PMEPA1 / STAG1 antibody

Principal name

PMEPA1 / STAG1 antibody

Alternative names for PMEPA1 / STAG1 antibody

TMEPAI, Transmembrane prostate androgen-induced protein, Solid tumor-associated 1 protein

SwissProt ID

Q969W9 (Human), Q9D7R2 (Mouse)

Gene ID

56937 (PMEPA1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of PMEPA1 / STAG1 antibody

The abnormal activation of the epidermal growth factor (EGF) pathway is one of the most common findings in human cancer, and a number of molecular devices of laboratory and clinical relevance have been designed to block this transduction pathway. The activation of 4 EGF receptor family members resulted in to large number of cellular events that might be regulating the metastasis and cell growth. The identification of new molecular targets working downstream of these pathways may provide new sites for therapeutic interventions for cancer diagnosis and potentially prevention and therapy. Several EGF target genes have been identified, one of them is Erg1.2 a mouse homolog of the solid tumor associated gene STAG1. Both in humans and in mice, it belongs to a new gene family that can give origin to several protein isoforms through alternative splicing and/or multiple translation starts. Sequence analysis and experimental data suggest that ERG1.2 is likely to function as a membrane-bound protein interacting with downstream signaling molecules through WW- and SH3-binding domains. Other members of this family include TMEPA1, and TPD52. PMEPA1 was identified originally as a highly androgen-inducible gene with prostate-abundant expression that was restricted to prostatic epithelial cells. PMEPA1 protein is a NEDD4 (ubiquitin-protein isopeptide ligase)-binding protein, which negatively regulates prostate cancer cell growth (1). During prostate cancer progression TMEPA1 gene transcription is reduced or lost suggesting a direct role of epigenetic events in this process.
PMEPA1 negatively regulates AR protein levels in different cell culture models. Transient expression of PMEPA1 down-regulates AR protein levels and AR transcriptional targets in prostate cancer cells. Conversely, knockdown of PMEPA1 leads to elevated levels of AR protein, AR transcriptional targets (prostate-specific antigen), and increased cell cycle S phase. The TMEPA1 mutant cells are impaired in NEDD4 recruitment shows attenuated AR ubiquitination and AR protein down-regulation.
Certain epigenetic cascade events contributes to the selective growth advantage during tumor progression. During prostate cancer progression the TMEPA1 gene is reduced or lost as a result of DNA methylation of SP1 sites within the PMEPA1 promoter may also contribute to the repression of PMEPA1 gene (2). The TMEPA1 negatively regulates the stability of AR protein by enhancing AR ubiquitination and proteosome-mediated degradation through NEDD4 and the TMEPA1-AR degradation pathway may represent a new androgen-dependent mechanism for regulating AR levels in prostate epithelial cells. The decrease in TMEPA1 in prostate cancers may lead to an increase in AR function and strengthen the biological role of TMEPA1 in prostate cancers. TMEPA1 is a 254 amino acid (apparent MW 30-31kDa) protein highly expressed in prostate cells.

19 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PMEPA1 / STAG1 antibody

Western blot analysis of extracts from HT-29 cells, using TMEPA antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PMEPA1 / STAG1 antibody

Western blot analysis of PMEPA1 inA549 cell lysate with PMEPA1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PMEPA1 / STAG1 Control Peptide

PMEPA1 / STAG1
0.1 mg / €250.00
  Acris Antibodies GmbH

PMEPA1 / STAG1 antibody

PMEPA1 / STAG1 Rabbit IgG Aff - Purified Hu Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PMEPA1 / STAG1 antibody

Human Prostate, Smooth Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PMEPA1 / STAG1 antibody

Detection limit for recombinant GST tagged TMEPAI is approximately 1ng/ml as a capture antibody. Mouse IgG2a 2A12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PMEPA1 / STAG1 antibody

Immunohistochemistry analysis of paraffin-embedded human prostate tissue, using TMEPA Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

PMEPA1 / STAG1 antibody

Immunohistochemistry analysis of paraffin-embedded human prostate tissue, using TMEPA Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

PMEPA1 / STAG1 antibody

Immunohistochemical analysis of PMEPA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

PMEPA1 / STAG1 antibody

Immunohistochemical analysis of PMEPA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

PMEPA1 / STAG1 antibody

Immunohistochemical analysis of PMEPA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

PMEPA1 / STAG1 antibody

PMEPA1 / STAG1 Rabbit IgG Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

PMEPA1 / STAG1 antibody

PMEPA1 / STAG1 Rabbit IgG Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PMEPA1 / STAG1 (transcript variant 4)

PMEPA1 / STAG1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PMEPA1 / STAG1 (transcript variant 3)

PMEPA1 / STAG1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PMEPA1 / STAG1

PMEPA1 / STAG1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PMEPA1 / STAG1

PMEPA1 / STAG1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PMEPA1 overexpression lysate

PMEPA1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

PMEPA1 overexpression lysate

PMEPA1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

19 Item(s)

per page
  • LinkedIn