PNPLA2 / ATGL antibody

Principal name

PNPLA2 / ATGL antibody

Alternative names for PNPLA2 / ATGL antibody

FP17548, Desnutrin, TTS2.2, IPLA2-zeta, Patatin-like phospholipase domain-containing protein 2, Adipose triglyceride lipase, Transport-secretion protein 2, Calcium-independent phospholipase A2, Pigment epithelium-derived factor

SwissProt ID

P0C548 (Rat), Q2KI18 (Bovin), Q8BJ56 (Mouse), Q96AD5 (Human)

Gene ID

57104 (PNPLA2), 66853 (Pnpla2)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Por (Porcine), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP)

Background of PNPLA2 / ATGL antibody

Lipolytic enzymes are required for mobilization of fatty acids from triglyceride stores in adipose tissue. Energy homeostasis is affected by dysfunctional lipolysis and may contribute to the pathogenesis of obesity and insulin resistance. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. It is now thought that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.

General readings

Schoenborn V, Heid IM, Vollmert C, Lingenhel A, Adams TD, Hopkins PN, Illig T, Zimmermann R, Zechner R, Hunt SC, Kronenberg F.
The ATGL gene is associated with free fatty acids, triglycerides, and type 2 diabetes.
Diabetes. 2006 May;55(5):1270-5.
PMID: 16644682

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PNPLA2 / ATGL antibody

Detection of ATGL in human adipose tissue lysate Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PNPLA2 / ATGL antibody

TA302576 (0.2µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PNPLA2 / ATGL antibody

TA302577 (0.03µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PNPLA2 / ATGL antibody

TA305683 (0.5ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PNPLA2 / ATGL antibody

Immunohistochemistry: Paraffin embedded sections of human breast cancer (left) and prostatic cancer (right) tissue were incubated with anti-human ATGL Cat.-No. AM09064PU (1:25) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer. Diaminobenzidine (DAB) was used for detection Mouse IgG2b AT2G2 Purified Hu, Ms E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

PNPLA2 / ATGL antibody

Immunohistochemistry: Paraffin embedded sections of human breast cancer (left) and prostatic cancer (right) tissue were incubated with anti-human ATGL Cat.-No. AM09064PU (1:25) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer. Diaminobenzidine (DAB) was used for detection Mouse IgG2b AT2G2 Purified Hu, Ms E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

PNPLA2 / ATGL antibody

AP08794PU-N at 2.5 µg/ml, staining PNPLA2 in Heart tissue by Immunohistochemistry (Formalin-Fixed Paraffin-Embedded). Goat IgG Aff - Purified Hu, Ms, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

PNPLA2 / ATGL antibody

PNPLA2 / ATGL Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PNPLA2 / ATGL antibody

Western Blot analysis of PNPLA2 expression in transfected 293T cell line (H00057104-T01) by PNPLA2 MaxPab polyclonal antibody.

Lane 1: PNPLA2 transfected lysate(55.44 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PNPLA2 / ATGL antibody

Western Blot analysis of PNPLA2 expression in transfected 293T cell line (H00057104-T01) by PNPLA2 MaxPab polyclonal antibody.

Lane 1: PNPLA2 transfected lysate(55.30 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PNPLA2 / ATGL antibody

Detection limit for recombinant GST tagged PNPLA2 is approximately 3ng/ml as a capture antibody. Mouse IgG2a 2H1 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PNPLA2 / ATGL antibody

Paraffin embedded sections of human breast ( A ) and prostatic cancer tissue ( B ) were incubated with PNPLA2 monoclonal antibody, clone AT2G2 ( Cat # MAB3210 ; 1 : 25)  for 2 hours at room temperature.
Antigen retrieval was performed in 100 mM sodium citrate buffer and detected using Diaminobenzidine ( DAB ). Mouse IgG2b AT2G2 Hu E, P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

PNPLA2 / ATGL antibody

PNPLA2 / ATGL Rabbit Ms E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

PNPLA2 / ATGL antibody

PNPLA2 / ATGL Goat Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

PNPLA2 / ATGL antibody

PNPLA2 polyclonal antibody ( Cat # PAB7589 ) ( 0.2 µg/ml ) staining of human adipose lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PNPLA2 / ATGL antibody

PNPLA2 polyclonal antibody ( Cat # PAB7590 ) ( 0.03 µg/ml ) staining of human heart lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PNPLA2 / ATGL antibody

Western blot analysis of PNPLA2 expression in rat kidney tissue lysate. Rabbit Hu, Ms, Rt C, IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PNPLA2 / ATGL antibody

Western blot analysis of PNPLA2 in human adipose tissue lysate with PNPLA2 polyclonal antibody ( Cat# PAB12069 ). Rabbit Hu, Ms WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

PNPLA2 / ATGL (C-term) antibody

Detection of Gpihbp1 in transfected lysate (Lane 1). An empty vector lysate was used as a negative control (Lane 2). Rabbit IgG Aff - Purified Hu WB
0.1 ml / €330.00
  Neuromics Antibodies

PNPLA2 / ATGL antibody

ATGL / Desnutrin Antibody[NB100-57854]-(2.5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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