Podoplanin antibody

Principal name

Podoplanin antibody

Alternative names for Podoplanin antibody

Glycoprotein 36, PA2.26 antigen, T1-alpha, Aggrus, PDPN, GP36, PSEC0003, PSEC0025

SwissProt ID

Q62011 (Mouse), Q64294 (Rat), Q86YL7 (Human), Q86YL7-3, Q95152 (Canfa)

Gene ID

10630 (PDPN), 14726 (Pdpn), 54320

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Hamster, Rabbit, Rat, Goat

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)

Background of Podoplanin antibody

Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

General readings

General / background references:Schacht V et al. T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema. EMBO J 22:3546-56 (2003). PubMed PMID: 12853470
Zimmer G et al. Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium. Biochem J 341 ( Pt 2):277-84 (1999). PubMed PMID: 10393083
Breiteneder-Geleff S et al. Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis. Am J Pathol 151:1141-52 (1997). PubMed PMID: 9327748

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Podoplanin antibody

DM3500 Podoplanin antibody staining of Paraffin Embedded Human Mucosa Sections. Mouse IgG1 18H5 Supernatant Hu C, F, IP, P, WB
0.2 ml / €370.00
  Acris Antibodies GmbH

Podoplanin antibody

Immunofluorescence staining (green) of Podoplanin in primary human dermal lymphatic endothelial cells (HDLEC) with anti-Human Podoplanin Antibody VCat.-No DM3500P (0.5 μg/ml) and counter staining of nuclei with Dapi. As secondary antibody Goat anti-Mouse ALEXA Fluor 488 was used 1/400. Mouse IgG1 18H5 Aff - Purified Hu C, F, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Podoplanin antibody

NZ-1 Staining of Human Mesothelial Cells. Rat IgG2a NZ-1 Purified Hu C, F, IP, P, WB
100 µg / €370.00
  Acris Antibodies GmbH

Podoplanin antibody

Podoplanin Mouse IgG1 D2-40 Purified Hu C, ICC/IF, P, WB
1 ml / €610.00
  Acris Antibodies GmbH

Podoplanin antibody

FACS analysis with Biotinylated anti-Human Podoplanin antibody Cat.-No DM3500B using HDMVECs. Mouse IgG1 18H5 Biotin Hu F
50 µg / €330.00
  Acris Antibodies GmbH

Podoplanin antibody

FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC). Upper panel: no primary antibody; Middle panel: solely conjugated secondary antibody; Lower panel: FITC-conjugated anti-human Podoplanin antibody. Mouse IgG1 18H5 FITC Hu F
50 µg / €350.00
  Acris Antibodies GmbH

Podoplanin antibody

FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC). Upper panel: no primary antibody; Middle panel: solely conjugated secondary antibody; Lower panel: FITC-conjugated anti-human Podoplanin antibody. Mouse IgG1 18H5 FITC Hu F
25 µg / €240.00
  Acris Antibodies GmbH

Podoplanin antibody

FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC). Mouse IgG1 18H5 PE Hu F
50 µg / €350.00
  Acris Antibodies GmbH

Podoplanin antibody

FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC). Mouse IgG1 18H5 PE Hu F
25 µg / €240.00
  Acris Antibodies GmbH

Podoplanin antibody

Immunohistochemistry of Mouse Liver (paraffin embedded sections) using anti-Podoplanin antibody Hamster IgG 811 Purified Ms C, IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Podoplanin antibody

Western blot analysis using Podoplanin Antibody Cat.-No DM3516P with recombinant Human, Mouse and Rat soluble Podoplanin. There is no cross reaction with Human Podoplanin. Hamster IgG RTD4E10 Purified Ms C, IP, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Podoplanin antibody

Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis with the polyclonal (Cat.-No DP3512) antibodies. The antibody recognizes a protein of about 36 kDa in total lysate from LECs but not from HUVEC. Rabbit Azide Free Hu ICC/IF, IP, WB
0.2 mg / €370.00
  Acris Antibodies GmbH

Podoplanin antibody

Podoplanin Rabbit Azide Free Hu ICC/IF, IP, WB
0.1 mg / €270.00
  Acris Antibodies GmbH

Podoplanin (38-51) antibody

Immunohistochemical detection of Human Aggrus/Podoplanin on paraffin embedded section of Human normal rectum with AM26512SU-N. Rat IgG2a YM-1 Supernatant Hu F, P, WB
0.1 ml / €390.00
  Acris Antibodies GmbH

Podoplanin antibody

Immunohistochemical detection (whole mount) of mouse Aggrus (Podoplanin) on paraffin embedded section of mouse normal lung with AM26513AF-N. Rat IgG2a 8F11 Azide Free Ms F, IP, P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

Podoplanin antibody

Staining of lymphatic endothelial cells and podocytes in normal Rat renal corpuscle (Paraffin-Embedded Tissue-Sections fixed in Formalin) with Podoplanin Antibody Cat.-No. DM3614P (Clone LF3(B7)D5B3) Mouse IgG1 LF3(B7)D5B3 Purified Rt C, ICC/IF, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

Podoplanin antibody

TA305702 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Podoplanin antibody

Immunohistochemistry staining of Paraffin Tonsil tissue by Podoplanin antibody (dilution: 1:20 - 1:100; visualization of staining: Cytoplasmic) Mouse IgG1 D2-40 Purified Hu P
1 ml / €600.00
  OriGene Technologies, Inc.

Podoplanin antibody

WB Suggested Anti-PDPN Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Podoplanin antibody

WB Suggested Anti-PDPN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Placenta Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

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