POLH / RAD30 antibody

Principal name

POLH / RAD30 antibody

Alternative names for POLH / RAD30 antibody

DNA polymerase eta, RAD30A, XPV, Xeroderma pigmentosum variant type protein, DBH1, Radiation-sensitive protein 30

SwissProt ID

Q04049 (Yeast), Q9JJN0 (Mouse), Q9Y253 (Human)

Gene ID

5429 (POLH), 852028 (RAD30)

Available reactivities

Hu (Human), Ye (Yeast), S. cerevisiae (Saccharomyces cerevisiae), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of POLH / RAD30 antibody

Saccharomyces cerevisiae Radiation sensitive protein 30 (Rad30) is the homolog of human DNA polymerase eta. The Saccharomyces cerevisiae RAD30 gene, a homologue of Escherichia coli dinB and umuC, is DNA damage inducible and functions in a novel error-free postreplication repair mechanism. Both human and yeast polymerase eta are responsible for error-free bypass of UV-induced cis-syn pyrimidine dimers and several other DNA lesions. The RAD30A gene encodes DNA polymerase eta and defects in the protein lead to the xeroderma pigmentosum variant (XP-V) phenotype in humans. The hypermutations in all 4 bases in immunoglobulin genes are the result of low fidelity DNA polymerases like Poleta encoded by Rad30 in S. cerevisea, RAD 30 is post-transalationally modified by Ubiquitinaion and proteosome mediated degradation. The biological half life for Rad30 is 20 minutes and is increased due ot proteosome defects (1). Other mutation inactivating proteins like Skp and F-box protein Ufo1 stabilize Rad30. UV radiation causes transient stabilization of Rad30 which tend to accumulate this protein in the cells. DNA polymerase eta, involved in the predominantly error-free bypass replication of DNA lesions, catalyzes the efficient and accurate synthesis of DNA opposite cyclobutane pyrimidine dimers; homolog of human POLH and bacterial DinB proteins.
The Rad30 gene resides on S cerevisea chromosome 4. There are wo variants of Rad30 proteins, Rad30-101AP antibodies were generated agisnt 632 amino acid protein from sacchromyces cerevisea. Rad30 contain several functional domain, including UmuC (26-309); Pol30 binding domain (625-632) and required Mg for its activity. Mutations at Aspartic acid 30 and or at 155, to Alanine abolished the DNA polymerse activity and several other mutations alter the enzymatic acitivity in different fashion.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POLH / RAD30 antibody

Western blot analysis of extracts of various cell lines , using POLH antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

POLH / RAD30 antibody

Predicted band size: 78 kDa . Positive control: A431 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POLH / RAD30 antibody

POLH Antibody (N-term) (Cat.# TA328145) western blot analysis in CEM cell lysate (35ug/lane). This demonstrates that the POLH antibody detected the POLH protein (arrow). Rabbit RB40403 Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

POLH / RAD30 control peptide

POLH / RAD30
0.1 mg / €250.00
  Acris Antibodies GmbH

POLH / RAD30 (284-300) antibody

POLH / RAD30 Rabbit IgG Aff - Purified S. cerevisiae WB
0.1 mg / €420.00
  Acris Antibodies GmbH

POLH / RAD30 antibody

Immunofluorescence of purified MaxPab antibody to POLH on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

POLH / RAD30 antibody

POLH / RAD30 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

POLH / RAD30 antibody

Western Blot analysis of POLH expression in transfected 293T cell line (H00005429-T02) by POLH MaxPab polyclonal antibody.

Lane 1: POLH transfected lysate(78.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

POLH / RAD30 antibody

POLH / RAD30 Rabbit Ye WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

POLH / RAD30 antibody

Western Blot: PolH Antibody [NB100-60423] - Detection of Human PolH by Western Blot of Immunoprecipitates.  Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PolH antibody NB100-60423 used for IP at 3 mcg/mg lysate. PolH was also immunoprecipitated by rabbit anti-PolH antibody NB100-60424, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

POLH / RAD30 antibody

Western Blot and Immunoprecipitation: POLH Antibody [NB100-60424] - Detection of Human PolH on HeLa whole cell lysate using NB100-60424. PolH was also immunoprecipitated by rabbit anti-PolH antibody NB100-60423. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

POLH / RAD30 antibody

Immunohistochemistry-Paraffin: DNA polymerase eta Antibody [NBP1-87165] - Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

POLH / RAD30 antibody

Immunohistochemistry-Paraffin: DNA polymerase eta Antibody [NBP1-87166] - Immunohistochemical staining of human prostate shows moderate nuclear and cytoplasmic positivity in basal cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

POLH / RAD30

POLH / RAD30 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

POLH / RAD30

POLH / RAD30 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

POLH / RAD30

POLH / RAD30 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

POLH (Human) IP-WB Antibody Pair

POLH (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

POLH 293T Cell Transient Overexpression Lysate(Denatured)

POLH 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

18 Item(s)

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