PLK1 antibody

Principal name

PLK1 antibody

Alternative names for PLK1 antibody

Polo-like kinase 1, PLK-1, STPK13, Serine/threonine-protein kinase PLK1

SwissProt ID

P34331 (Caeel), P53350 (Human), P70032 (Xenla), Q07832 (Mouse), Q2TA25 (Bovin), Q62673 (Rat)

Gene ID

5347 (PLK1), 18817 (Plk1), 25515

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Xen (Xenopus), Ze (Zebrafish), Chimp (Chimpanzee), Chk (Chicken), Por (Porcine), Frog

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Luminex (LMNX)

Background of PLK1 antibody

At least four distinct polo-like kises exist in mammalian cells: PLK1, PLK2, PLK3, and PLK4/SAK . PLK1 apparently plays many roles during mitosis, particularly in regulating mitotic entry and exit. The mitosis promoting factor (MPF), cdc2/cyclin B1, is activated by dephosphorylation of cdc2 (Thr14/Tyr15) by cdc25C. PLK1 phosphorylates cdc25C at Ser198 and cyclin B1 at Ser133 causing translocation of these proteins from the cytoplasm to the nucleus. PLK1 phosphorylation of Myt1 at Ser426 and Thr495 has been proposed to ictivate Myt1, one of the kises known to phosphorylate cdc2 at Thr14/Tyr15. Polo-like kises also phosphorylate the cohesin subunit SCC1, causing cohesin displacement from chromosome arms that allow for proper cohesin localization to centromeres. Mitotic exit requires activation of the aphase promoting complex (APC), a ubiquitin ligase responsible for removal of cohesin at centromeres, and degradation of securin, cyclin A, cyclin B1, Aurora A, and cdc20. PLK1 phosphorylation of the APC subunits Apc1, cdc16, and cdc27 has been demonstrated in vitro and has been proposed as a mechanism by which mitotic exit is regulated.Substitution of Thr210 with Asp has been reported to elevate PLK1 kise activity and delay/arrest cells in mitosis, while a Ser137Asp substitution leads to S-phase arrest. In addition, while D damage has been found to inhibit PLK1 kise activity, the Thr210Asp mutant is resistant to this inhibition. PLK1 has been reported to be phosphorylated in vivo at Ser137 and Thr210 in mitosis; D damage prevents phosphorylation at these sites.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PLK1 pThr210 antibody

  Mouse Monoclonal anti-PLK-1 Phospho (Thr210) Antibody  
Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with the pan-PLK mAb (clone 3F8), resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary Ab and a chemiluminescence detection system. Mouse IgG1 2A3 Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PLK1 pThr210 antibody

  Rabbit polyclonal Plk1 phospho T210 antibody  
WB analysis using Anti-Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1:500 dilution of the antibody at RTfollowed by detection using standard techniques. Rabbit Aff - Purified Can, Chimp, Chk, Hu, Ms, Por, Rt, Xen, Ze E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

PLK1 antibody

  anti-PLK1 mouse monoclonal antibody, clone OTI3F12 (formerly 3F12)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500383) Mouse IgG1 OTI3F12 Purified Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PLK1 antibody

  PLK1 mouse monoclonal antibody, clone OTI1D4 (formerly 1D4)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500389) Mouse IgG2b OTI1D4 Purified Hu IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PLK1 antibody

  anti-PLK1 mouse monoclonal antibody, clone OTI8C12 (formerly 8C12)  
Anti-PLK1 mouse monoclonal antibody (TA500393) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PLK1(RC201795). Mouse IgG1 OTI8C12 Purified Can, Hu, Mky F, ICC/IF, IP, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PLK1 antibody

  PLK1 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395) Mouse IgG2b OTI1A2 Purified Hu IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PLK1 antibody

  anti-PLK1 mouse monoclonal antibody, clone OTI5C1 (formerly 5C1)  
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500396) Mouse IgG2b OTI5C1 Purified Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PLK1 antibody

  PLK1 mouse monoclonal antibody, clone OTI6C6 (formerly 6C6)  
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500401) Mouse IgG2b OTI6C6 Purified Can, Hu IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PLK1 antibody

  Elisa and Luminex Validated and PLK1 mouse monoclonal antibody (clone 8C1, clone OTI8C12 (formerly 8C12)  
PLK1 ELISA with 8C12 Capture (TA600111) and 1A2 Detection (TA700111) Antibodies. Substrate used: Recombinant Human PLK1 (TP301795) Mouse IgG1 OTI8C12 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

PLK1 (161-440) antibody

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

PLK1 (287-298) antibody

Staining of HeLa nuclear lysate using PLK1 ANtibody Cat.-No AP32818PU-N at 0.3 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PLK1 (C-term) antibody

  PLK1 (C-term) Receptor Rabbit Polyclonal Antibody  
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PLK1 antibody

  Rabbit Anti-Polo-Like Kinase (Thr210) Antibody (Phospho-Specific)  
Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 k PLK protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK antibody. The immunolabeling is completely eliminated by treatment with λ-Ptase. Rabbit IgG Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PLK1 antibody

  Rabbit Anti-Polo-Like Kinase Kinase (Ser482,486,490) Antibody (Phospho-Specific)  
PLK1 Rabbit IgG Purified Xen WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PLK1 antibody

  PLK1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of HeLa cell lines, using PLK1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PLK1 antibody

  Rabbit Polyclonal anti-PLK-1 Phospho (Thr210)  
Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with the pan-PLK monoclonal antibody, then western blotted with Poly6186 against phosphorylated PLK-1 (Thr210).  Panel B. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) in Panel A were stripped and re-probed with a polyclonal antibody against pan-PLK-1 (Poly6185) to document equivalent loading of the PLK-1 protein. Rabbit IgG Poly6186 Purified Hu ICC/IF, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

PLK1 antibody

  Mouse Monoclonal anti-PLK-1 Antibody  
Hela cell extract  (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Plk-1 (Clone 3F8) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 3F8 Purified Hu, Ms ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PLK1 antibody

  Rabbit Polyclonal PLK1 Antibody  
Western Blot: PLK1 Antibody [TA336437] - analysis of HCT-116 lysate using PLK1 antibody at 1:2000. Rabbit Purified Bov, Can, Hu, Ms, Por, Rt P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

PLK1 antibody

  Rabbit Polyclonal Anti-PLK1 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (PLK1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PLK1 antibody

  Mouse monoclonal Anti-Plx Clone AZ24  
PLK1 Mouse IgG2 AZ24 Purified Frog, Hu, Ms E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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