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POLR3A antibody

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Principal name

POLR3A antibody

Alternative names for POLR3A antibody

RNA polymerase III subunit C1, RPC1, RPC155, DNA-directed RNA polymerase III subunit RPC1, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase III subunit A, RNA polymerase III 155 kDa subunit, RNA polymerase III subunit C160

SwissProt ID

A4IF62 (Bovin), O14802 (Human), O94666 (Schpo), P04051 (Yeast), Q5ZL98 (Chick)

Gene ID

11128 (POLR3A)

Ncbi ID

9606, NP_008986, NP_008986.2, NM_007055

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of POLR3A antibody

RNA polymerase III (Pol III) is a multi-subunit complex responsible for catalyzing the transcription of DNA into RNA. POLR3A (polymerase (RNA) III (DNA directed) polypeptide
A), also known as RPC1 or RPC155, is a 1,390 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase β chain family. Existing as the largest and catalytic core component of Pol III, POLR3A functions as a DNA-dependent RNA polymerase that catalyzes the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. The gene encoding POLR3A maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolmans syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria.

ARP48725_P050 - POLR3A
Human Muscle; WB Suggested Anti-POLR3A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; POLR3A antibody - middle region (ARP48725_P050) in Human Muscle cells using Western Blot
AP21068PU-N - POLR3A
Immunohistochemistry (IHC) analyzes of POLR3A antibody (Cat.-No.: AP21068PU-N) in paraffin-embedded human breast carcinoma tissue.
AP21068PU-N - POLR3A
Western blot (WB) analysis of POLR3A antibody (Cat.-No.: AP21068PU-N) in extracts from HUVEC cells.
C15473-1 - POLR3A
Western blot analysis of extracts from COS/HuvEc cells, using RPC1 Antibody. The lane on the right is treated with the synthesized peptide.
TA308085 - POLR3A
Sample (30 ug of whole cell lysate). A: HCT116. 5% SDS PAGE. TA308085 diluted at 1:500.
TA308085 - POLR3A
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using POLR3A(TA308085) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
TA315152 - POLR3A
Western blot analysis of extracts from HUVEC cells and COS cells, using RPC1 antibody.The lane on the right is treated with the synthesized peptide.

8 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POLR3A antibody
  Rabbit polyclonal antibody to POLR3A (polymerase (RNA) III (DNA directed) polypeptide A, 155kDa)  
Sample (30 ug of whole cell lysate). A: HCT116. 5% SDS PAGE. TA308085 diluted at 1:500. Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

POLR3A antibody
  Rabbit polyclonal anti-RPC1 / POLR3A antibody  
Western blot analysis of extracts from HUVEC cells and COS cells, using RPC1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POLR3A antibody
Immunohistochemistry (IHC) analyzes of POLR3A antibody (Cat.-No.: AP21068PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
+1 additional image

POLR3A antibody
Western blot analysis of extracts from COS/HuvEc cells, using RPC1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €230.00
  Assay Biotech

POLR3A antibody
Western blot analysis of extracts from COS/HuvEc cells, using RPC1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

POLR3A - human gene knockout kit via CRISPR
POLR3A - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

POLR3A - human gene knockout kit via CRISPR
POLR3A - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, POLR3A (mGFP-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide A, 155kDa (POLR3A), 200 uL, >10^7 TU/mL
Lenti ORF particles, POLR3A (mGFP-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide A, 155kDa (POLR3A), 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.
 

8 Item(s)

per page

Available Filters

Product Type
  1. CRISPR (2)
  2. Human Lenti ORF Particle (1)
  3. Primary Antibody (5)
Clonality
  1. Polyclonal (5)
Target
  1. POLR3A (5)
Reactivity
  1. Human (Hu) (5)
  2. Mouse (Ms) (3)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Enzyme Immunoassay (E) (3)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  4. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (4)
  2. Purified (1)
Host
  1. Rabbit (5)
Images
  1. 1 image (5)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Immunofluorescence (IF) (1)
  3. Paraffin Sections (P) (1)
Species (CRISPR)
  1. Human (2)
Donor Vector
  1. GFP-Puro (1)
  2. mBFP-Neo (1)
Manufacturer
  1. Acris - OriGene (6)
  2. Assay Biotech (2)
Service
  • OriGene EU
  • Acris Antibodies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info@acris-online.de
  • orders: ordering@acris-online.de
 
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