POLR2A antibody

Principal name

POLR2A antibody

Alternative names for POLR2A antibody

RNA polymerase II, RNA polymerase II subunit B1, POLR2, POLRA, RPOL2, RPB1, RPB220, DNA-directed RNA polymerase II subunit RPB1, RNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd

SwissProt ID

P04050 (Yeast), P04052 (Drome), P08775 (Mouse), P16356 (Caeel), P18616 (Arath), P24928 (Human), P36594 (Schpo)

Gene ID

851415 (RPO21), 5430 (POLR2A), 177190 (ama-1), 829734 (NRPB1)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Xen (Xenopus), Dros (Drosophila), Arabidopsis, Hst (Hamster), Pl (Plant), Ye (Yeast), Ce (C. elegans), Rt (Rat), Chk (Chicken), Mky (Monkey), S. cerevisiae (Saccharomyces cerevisiae), Can (Canine), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Rat

Available applications

ChIP assay (ChIP), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Flow Cytometry (F), Immunoprecipitation (IP), Paraffin Sections (P), Dot blot (Dot)

Background of POLR2A antibody

R polymerase II (RPII) is a large multi-protein complex that functions as a D-dependent R polymerase, catalyzing the transcription of D into R using the four ribonucleoside triphosphates as substrates. The largest subunit, RPII subunit B1 (Rpb1), also known as RPII subunit A (POLR2A), contains a unique heptapeptide sequence (Tyr1,Ser2,Pro3,Thr4,Ser5,Pro6,Ser7), which is repeated up to 52 times in the carboxy-termil domain (CTD) of the protein. This CTD heptapeptide repeat is subject to multiple post-translatiol modifications, which dictate the functiol state of the polymerase complex. Phosphorylation of the CTD during the active transcription cycle integrates transcription with chromatin remodeling and scent R processing by regulating the recruitment of chromatin modifying enzymes and R processing proteins to the transcribed gene. During transcription initiation, RPII contains a hypophosphorylated CTD and is recruited to gene promoters through interactions with D-bound transcription factors and the Mediator complex. The escape of RPII from gene promoters requires phosphorylation at Ser5 by CDK7, the catalytic subunit of transcription factor IIH (TFIIH). Phosphorylation at Ser5 mediates the recruitment of R capping enzymes, in addition to histone H3 Lys4 methyltransferases, which function to regulate transcription initiation and chromatin structure. After promoter escape, RPII proceeds down the gene to an intrinsic pause site, where it is halted by the negative elongation factors NELF and DSIF. At this point, RPII is unstable and frequently aborts transcription and dissociates from the gene. Productive transcription elongation requires phosphorylation at Ser2 by CDK9, the catalytic subunit of the positive transcription elongation factor P-TEFb. Phosphorylation at Ser2 creates a stable transcription elongation complex and facilitates recruitment of R splicing and polyadenylation factors, in addition to histone H3 Lys36 methyltransferases, which function to promote elongation-compatible chromatin.

General readings

This product has been used in:Patturajan M et al. Growth-related changes in phosphorylation of yeast RNA polymerase II. J Biol Chem 273:4689-94 (1998). PubMed PMID: 9468530
Feng Y et al. Fragile X mental retardation protein: nucleocytoplasmic shuttling and association with somatodendritic ribosomes. J Neurosci 17:1539-47 (1997). PubMed PMID: 9030614
Bellier S et al. Nuclear translocation and carboxyl-terminal domain phosphorylation of RNA polymerase II delineate the two phases of zygotic gene activation in mammalian embryos. EMBO J 16:6250-62 (1997). PubMed PMID: 9321404
Thompson NE et al. Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibody. J Biol Chem 265:7069-77 (1990). PubMed PMID: 2324114
Thompson NE et al. Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II. J Biol Chem 264:11511-20 (1989). PubMed PMID: 2472398

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POLR2A antibody

Western Blot: RNA polymerase II Antibody (4H8) - Western blot analysis on NIH/3T3 cell lysate using anti-RNA polymerase II. Mouse IgG1 4H8 Purified Hu, Ms, Ye E, ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POLR2A antibody

Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30min), using POLR2A (Ab-1619) antibody (#TA326076, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POLR2A antibody

Western blot analysis of extracts of HeLa cell lines, using POLR2A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

POLR2A antibody

Western Blot: RNA polymerase II [p Thr4] Antibody [TA336792] - Flag-tagged Rpb1 was IP'd with Flag antibody. Western blotting was performed using P-thr4 antibody. 26r: Rpb1 with 26 all-consensus YSPTSPS repeat CTD. T4V: Rpb1 with 30 YSPVSPS repeat CTD. Rabbit Purified Chk, Dros, Hu, Ye WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

POLR2A antibody

Host: Rabbit; Target Name: POLR2A; Sample Tissue: Hela whole cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

POLR2A antibody

WB using the antibody against Pol II diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Mouse IgG1 Monoclol Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

POLR2A antibody

WB using the antibody against Pol II S2p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Mouse IgG1 Monoclol Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

POLR2A antibody

WB using the antibody against Pol II S5p diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Mouse IgG1 Monoclol Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

POLR2A antibody

Immunohistochemistry analysis of POLR2A antibody (Cat.-No. AP06407PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

POLR2A (794-822) antibody

POLR2A Mouse IgG1 ARNA-3 Supernatant Dros, Hu C, ICC/IF, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

POLR2A antibody

POLR2A Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

POLR2A antibody

POLR2A Mouse IgG2b 1F17 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

POLR2A antibody

ChIP results obtained with the POLR2A monoclonal antibody ( Cat # MAB0120 ) . ChIP assays were performed using MCF-7 cells ( Breast carcinoma cell line ) treated with estradiol ( MCF-7 + E2 ) or not treated ( MCF-7 ) . Mouse IgG2a Hu ChIP
0.1 ml / €470.00
  Abnova Taiwan Corp.

POLR2A (phospho + non-phospho) antibody

POLR2A Mouse IgG1 4H8 Hst, Hu, Ms, Ye E, ICC/IF, IP, WB
50 µl / €470.00
  Abnova Taiwan Corp.

POLR2A pSer5 antibody

POLR2A Mouse IgM 8A7 Hu, Ye E, ICC/IF, WB
50 µl / €470.00
  Abnova Taiwan Corp.

POLR2A pSer1619 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

POLR2A pSer1619 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using POLR2A (Phospho-Ser1619) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

POLR2A antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using POLR2A (Ab-1619) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

POLR2A antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using POLR2A (Ab-1619) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

POLR2A pSerer5 antibody

Immunohistochemical analysis of Rpb1 CTD (pSer5) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Can, Hu, Ms, Rt, Ze ICC/IF, P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

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