POLR2J antibody

Principal name

POLR2J antibody

Alternative names for POLR2J antibody

RNA polymerase II subunit B11-a, POLR2J1, RPB11a, DNA-directed RNA polymerase II subunit RPB11-a, DNA-directed RNA polymerase II subunit RPB11-a, DNA-directed RNA polymerase II subunit J-1, RNA polymerase II 13.3 kDa subunit

SwissProt ID

O08740 (Mouse), P52435 (Human), Q32P79 (Bovin), Q9VJE4 (Drome)

Gene ID

5439 (POLR2J)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of POLR2J antibody

Four independent genes encoding various variants of the hRPB11 subunit of Homo sapiens RNA polymerase II were revealed in human chromosome 7. Three genes (POLR2 J1, POLR2 J2, and POLR2 J3) form a cluster of total length of 214 530 bp in the genetic locus 7q22.1 on the long arm of chromosome 7 (contig NT_007933). The fourth gene (POLR2 J4, 31 040 bp) was localized in the cytogenetic locus 7p13 of the short arm of chromosome 7 (contig NT_007819). An analysis enabled us to refine dissimilar experimental data on the mapping of the hRPB11 subunit gene on chromosome 7. In particular, the presence of three sites of its localization according to data on hybridization with fluorescent-labeled probes (the FISH method) was explained. It was established that, upon the expression of the four human POLR2 J genes, at least 14 types of mature mRNAs encoding somewhat differing hRPB11 isoforms can be synthesized. Eleven of these mRNAs were revealed (as full-length copies or clearly identifiable fragments) in the available databases of expressed sequence tags and cDNAs. The most probable scheme of origination of the multiple genes of the POLR2 J family as a result of three consecutive segmented duplications increasing in size was proposed and substantiated. On the basis of the scheme, some assumptions on the pathways of evolution of separate human genes and the mechanisms of generation of protein diversity in higher eukaryotes were made.

General readings

Proshkin,S.A., et al. (2011) Biochemistry Mosc. 76 (8), 976-980Shpakovskii,D.G., et al. (2004) Bioorg. Khim. 30 (6), 621-625

11 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POLR2J antibody

Western blot analysis of extracts of various cell lines, using POLR2J antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

POLR2J antibody

Western blot analysis of POLR2J1 in extracts from RAW264.7 cells using POLR2J antibody (Cat.-No. AP21166PU-N). Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €350.00
  Acris Antibodies GmbH

POLR2J (C-term) antibody

Formalin fixed, paraffin embedded human skeletal muscle stained with POLR2J Antibody (C-Term) Cat.-No AP53387PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

POLR2J antibody

Western Blot analysis of POLR2J expression in transfected 293T cell line by POLR2J monoclonal antibody (M02), clone 1A10.

Lane 1: POLR2J transfected lysate(13.3 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 1A10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

POLR2J antibody

Western blot analysis of extracts from RAW264.7 cells, using RPB11 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

POLR2J antibody

Western blot analysis of extracts from RAW264.7 cells, using RPB11 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

POLR2J

POLR2J Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

POLR2J (1-117, His-tag)

POLR2J Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

POLR2J (1-117, His-tag)

POLR2J Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

POLR2J

POLR2J Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

POLR2J overexpression lysate

POLR2J overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

11 Item(s)

per page
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