Podocalyxin / PODXL antibody

Principal name

Podocalyxin / PODXL antibody

Alternative names for Podocalyxin / PODXL antibody

Podocalyxin-like protein 1, PCLP, PCLP1, PCLP-1, GCTM-2 antigen, Hematopoietic Stem Cell Marker

SwissProt ID

O00592 (Human), Q28645 (Rabit), Q9R0M4 (Mouse), Q9WTQ2 (Rat)

Gene ID

5420 (PODXL), 192181 (Podxl), 27205

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit), Can (Canine)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F), Enzyme Immunoassay (E), Immunofluorescence (IF)

Background of Podocalyxin / PODXL antibody

Podocalyxin, also known as podocalyxin-like or PDXL, is a member of the CD34 transmembrane sialomucin family. Like other sialoglycoproteins, podocalyxin plays important roles in mediating cellular migration and cell-cell adhesion. Podocalyxin was first discovered in podocytes, the terminally differentiated epithelial cells lining the kidney glomeruli. Podocalyxin is expressed at high levels on the podocyte foot projections and plays essential roles in kidney development and homeostasis, particularly blood filtration and urine formation.
Podocalyxin is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons (reviewed in Nielsen, 2009). Aberrant expression of podocalyxin has been described in a variety of cancers including breast, prostate, liver, pancreatic, kidney and leukemia. Human podocalyxin migrates as a 165/170 kDa doublet on SDS-PAGE; however there are many post-translational modifications which may impact its actual size (Kershaw, 1997).
Antibody to podocalyxin has been reported to be a useful biomarker for various pathological conditions. For example, podocalyxin antibody can identify podocytes in the urine, also known as podocyturia, which can be indicative of glomerular disease, pre-eclampsia, and other kidney pathology (reviewed in Chen, 2012). Additionally, podocalyxin antibody studies have indicated that highly podocalyxin-positive tumors or podocalyxin dysregulation in general can be potentially linked to more aggressive tumors and poorer outcomes (Nielson, 2009). Hence, podocalyxin antibody may be used as part of a biomarker antibody panel to study expression of proteins associated with tumorigenesis.

General readings

Nielsen JS, Graves ML, Chelliah S, Vogl AW, Roskelley CD, McNagny KM. The CD34-Related Molecule Podocalyxin Is a Potent Inducer of Microvillus Formation. PLoS ONE. 2007 Feb 21;2:e237. PMID: 17311105

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Podocalyxin / PODXL antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PODXL mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807749)(1:150) Mouse IgG1 OTI8D6 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Podocalyxin / PODXL antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PODXL mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807750)(1:150) Mouse IgG1 OTI2C6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Podocalyxin / PODXL antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney tissues (right) using PODXL mouse mAb with DAB staining. Mouse IgG1 5F5 Ascites Hu E, F, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Podocalyxin / PODXL antibody

Formalin-Fixed, Paraffin-Embedded Human angiosarcoma stained with Podocalyxin Antibody Cat.-No AM32857PU (Clone 3D3). Mouse IgG1 3D3 Purified Hu, Rb, Rt C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Podocalyxin / PODXL antibody

Formalin-Fixed, Paraffin-Embedded Human angiosarcoma stained with Podocalyxin Antibody Cat.-No AM32857PU (Clone 3D3). Mouse IgG1 3D3 Purified Hu, Rb, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Podocalyxin / PODXL antibody

Formalin-Fixed, Paraffin-Embedded Human angiosarcoma stained with Podocalyxin Antibody Cat.-No AM32857PU (Clone 3D3). Mouse IgG1 3D3 Purified Hu, Rb, Rt C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Podocalyxin / PODXL antibody

Formalin-Paraffin angiosarcoma stained with Podocalyxin Antibody Cat.-No AM33132SU (Clone 4F10). Mouse IgM 4F10 Supernatant Hu, Rb, Rt C, F, ICC/IF, P, WB
1.0 ml / €360.00
  Acris Antibodies GmbH

Podocalyxin / PODXL antibody

Formalin-Paraffin angiosarcoma stained with Podocalyxin Antibody Cat.-No AM33132SU (Clone 4F10). Mouse IgM 4F10 Supernatant Hu, Rb, Rt C, F, ICC/IF, P, WB
0.5 ml / €290.00
  Acris Antibodies GmbH

Podocalyxin / PODXL antibody

Formalin-Paraffin angiosarcoma stained with Podocalyxin Antibody Cat.-No AM33132SU (Clone 4F10). Mouse IgM 4F10 Supernatant Hu, Rb, Rt C, F, ICC/IF, P, WB
0.1 ml / €150.00
  Acris Antibodies GmbH

Podocalyxin / PODXL antibody

Formalin-paraffin angiosarcoma stained with Podocalyxin Antibody Cat.-No AM33133SU (Clone 2A4). Mouse IgM 2A4 Supernatant Hu, Rb, Rt C, F, ICC/IF, P, WB
1.0 ml / €360.00
  Acris Antibodies GmbH

Podocalyxin / PODXL antibody

Formalin-paraffin angiosarcoma stained with Podocalyxin Antibody Cat.-No AM33133SU (Clone 2A4). Mouse IgM 2A4 Supernatant Hu, Rb, Rt C, F, ICC/IF, P, WB
0.5 ml / €290.00
  Acris Antibodies GmbH

Podocalyxin / PODXL antibody

Formalin-paraffin angiosarcoma stained with Podocalyxin Antibody Cat.-No AM33133SU (Clone 2A4). Mouse IgM 2A4 Supernatant Hu, Rb, Rt C, F, ICC/IF, P, WB
0.1 ml / €150.00
  Acris Antibodies GmbH

Podocalyxin / PODXL antibody

Western blot analysis in NTERA-2 cl.D1 whole cell lysate Mouse IgM TRA-1-60(R) Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Podocalyxin / PODXL antibody

Western blot analysis of expression in NTERA-2 cl.D1 whole cell lysate. Mouse IgM TRA-1-81 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Podocalyxin / PODXL antibody

TA302578 (0.5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Podocalyxin / PODXL antibody

Western Blot: PODXL Antibody - Detection of podocalyxin in human lung lysate Rabbit Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Podocalyxin / PODXL antibody

Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PODXL antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Podocalyxin / PODXL antibody

Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human brain and ovarian cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using PODXL antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Podocalyxin / PODXL antibody

WB Suggested Anti-PODXL Antibody Titration: 5.0 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Podocalyxin / PODXL antibody

WB Suggested Anti-PODXL Antibody Titration: 0.5 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

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