POLE3 antibody

Principal name

POLE3 antibody

Alternative names for POLE3 antibody

CHRAC17, DNA polymerase epsilon subunit 3, DNA polymerase II subunit 3, DNA polymerase epsilon subunit p17, Chromatin accessibility complex 17, Arsenic-transactivated protein

SwissProt ID

Q3SZN5 (Bovin), Q642A5 (Rat), Q9JKP7 (Mouse), Q9NRF9 (Human)

Gene ID

54107 (POLE3), 5426 (POLE)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Mky (Monkey), Hst (Hamster), Mink, Dros (Drosophila), Md (Mustelid), Prim (Primate), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), EPair for Elisa (EPair)

Background of POLE3 antibody

DNA polymerase epsilon from HeLa cells differs from yeast pol epsilon in that HeLa pol epsilon contains two subunits of 220 and 55 kDa, whereas yeast pol epsilon has one large subunit and three smaller subunits. Previous studies of template specificity of HeLa pol epsilon, which appears to be required for long patch DNA repair in permeabilized cells, indicate pol epsilon prefers long stretches of single-stranded regions in poly(dA)-oligo(dT) as template. HeLa pol alpha and pol delta prefer activated DNA and alternating poly(dA-dT) as templates, respectively. Pol epsilon retains its processivity in the absence of proliferating cell nuclear antigen (PCNA), unlike pol delta. PCNA can also interact with Pol epsilon on linear DNA templates, even in the absence of other auxiliary factors (replication factor C, replication factor A), and thereby stimulate its primer recognition and DNA synthesis.

General readings

Bolognese F, Imbriano C, et al. (2000). Nucleic Acids Res. 28(19):3830-8.Li Y, Pursell ZF, et al. (2000). J Biol Chem. 275(30):23247-52.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POLE3 antibody

Western Blot: DNA polymerase epsilon Antibody (3C5.1) - SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction of HeLa DNA pol (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver. Mouse IgG2a 3C5.1 Purified Dros, Hu, Ms, Prim WB
50 µl / €325.00
  OriGene Technologies, Inc.

POLE3 antibody

POLE3 Antibody (N-term) (Cat. #TA324920) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POLE3 antibody detected the POLE3 protein (arrow). Rabbit IgG Purified Bov, Hu, Ms, Rt F, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

POLE3 antibody

WB Suggested Anti-POLE3 Antibody Titration: 2.5ug/ml; Positive Control: Daudi cell lysatePOLE3 is supported by BioGPS gene expression data to be expressed in Daudi Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

POLE3 antibody

WB Suggested Anti-POLE3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Lung Rabbit IgG Purified Can, Ms, Rb, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

POLE3 antibody

POLE3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

POLE3 antibody

Immunofluorescence of purified MaxPab antibody to POLE3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

POLE3 antibody

Immunofluorescence of purified MaxPab antibody to POLE3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

POLE3 antibody

Immunofluorescence of purified MaxPab antibody to POLE3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

POLE3 antibody

Western Blot analysis of POLE3 expression in transfected 293T cell line (H00054107-T02) by POLE3 MaxPab polyclonal antibody.

Lane 1: POLE3 transfected lysate(16.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

POLE3 antibody

Western blot analysis of POLE3 in HeLa cell lysate with POLE3 monoclonal antibody ( Cat # MAB2307 ). Mouse IgG2a Hu, Ms WB
50 µl / €470.00
  Abnova Taiwan Corp.

POLE3 antibody

Human Daudi; WB Suggested Anti-POLE3 Antibody Titration: 2.5ug/ml. Positive Control: Daudi cell lysate. POLE3 is supported by BioGPS gene expression data to be expressed in Daudi.; POLE3 antibody - N-terminal region (ARP33054_T100) in Human Daudi cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

POLE3 antibody

Human Lung; WB Suggested Anti-POLE3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Lung; POLE3 antibody - N-terminal region (ARP58048_P050) in Human Lung cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

POLE3 antibody

POLE3 Mouse IgG2a 3C5.1 Purified Hu, Md, Mky, Ms ICC/IF, IP, WB
25 µl / €220.00
  Novus Biologicals Inc.

POLE3 antibody

Immunohistochemistry-Paraffin: DNA Polymerase epsilon Antibody [NB100-60438] - FFPE section of human breast carcinoma.   Affinity purified rabbit anti-PolE3/p17 1:100.  Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit Aff - Purified Hu IP, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

POLE3

POLE3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

POLE3 (1-147, His-tag)

POLE3 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

POLE3 (1-147, His-tag)

POLE3 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

POLE3

POLE3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

POLE3

POLE3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

POLE3 (Human) Matched Antibody Pair

POLE3 (Human) Matched Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

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