POFUT1 antibody

Principal name

POFUT1 antibody

Alternative names for POFUT1 antibody

FUT12, O-FucT-1, KIAA0180, GDP-fucose protein O-fucosyltransferase 1, Peptide-O-fucosyltransferase 1

SwissProt ID

Q18014 (Caeel), Q6EV70 (Rat), Q91ZW2 (Mouse), Q9H488 (Human), Q9V6X7 (Drome)

Gene ID

23509 (POFUT1), 180607 (C15C7.7)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey), Por (Porcine), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of POFUT1 antibody

POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling. At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.

General readings

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Stahl, M., et al. J. Biol. Chem. 283(20):13638-13651(2008)
Luo, Y., et al. J. Biol. Chem. 280(12):11289-11294(2005)
Shi, S., et al. Proc. Natl. Acad. Sci. U.S.A. 100(9):5234-5239(2003)
Panin, V.M., et al. J. Biol. Chem. 277(33):29945-29952(2002)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POFUT1 antibody

Western blot analysis of POFUT1 in human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

POFUT1 antibody

Western blot analysis of extracts from HeLa cells, Jurkat cells and HepG2 cells, using POFUT1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POFUT1 antibody

WB Suggested Anti-POFUT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: PANC1 cell lysate POFUT1 is strongly supported by BioGPS gene expression data to be expressed in Human PANC1 cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

POFUT1 Control Peptide

POFUT1
50 µg / €160.00
  Acris Antibodies GmbH

POFUT1 (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue reacted with POFUT1 Antibody (C-term) Cat-No AP53375PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of POFUT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

POFUT1 antibody

POFUT1 MaxPab rabbit polyclonal antibody. Western Blot analysis of POFUT1 expression in mouse kidney. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

POFUT1 antibody

POFUT1 Mouse IgG2a 3H1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

POFUT1 antibody

Western blot analysis of POFUT1 in human heart tissue lysate with POFUT1 polyclonal antibody ( Cat # PAB13063 ) at ( A ) 0.5 and ( B ) 1 µg/ml. Rabbit Hu, Ms WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

POFUT1 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using POFUT1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

POFUT1 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using POFUT1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

POFUT1 antibody

Immunohistochemical analysis of POFUT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Can, Hu, Mky, Por ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

POFUT1 antibody

Immunohistochemical analysis of POFUT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Can, Hu, Mky, Por ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

POFUT1 antibody

Immunohistochemical analysis of POFUT1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Can, Hu, Mky, Por ICC/IF, IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

POFUT1 antibody

POFUT1 Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

POFUT1 antibody

POFUT1 Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €290.00
  Novus Biologicals Inc.

POFUT1 antibody

Immunohistochemistry-Paraffin: POFUT1 Antibody [NBP1-76940] - Human heart tissue with POFUT antibody at 2.5 ug/mL._x000d_ Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

POFUT1 (transcript variant 1)

POFUT1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

POFUT1 (His-tag)

POFUT1 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

POFUT1 (His-tag)

POFUT1 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

POFUT1

POFUT1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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