POLR2I antibody

Principal name

POLR2I antibody

Alternative names for POLR2I antibody

RNA polymerase II subunit B9, RPB9, RPB14.5, DNA-directed RNA polymerase II subunit RPB9, DNA-directed RNA polymerase II subunit I, RNA polymerase II 14.5 kDa subunit

SwissProt ID

O74635 (Schpo), P36954 (Human), P36958 (Drome), P60898 (Mouse), P60899 (Pig), Q32P73 (Bovin)

Gene ID

5438 (POLR2I), 69920 (Polr2i)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Chk (Chicken), Mky (Monkey), Rb (Rabbit), Eq (Equine), GP (Guinea Pig), Ye (Yeast), Gt (Goat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of POLR2I antibody

POLR2I is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. POLR2I has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Kershnar E., et al. (1998) J. Biol. Chem. 273:34444-34453

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POLR2I antibody

  Anti-POLR2I Rabbit Polyclonal Antibody  
Predicted band size: 15 kDa . Positive control: LO2 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POLR2I antibody

  POLR2I Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using POLR2I antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

POLR2I antibody

  Rabbit Polyclonal Anti-POLR2I Antibody  
WB Suggested Anti-POLR2I Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate POLR2I is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

POLR2I antibody

  Rabbit Polyclonal Anti-POLR2I Antibody  
WB Suggested Anti-POLR2I Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

Polr2i - mouse gene knockout kit via CRISPR

Polr2i - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Polr2i - mouse gene knockout kit via CRISPR

Polr2i - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Polr2i - mouse gene knockout kit via CRISPR

Polr2i - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Polr2i - mouse gene knockout kit via CRISPR

Polr2i - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

POLR2I (1-125, His-tag)

POLR2I Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

POLR2I (1-125, His-tag)

POLR2I Human Purified > 85 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, POLR2I (mGFP-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa (POLR2I), 200 uL, >10^7 TU/mL

Lenti ORF particles, POLR2I (mGFP-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa (POLR2I), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, POLR2I (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa (POLR2I), 200 uL, >10^7 TU/mL

Lenti ORF particles, POLR2I (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa (POLR2I), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Polr2i (GFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide I (Polr2i), 200 uL, >10^7 TU/mL

Lenti ORF particles, Polr2i (GFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide I (Polr2i), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Polr2i (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide I (Polr2i), 200 uL, >10^7 TU/mL

Lenti ORF particles, Polr2i (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide I (Polr2i), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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