PNPLA2 / ATGL antibody

Principal name

PNPLA2 / ATGL antibody

Alternative names for PNPLA2 / ATGL antibody

FP17548, Desnutrin, TTS2.2, IPLA2-zeta, Patatin-like phospholipase domain-containing protein 2, Adipose triglyceride lipase, Transport-secretion protein 2, Calcium-independent phospholipase A2, Pigment epithelium-derived factor

SwissProt ID

P0C548 (Rat), Q2KI18 (Bovin), Q8BJ56 (Mouse), Q96AD5 (Human)

Gene ID

57104 (PNPLA2), 66853 (Pnpla2), 361676

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)

Background of PNPLA2 / ATGL antibody

Kit Component:
- KN313547G1, Pnpla2 gRNA vector 1 in pCas-Guide vector
- KN313547G2, Pnpla2 gRNA vector 2 in pCas-Guide vector
- KN313547D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Schoenborn V, Heid IM, Vollmert C, Lingenhel A, Adams TD, Hopkins PN, Illig T, Zimmermann R, Zechner R, Hunt SC, Kronenberg F.
The ATGL gene is associated with free fatty acids, triglycerides, and type 2 diabetes.
Diabetes. 2006 May;55(5):1270-5.
PMID: 16644682

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PNPLA2 / ATGL antibody

  Rabbit Polyclonal Antibody against ATGL  
Detection of ATGL in human adipose tissue lysate Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PNPLA2 / ATGL antibody

  Goat Polyclonal Antibody against PNPLA2 / ATGL  
TA302576 (0.2µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PNPLA2 / ATGL antibody

  Goat Polyclonal Antibody against PNPLA2 / Desnutrin  
TA302577 (0.03µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PNPLA2 / ATGL antibody

  Goat Anti-ATGL / Desnutrin Antibody  
TA305683 (0.5ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PNPLA2 / ATGL antibody

Immunohistochemistry: Paraffin embedded sections of human breast cancer (left) and prostatic cancer (right) tissue were incubated with anti-human ATGL Cat.-No. AM09064PU (1:25) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer. Diaminobenzidine (DAB) was used for detection Mouse IgG2b AT2G2 Purified Hu, Ms E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

PNPLA2 / ATGL antibody

Immunohistochemistry: Paraffin embedded sections of human breast cancer (left) and prostatic cancer (right) tissue were incubated with anti-human ATGL Cat.-No. AM09064PU (1:25) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer. Diaminobenzidine (DAB) was used for detection Mouse IgG2b AT2G2 Purified Hu, Ms E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

PNPLA2 / ATGL antibody

AP08794PU-N at 2.5 µg/ml, staining PNPLA2 in Heart tissue by Immunohistochemistry (Formalin-Fixed Paraffin-Embedded). Goat IgG Aff - Purified Bov, Eq, Gt, Hst, Hu, Mky, Ms, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

PNPLA2 / ATGL (C-term) antibody

Detection of Gpihbp1 in transfected lysate (Lane 1). An empty vector lysate was used as a negative control (Lane 2). Rabbit IgG Aff - Purified Hu WB
0.1 ml / €330.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

PNPLA2 - human gene knockout kit via CRISPR

PNPLA2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PNPLA2 - human gene knockout kit via CRISPR

PNPLA2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PNPLA2 - human gene knockout kit via CRISPR

PNPLA2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PNPLA2 - human gene knockout kit via CRISPR

PNPLA2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pnpla2 - mouse gene knockout kit via CRISPR

Pnpla2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pnpla2 - mouse gene knockout kit via CRISPR

Pnpla2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pnpla2 - mouse gene knockout kit via CRISPR

Pnpla2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pnpla2 - mouse gene knockout kit via CRISPR

Pnpla2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PNPLA2 / ATGL

  Recombinant protein of human patatin-like phospholipase domain containing 2 (PNPLA2)  
PNPLA2 / ATGL Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PNPLA2 overexpression lysate

PNPLA2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PNPLA2 (mGFP-tagged) - Human patatin-like phospholipase domain containing 2 (PNPLA2), 200 uL, >10^7 TU/mL

Lenti ORF particles, PNPLA2 (mGFP-tagged) - Human patatin-like phospholipase domain containing 2 (PNPLA2), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PNPLA2 (Myc-DDK tagged) - Human patatin-like phospholipase domain containing 2 (PNPLA2) , 200 uL, >10^7 TU/mL

Lenti ORF particles, PNPLA2 (Myc-DDK tagged) - Human patatin-like phospholipase domain containing 2 (PNPLA2) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

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