesicles in HEK293 and osteoclast-like cells, with this co-localization dependent on the prenylation of Rab7. Monocytes from a patient homozygous for a mutated form of PLEKHM1differentiated into osteoclasts normally, but failed to form ruffled borders and showed little evidence of bone resorbtion when cultured on dentine discs. Another mutation of PLEKHM1 impaired vesicular acidification and increased TRACP secretion in osteoclasts, suggesting that PLEKHM1 has critical roles in endosomal maturation and may be important in osteoclast-osteoblast cross-talk.

General readings

Edwards, T.L., et al. Ann. Hum. Genet. 74(2):97-109(2010)
Del Fattore, A., et al. J. Bone Miner. Res. 23(3):380-391(2008)
Van Wesenbeeck, L., et al. J. Clin. Invest. 117(4):919-930(2007)
Hartel-Schenk, S., et al. Glycoconj. J. 18 (11-12), 915-923 (2001)

13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PLEKHM1 antibody

Western blot analysis of PLEKHM1 in human lung tissue lysate with PLEKHM1 antibody at 1 µg/ml. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PLEKHM1 Control Peptide

PLEKHM1
50 µg / €160.00
  Acris Antibodies GmbH

PLEKHM1 (N-term) antibody

Western blot analysis of PLEKHM1 Antibody (N-term) Cat.-No AP53352PU-N in K562 cell line lysates (35ug/lane). This demonstrates the PLEKHM1 antibody detected the PLEKHM1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PLEKHM1 antibody

Detection limit for recombinant GST tagged PLEKHM1 is approximately 1ng/ml as a capture antibody. Mouse IgG2b 1C9 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

CRISPR

Catalog No. Species Donor Vector  

PLEKHM1 - human gene knockout kit via CRISPR

PLEKHM1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PLEKHM1 - human gene knockout kit via CRISPR

PLEKHM1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PLEKHM1 - human gene knockout kit via CRISPR

PLEKHM1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PLEKHM1 - human gene knockout kit via CRISPR

PLEKHM1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Plekhm1 - mouse gene knockout kit via CRISPR

Plekhm1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PLEKHM1

PLEKHM1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PLEKHM1

PLEKHM1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PLEKHM1

PLEKHM1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PLEKHM1 overexpression lysate

PLEKHM1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

13 Item(s)

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