PLK1 antibody

Principal name

PLK1 antibody

Alternative names for PLK1 antibody

Polo-like kinase 1, PLK-1, STPK13, Serine/threonine-protein kinase PLK1

SwissProt ID

P34331 (Caeel), P53350 (Human), P70032 (Xenla), Q07832 (Mouse), Q2TA25 (Bovin), Q62673 (Rat)

Gene ID

5347 (PLK1), 4534780 (P94968), 380481 (plk1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Xen (Xenopus), Ze (Zebrafish), Por (Porcine), Chimp (Chimpanzee), Chk (Chicken), Ce (C. elegans), Prim (Primate)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ELISA (detection) (E(detection)), PLA/Proximity ligation assay (PLA), ChIP assay (ChIP), Flow Cytometry (F), Luminex (LMNX)

Background of PLK1 antibody

At least four distinct polo-like kises exist in mammalian cells: PLK1, PLK2, PLK3, and PLK4/SAK . PLK1 apparently plays many roles during mitosis, particularly in regulating mitotic entry and exit. The mitosis promoting factor (MPF), cdc2/cyclin B1, is activated by dephosphorylation of cdc2 (Thr14/Tyr15) by cdc25C. PLK1 phosphorylates cdc25C at Ser198 and cyclin B1 at Ser133 causing translocation of these proteins from the cytoplasm to the nucleus. PLK1 phosphorylation of Myt1 at Ser426 and Thr495 has been proposed to ictivate Myt1, one of the kises known to phosphorylate cdc2 at Thr14/Tyr15. Polo-like kises also phosphorylate the cohesin subunit SCC1, causing cohesin displacement from chromosome arms that allow for proper cohesin localization to centromeres. Mitotic exit requires activation of the aphase promoting complex (APC), a ubiquitin ligase responsible for removal of cohesin at centromeres, and degradation of securin, cyclin A, cyclin B1, Aurora A, and cdc20. PLK1 phosphorylation of the APC subunits Apc1, cdc16, and cdc27 has been demonstrated in vitro and has been proposed as a mechanism by which mitotic exit is regulated.Substitution of Thr210 with Asp has been reported to elevate PLK1 kise activity and delay/arrest cells in mitosis, while a Ser137Asp substitution leads to S-phase arrest. In addition, while D damage has been found to inhibit PLK1 kise activity, the Thr210Asp mutant is resistant to this inhibition. PLK1 has been reported to be phosphorylated in vivo at Ser137 and Thr210 in mitosis; D damage prevents phosphorylation at these sites.

General readings

1. Erikson E, Haystead TAJ, Qian, Y-W, Maller JL (2004) A feedback loop in the polo-like kinase activation pathway. J
Biol Chem 279:32219-32224.
2. Kumagai A, Dunphy WG (1996) Purification and molecular cloning of Plx1, a cdc25-regulatory kinase from Xenopus
egg extracts. Science 273:1377-1380.
3. Liu J, Lewellyn AL, Chen LG, Maller JL (2004) The polo box is required for multiple functions of Plx1 in mitosis. J Biol
Chem 279:21367-21373.
4. van Vugt, MATM, van de Weerdt, BCM, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RMF, Medema RH (2004) Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/cdc20 activation and initiation of cytokinesis. J Biol Chem 279:36841-36854.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PLK1 pThr210 antibody

Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with the pan-PLK mAb (clone 3F8), resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary Ab and a chemiluminescence detection system. Mouse IgG1 2A3 Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PLK1 pThr210 antibody

Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with the pan-PLK mAb (clone 3F8), resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary Ab and a chemiluminescence detection system. Mouse IgG1 2A3 Aff - Purified Hu P, WB
25 µg / €230.00
  Acris Antibodies GmbH

PLK1 pThr210 antibody

Figure 1. Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 k PLK protein phosphorylated at Thr210 (Control). The phosphospecificity of this    labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Bov, Can, Hu, Ms, non-Human Primates, Rt, Xen, Ze WB
0.1 ml / €380.00
  Acris Antibodies GmbH

PLK1 pThr210 antibody

WB analysis using Anti-Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1:500 dilution of the antibody at RTfollowed by detection using standard techniques. Rabbit Aff - Purified Can, Chimp, Chk, Hu, Ms, Por, Rt, Xen, Ze E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PLK1 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500383) Mouse IgG1 OTI3F12 Purified Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PLK1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500389) Mouse IgG2b OTI1D4 Purified Hu IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PLK1 antibody

Anti-PLK1 mouse monoclonal antibody (TA500393) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PLK1(RC201795). Mouse IgG1 OTI8C12 Purified Can, Hu, Mky F, ICC/IF, IP, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PLK1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395) Mouse IgG2b OTI1A2 Purified Hu IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PLK1 antibody

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500396) Mouse IgG2b OTI5C1 Purified Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PLK1 antibody

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500401) Mouse IgG2b OTI6C6 Purified Can, Hu IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PLK1 antibody

PLK1 ELISA with 8C12 Capture (TA600111) and 1A2 Detection (TA700111) Antibodies. Substrate used: Recombinant Human PLK1 (TP301795) Mouse IgG1 OTI8C12 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

PLK1 (161-440) antibody

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Hu, Rt P, WB
50 µl / €470.00
  Acris Antibodies GmbH

PLK1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CCNB1 and PLK1. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-PLK1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 2G12 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PLK1 antibody

Immunofluorescence of monoclonal antibody to PLK1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 4G11 Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PLK1 antibody

Immunohistochemistry of PLK1 (phospho T210) polyclonal antibody ( Cat # PAB10069 ) was used at a 1 : 200 dilution to detect PLK1 by immunohistochemistry in human colon carcinoma tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Alan Yen,Life Span Biosciences, Seattle, WA. Rabbit Chimp, Chk, Hu, Ms, Rt E, P, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

PLK1 antibody

Immunocytochemistry: PLK1 Antibody [NB100-547] -  NBF-fixed asynchronous HeLa cells. Affinity purified rabbit anti-PLK1 used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100. Rabbit Aff - Purified Hu ICC/IF, IP, PLA, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

PLK1 antibody

Immunohistochemistry-Paraffin: PLK1 Antibody (1D4) [NBP1-48291] - Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-PLK1mouse monoclonal antibody. (Dilution 1:50) Mouse 1D4 Aff - Purified Hu IP, P, WB
0.1 ml / €530.00
  Novus Biologicals Inc.

PLK1 antibody

Immunohistochemistry-Paraffin: PLK1 [p Thr210] Antibody [NBP1-78021] Affinity Purified Plk1 pT210 was used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded. Rabbit Aff - Purified Hu C, E, P, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

PLK1 (287-298) antibody

Staining of HeLa nuclear lysate using PLK1 ANtibody Cat.-No AP32818PU-N at 0.3 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

PLK1 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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