PMS2 antibody

Principal name

PMS2 antibody

Alternative names for PMS2 antibody

PMSL2, Mismatch repair endonuclease PMS2, DNA mismatch repair protein PMS2, PMS1 protein homolog 2

SwissProt ID

P54278 (Human), P54279 (Mouse)

Gene ID

5395 (PMS2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Hst (Hamster), Bov (Bovine), Chk (Chicken)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PMS2 antibody

Mismatch DNA repair in bacteria is carried out by the MutL, MutH, and MutS proteins. The MutS protein initially bunds to mismatched DNA. This is followed by binding of the MutH endonuclease and MutL to form a complex that carries out excision repair. The hMSH2 gene specifies a MutS homologue and hMLH1, hPMS1, and hPMS2 encode MutL homologs. Mutations in these genes are associated with hereditary nonpolyposis colon cancer (HNPCC), one of the most common hereditary diseases in man. As with the bacterial system, HNPCC is characterized by frequent microsatellite mutations that arise by somatic mutation due to a replication error (RER+) phenotype. Both hPMS1 and hPMS2 are mutated in the germline of HNPCC patients. Although the exact function of MutL and its homologs has yet to be determined, it is known that a complex of PMS2 and MLH1 (MutLa) from HeLa cells can complement a deficiency of MLH1 in hypermutable H6 colorectal tumor cells.

General readings

1. Nicolaides N.C., et al. (1994) Mutations of two PMS homologues in hereditary nonpolyposis colon cancer. Nature 371:75-80.
2. Wang Y., Cortez D., Yazdi P., Neff N., Elledge S.J., Qin J.; (2000) BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 14:927-939.
3. Yuan Z.Q., et al. (2002) Polymorphisms and HNPCC: PMS2-MLH1 protein interactions diminished by single nucleotide polymorphisms. Hum. Mutat. 19:108-113.
4. Nakagawa,H., et al. (2004) Mismatch repair gene PMS2: disease-causing germline mutations are frequent in patients whose tumors stain negative for PMS2 protein, but paralogous genes obscure mutation detection and interpretation. Cancer Res. 64 (14), 4721-4727.
5. Luo,Y., Lin,F.T. and Lin,W.C. (2004) ATM-mediated stabilization of hMutL DNA mismatch repair proteins augments p53 activation during DNA damage. Mol. Cell. Biol. 24 (14), 6430-6444.
6. Lucci-Cordisco, E. et al. (2003) Hereditary nonpolyposis colorectal cancer and related conditions. Am. J. Med. Genet. 122A (4), 325-334.
De Jong, A.E. et al. (2004) Microsatellite Instability, Immunohistochemistry, and Additional PMS2 Staining in Suspected Hereditary Nonpolyposos Colorectal Cancer. Clin. Can. Res. 10, 972-980.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PMS2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PMS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506658) Mouse IgG2b OTI2G5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PMS2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PMS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506683) Mouse IgG2b OTI4B7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PMS2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PMS2 Rabbit Polyclonal antibody. (TA590795) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PMS2 antibody

Western blot analysis of extracts from HeLa cells, using PMS2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PMS2 (58-81) antibody

WB analysis using Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. The blot was incubated with a 1:1,000 dilution of the antibody at RTfollowed by washing. A 1:20,000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemical's SuperSignal™ substrate. Comparison to a molecular weight marker (not shown) indicates a single band of ~96.0 kDa corresponding to the expected molecular weight for human PMS2 protein. Mouse IgG1 349.29.5.2 Purified Chimp, Hst, Hu, Ms, Rt E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PMS2 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human liver and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using PMS2 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PMS2 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human brain and ovarian cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using PMS2 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PMS2 antibody

Western Blot: PMS2 Antibody (163C1251) [TA336401] - Analysis of PMS2 using this antibody at 2 ug/ml against 10 ug of NIH 3T3 cell lysate. Mouse IgG1 163C1251 Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PMS2 (623-639) antibody

AM08355PU-N at 5 µg/ml staining PMS2 in Human Breast by Immunohistochemistry, Formalin-Fixed Paraffin-Embedded tissue. Mouse IgG1 163C1251 Purified Hu, Prim P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PMS2 Control Peptide

PMS2 Purified
50 µg / €190.00
  Acris Antibodies GmbH

PMS2 antibody

PMS2 Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

PMS2 antibody

Western blot (WB) analysis of PMS2 antibody (Cat.-No.: AP01248PU-N) in extracts from HeLa cells. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PMS2 (C-term) antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE). Rabbit IgG Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

PMS2 antibody

Immunofluorescence of purified MaxPab antibody to PMS2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

PMS2 antibody

PMS2 MaxPab rabbit polyclonal antibody. Western Blot analysis of PMS2 expression in human placenta. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PMS2 antibody

Detection limit for recombinant GST tagged PMS2 is approximately 3ng/ml as a capture antibody. Mouse IgG2a 4G4 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PMS2 antibody

PMS2 Mouse IgG2a 4A8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PMS2 antibody

PMS2 Mouse IgG2b 4D10 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PMS2 antibody

PMS2 Mouse IgG2b 4D11 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PMS2 antibody

Western blot analysis is shown using PMS2 monoclonal antibody, clone 349.29.5.2 ( Cat # MAB2089 ) to detect human PMS2 protein present in H157 cell lysates.
Approximately 5, 10 and 30 µg of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer.
A single band of ~96 kDa corresponding to the expected molecular weight for human PMS2 protein.
Personal communication Morphotek Inc. Mouse IgG1 349.29.5.2 Chimp, Hu, Ms, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

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