PML / RNF71 antibody

Principal name

PML / RNF71 antibody

Alternative names for PML / RNF71 antibody

RING finger protein 71, MYL, TRIM19, Probable transcription factor PML, Tripartite motif-containing protein 19

SwissProt ID

P29590 (Human), Q60953 (Mouse)

Gene ID

5371 (PML), 18854 (Pml)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Frozen Sections (C), Flow Cytometry (F)

Background of PML / RNF71 antibody

PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. PML localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic sigls. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive altertive splicing of this gene results in several variations of the protein's central and C-termil regions; all variants encode the same N-terminus. The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic sigls. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive altertive splicing of this gene results in several variations of the protein's central and C-termil regions; all variants encode the same N-terminus. Altertively spliced transcript variants encoding different isoforms have been identified.

General readings

1. Sone, M., et al., J. Cell Sci. 120, 2498-2506 (2007).
2. Keyes, W. M., et al., Genes & Dev. 19, 1986-1999 (2005).
3. Gaumont-Leclerc, M-F., et al., J. Biol. Chem. 279, 46802-46809 (2004).
4. Stanchina, E., et al., Mol. Cell 13, 523-535 (2004).
Clone 36-1-104 is used in these references.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PML / RNF71 antibody

Immunocytochemical detection of Mouse PML in P19 with AM20293AF-N PML antibody. Mouse IgG2b 36-1-104 Azide Free Ms ICC/IF, IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

PML / RNF71 antibody

Western blot analysis of extracts from A549 cells, using PML antibody (#TA314354).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PML / RNF71 antibody

Western blot analysis of HeLa cell lysate using PML antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

PML / RNF71 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and PML HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PML mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 1D12 Purified Hu, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PML / RNF71 (37-51) antibody

AP15736PU PML antibody staining of Human Lymph Node. Rabbit Purified Hu P
0.5 ml / €300.00
  Acris Antibodies GmbH

PML / RNF71 (37-51) antibody

AP15736PU PML antibody staining of Human Lymph Node. Rabbit Purified Hu P
1 ml / €410.00
  Acris Antibodies GmbH

PML / RNF71 (37-51) antibody

AP15736PU PML antibody staining of Human Lymph Node. Rabbit Purified Hu P
0.1 ml / €170.00
  Acris Antibodies GmbH

PML / RNF71 antibody

WB Suggested Anti-PML Antibody Titration: 1 ug/ml; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PML / RNF71 antibody

WB Suggested Anti-PML Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PML / RNF71 antibody

PML antibody - middle region validated by WB using Neuroblastoma at 1: 500. Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PML / RNF71 antibody

WB using the antibody against PML. The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML protein. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

PML / RNF71 antibody

Figure.1 Western blot analysis of extracts from K562 cells using anti-PML antibody. Mouse IgG1 B3E9 Aff - Purified Hu WB
0.5 mg / €470.00
  Acris Antibodies GmbH

PML / RNF71 antibody

PML / RNF71 Mouse IgG1 B4C8 Aff - Purified Hu WB
0.5 mg / €470.00
  Acris Antibodies GmbH

PML / RNF71 antibody

Immunocytochemical detection of PML in acetone fixed HEp-II with AM26594AF-N. Mouse IgG1 1B9 Azide Free Hu F, ICC/IF, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

PML / RNF71 antibody

Immunohistochemistry (IHC) analyzes of PML antibody (Cat.-No.: AP06288PU-N) in paraffin-embedded human tonsil thyroid tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PML / RNF71 (Sumoylation Site) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PML / RNF71 (37-51) antibody

Human Breast (formalin-fixed, paraffin-embedded) stained with PML antibody AP22715PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu P
50 µg / €470.00
  Acris Antibodies GmbH

PML / RNF71 (B-Box 1 Dom.) antibody

Western blot analysis of extracts from NB4 cells using anti-PML antibody. Rabbit Aff - Purified Hu WB
0.1 mg / €300.00
  Acris Antibodies GmbH

PML / RNF71 (Ring Finger Dom.) antibody

Western blot analysis of extracts from NB4 cells using anti-PML polyclonal antibody. Rabbit Aff - Purified Hu ICC/IF, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

PML / RNF71 antibody

PML / RNF71 Rabbit Serum Ms C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

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