POLR2E antibody

Principal name

POLR2E antibody

Alternative names for POLR2E antibody

RNA polymerases I, II, and III subunit ABC1, RPABC1, XAP4, DNA-directed RNA polymerases I, II, and III subunit RPABC1, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, RPB5 homolog

SwissProt ID

B0BNE2 (Rat), P19388 (Human), P20434 (Yeast), Q09191 (Schpo), Q80UW8 (Mouse), Q9N5K2 (Caeel)

Gene ID

5434 (POLR2E), 66420 (Polr2e)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of POLR2E antibody

Kit Component:
- KN201266G1, POLR2E gRNA vector 1 in pCas-Guide vector
- KN201266G2, POLR2E gRNA vector 2 in pCas-Guide vector
- KN201266D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Kershnar E., et al. (1998) J. Biol. Chem. 273:34444-34453Dorjsuren D., et al. (1998) Mol. Cell. Biol. 18:7546-7555

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  2. Highly Validated (2)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POLR2E antibody

  Rabbit anti-POLR2E Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using POLR2E antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

POLR2E antibody

  POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502548) Mouse IgG2a OTI3B5 Purified Can, Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

POLR2E antibody

  Rabbit polyclonal anti-RPAB1 antibody  
Western blot analysis of extracts from MCF-7 cells, using RPAB1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POLR2E antibody

  POLR2E mouse monoclonal antibody, clone OTI3C5 (formerly 3C5)  
Anti-POLR2E mouse monoclonal antibody (TA502488) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2E(RC201266). Mouse IgG2a OTI3C5 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POLR2E antibody

  POLR2E mouse monoclonal antibody, clone OTI2A11 (formerly 2A11)  
Anti-POLR2E mouse monoclonal antibody (TA502490) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2E(RC201266). Mouse IgG1 OTI2A11 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POLR2E antibody

Immunohistochemistry (IHC) analyzes of POLR2E antibody (Cat.-No.: AP21061PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

POLR2E antibody

Western Blot analysis of POLR2E expression in transfected 293T cell line (H00005434-T01) by POLR2E MaxPab polyclonal antibody.

Lane 1: POLR2E transfected lysate(23.1 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

POLR2E antibody

Immunoprecipitation of POLR2E transfected lysate using anti-POLR2E MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with POLR2E purified MaxPab mouse polyclonal antibody (B01P) (H00005434-B01P). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

POLR2E antibody

Western blot analysis of extracts from MCF-7 cells, using RPAB1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

POLR2E antibody

Western blot analysis of extracts from MCF-7 cells, using RPAB1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

POLR2E antibody

Immunohistochemical analysis of POLR2E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

POLR2E antibody

Immunohistochemical analysis of POLR2E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

POLR2E antibody

Immunohistochemical analysis of POLR2E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

POLR2E

  Recombinant protein of human polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (POLR2E)  
POLR2E Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

POLR2E (1-210, His-tag)

POLR2E Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

POLR2E (1-210, His-tag)

POLR2E Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

POLR2E

POLR2E Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

POLR2E

POLR2E Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

POLR2E (Human) IP-WB Antibody Pair

POLR2E (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

POLR2E 293T Cell Transient Overexpression Lysate(Denatured)

POLR2E 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

20 Item(s)

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