POLR3D antibody

Principal name

POLR3D antibody

Alternative names for POLR3D antibody

RNA polymerase III subunit C4, RPC4, BN51, BN51T, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, RNA polymerase III 47 kDa subunit, RPC53 homolog

SwissProt ID

O74857 (Schpo), P05423 (Human), P25441 (Yeast), Q5E9Z7 (Bovin), Q91WD1 (Mouse)

Gene ID

661 (POLR3D)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP)

Background of POLR3D antibody

Four independent genes encoding various variants of the hRPB11 subunit of Homo sapiens RNA polymerase II were revealed in human chromosome 7. Three genes (POLR2 J1, POLR2 J2, and POLR2 J3) form a cluster of total length of 214 530 bp in the genetic locus 7q22.1 on the long arm of chromosome 7 (contig NT_007933). The fourth gene (POLR2 J4, 31 040 bp) was localized in the cytogenetic locus 7p13 of the short arm of chromosome 7 (contig NT_007819). An analysis enabled us to refine dissimilar experimental data on the mapping of the hRPB11 subunit gene on chromosome 7. In particular, the presence of three sites of its localization according to data on hybridization with fluorescent-labeled probes (the FISH method) was explained. It was established that, upon the expression of the four human POLR2 J genes, at least 14 types of mature mRNAs encoding somewhat differing hRPB11 isoforms can be synthesized. Eleven of these mRNAs were revealed (as full-length copies or clearly identifiable fragments) in the available databases of expressed sequence tags and cDNAs. The most probable scheme of origination of the multiple genes of the POLR2 J family as a result of three consecutive segmented duplications increasing in size was proposed and substantiated. On the basis of the scheme, some assumptions on the pathways of evolution of separate human genes and the mechanisms of generation of protein diversity in higher eukaryotes were made

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POLR3D antibody

Western blot analysis of extracts from MCF-7 cells, using RPC4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POLR3D antibody

Western blot (WB) analysis of POLR3D antibody (Cat.-No.: AP21167PU-N) in extracts from MCF-7 cells. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €350.00
  Acris Antibodies GmbH

POLR3D antibody

Western Blot analysis of POLR3D expression in transfected 293T cell line (H00000661-T03) by POLR3D MaxPab polyclonal antibody.

Lane 1: POLR3D transfected lysate(43.78 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

POLR3D antibody

Detection limit for recombinant GST tagged POLR3D is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 4E6 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

POLR3D antibody

Western blot analysis of BMX polyclonal antibody in rat kidney tissue lysate. Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

POLR3D antibody

Western blot analysis of extracts from MCF-7 cells, using RPC4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

POLR3D antibody

Western blot analysis of extracts from MCF-7 cells, using RPC4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

POLR3D antibody

POLR3D Antibody [NBP1-28737] - Detection of Human POLR3D by Western Blot and Immunoprecipitation.  Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.   Antibodies: Affinity purified rabbit anti-POLR3D antibody NBP1-28737 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate.  POLR3D was also immunoprecipitated by rabbit anti-POLR3D antibody NBP1-28726, which recognizes a downstream epitope.  For blotting immunoprecipitated POLR3D, the ReliaBLOT®  Reagents and Procedures were used.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B). Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

POLR3D antibody

POLR3D Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

POLR3D antibody

POLR3D Rabbit IgG Aff - Purified Hu, Ms E, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

POLR3D

POLR3D Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

POLR3D

POLR3D Human
2 µg / €300.00
  Abnova Taiwan Corp.

POLR3D

POLR3D Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

POLR3D

POLR3D Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

POLR3D 293T Cell Transient Overexpression Lysate(Denatured)

POLR3D 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

POLR3D overexpression lysate

POLR3D overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

16 Item(s)

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