PNKP antibody

Principal name

PNKP antibody

Alternative names for PNKP antibody

Bifunctional polynucleotide phosphatase/kinase, Polynucleotide kinase-3'-phosphatase, DNA 5'-kinase/3'-phosphatase

SwissProt ID

Q96T60 (Human), Q9JLV6 (Mouse)

Gene ID

11284 (PNKP)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chimp (Chimpanzee), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of PNKP antibody

We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. Mammalian polynucleotide kinase (PNK) is a key component of both the base excision repair (BER) and nonhomologous end-joining (NHEJ) DNA repair pathways. PNK acts as a 5'-kinase/3'-phosphatase to create 5'-phosphate/3'-hydroxyl termini, which are a necessary prerequisite for ligation during repair. PNK is recruited to repair complexes through interactions between its N-terminal FHA domain and phosphorylated components of either pathway.

General readings

Jilani A, Ramotar D, Slack C, Ong C, Yang XM, Scherer SW, Lasko DD.
Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage.
J Biol Chem. 1999 Aug 20;274(34):24176-86.
PMID: 10446192

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PNKP antibody

WB using Anti-PNK antibody shows detection of a 57 kDa band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5). Approximately 10ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody. Rabbit IgG Aff - Purified Chimp, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PNKP antibody

WB Suggested Anti-PNKP Antibody; Titration: 1.0 ug/ml; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PNKP antibody

WB Suggested Anti-PNKP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PNKP (Internal) antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Kidney. Rabbit Aff - Purified Can, Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PNKP (C-term) antibody

PNKP Goat Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

PNKP antibody

PNKP polyclonal antibody (A01), Lot # 051017JC01. Western Blot analysis of PNKP expression in Raw 264.7. Mouse Serum Hu, Ms, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PNKP antibody

PNKP MaxPab polyclonal antibody. Western Blot analysis of PNKP expression in human spleen. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PNKP antibody

PNKP Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PNKP antibody

Western blot using PNKP polyclonal antibody ( Cat # PAB11245 ) shows detection of a 57 kDa band corresponding to :
Lane 1, human PNKP in a Y190 yeast cell lysate.
Lane 2, Y190 yeast cell lysate + human PNKP ( Gal DNA BP ).
Lane 3, EM9 XH Chinese hamster ovary cell lysate.
Lane 4, EM9 XH Chinese hamster ovary cell lysate + human PNK.
Lane 5, a HeLa cell lysate.
Approximately 10 µg of lysate was run on SDS-PAGE and reaction with a 1:1000 dilution of PNKP polyclonal antibody. Rabbit Chimp, Hu, Ms, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

PNKP (N-term) antibody

Transfected 293T; WB Suggested Anti-PNKP Antibody. . Titration: 1.0 ug/ml. . Positive Control: Transfected 293T; PNKP antibody - N-terminal region (ARP52311_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Hu
50 µg / €350.00
  AVIVA Systems Biology

PNKP (Center) antibody

Transfected 293T; WB Suggested Anti-PNKP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; PNKP antibody - middle region (ARP52312_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PNKP antibody

Western Blot: PNK1 Antibodies SuperNovus Pack [NB100-927] - Detection of Human PNK1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody: NB100-550 used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 20 seconds. Rabbit Aff - Purified Hu IP, WB
3 vials / €760.00
  Novus Biologicals Inc.

PNKP antibody

PNKP Goat IgG Aff - Purified Hu E
0.1 mg / €400.00
  Novus Biologicals Inc.

PNKP antibody

PNKP Rabbit Aff - Purified Can, Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €480.00
  Novus Biologicals Inc.

PNKP antibody

PNK Antibody [NBP1-32764] - Immunohistochemical analysis of paraffin-embedded A549 xenograft , using PNK antibody at 1:500 dilution. Rabbit IgG Hu P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

PNKP antibody

Immunohistochemistry-Paraffin: PNK Antibody [NBP1-87257] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PNKP

PNKP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

PNKP

PNKP Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

PNKP

PNKP Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

PNKP

PNKP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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