POLR2E antibody

Principal name

POLR2E antibody

Alternative names for POLR2E antibody

RNA polymerases I, II, and III subunit ABC1, RPABC1, XAP4, DNA-directed RNA polymerases I, II, and III subunit RPABC1, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, RPB5 homolog

SwissProt ID

B0BNE2 (Rat), P19388 (Human), P20434 (Yeast), Q09191 (Schpo), Q80UW8 (Mouse), Q9N5K2 (Caeel)

Gene ID

5434 (POLR2E)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of POLR2E antibody

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Kershnar E., et al. (1998) J. Biol. Chem. 273:34444-34453Dorjsuren D., et al. (1998) Mol. Cell. Biol. 18:7546-7555

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POLR2E antibody

Western blot analysis of extracts of various cell lines, using POLR2E antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

POLR2E antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502548) Mouse IgG2a OTI3B5 Purified Can, Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

POLR2E antibody

Western blot analysis of extracts from MCF-7 cells, using RPAB1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POLR2E antibody

Anti-POLR2E mouse monoclonal antibody (TA502488) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2E(RC201266). Mouse IgG2a OTI3C5 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POLR2E antibody

Anti-POLR2E mouse monoclonal antibody (TA502490) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2E(RC201266). Mouse IgG1 OTI2A11 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POLR2E antibody

Immunohistochemistry (IHC) analyzes of POLR2E antibody (Cat.-No.: AP21061PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

POLR2E antibody

Western Blot analysis of POLR2E expression in transfected 293T cell line (H00005434-T01) by POLR2E MaxPab polyclonal antibody.

Lane 1: POLR2E transfected lysate(23.1 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

POLR2E antibody

Immunoprecipitation of POLR2E transfected lysate using anti-POLR2E MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with POLR2E purified MaxPab mouse polyclonal antibody (B01P) (H00005434-B01P). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

POLR2E antibody

Western blot analysis of extracts from MCF-7 cells, using RPAB1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

POLR2E antibody

Western blot analysis of extracts from MCF-7 cells, using RPAB1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

POLR2E antibody

Immunohistochemical analysis of POLR2E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

POLR2E antibody

Immunohistochemical analysis of POLR2E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

POLR2E antibody

Immunohistochemical analysis of POLR2E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

POLR2E antibody

POLR2E Rabbit Hu, Ms, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

POLR2E

POLR2E Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

POLR2E (1-210, His-tag)

POLR2E Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

POLR2E (1-210, His-tag)

POLR2E Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

POLR2E

POLR2E Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

POLR2E

POLR2E Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

POLR2E (Human) IP-WB Antibody Pair

POLR2E (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

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