PNKP antibody

Principal name

PNKP antibody

Alternative names for PNKP antibody

Bifunctional polynucleotide phosphatase/kinase, Polynucleotide kinase-3'-phosphatase, DNA 5'-kinase/3'-phosphatase

SwissProt ID

Q96T60 (Human), Q9JLV6 (Mouse)

Gene ID

11284 (PNKP)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chimp (Chimpanzee), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of PNKP antibody

Kit Component:
- KN207551G1, PNKP gRNA vector 1 in pCas-Guide vector
- KN207551G2, PNKP gRNA vector 2 in pCas-Guide vector
- KN207551D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Jilani A, Ramotar D, Slack C, Ong C, Yang XM, Scherer SW, Lasko DD.
Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage.
J Biol Chem. 1999 Aug 20;274(34):24176-86.
PMID: 10446192

11 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PNKP antibody

  Rabbit polyclonal anti-PNK antibody  
WB using Anti-PNK antibody shows detection of a 57 kDa band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5). Approximately 10ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody. Rabbit IgG Aff - Purified Chimp, Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

PNKP antibody

  Rabbit Polyclonal Anti-PNKP Antibody  
WB Suggested Anti-PNKP Antibody; Titration: 1.0 ug/ml; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PNKP antibody

  Rabbit Polyclonal Anti-PNKP Antibody  
WB Suggested Anti-PNKP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PNKP (Internal) antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Kidney. Rabbit Aff - Purified Can, Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PNKP (C-term) antibody

PNKP Goat Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PNKP - human gene knockout kit via CRISPR

PNKP - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PNKP - human gene knockout kit via CRISPR

PNKP - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PNKP - human gene knockout kit via CRISPR

PNKP - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PNKP - human gene knockout kit via CRISPR

PNKP - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PNKP

  Recombinant protein of human polynucleotide kinase 3'-phosphatase (PNKP)  
PNKP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

PNKP overexpression lysate

PNKP overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

11 Item(s)

per page
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