Polycomb protein EED antibody

Principal name

Polycomb protein EED antibody

Alternative names for Polycomb protein EED antibody

hEED, EED, WAIT-1, WAIT1, WD protein associating with integrin cytoplasmic tails 1, embryonic ectoderm development

SwissProt ID

O75530 (Human), Q3SZ25 (Bovin), Q5ZKH3 (Chick), Q921E6 (Mouse)

Gene ID

8726 (EED), 13626 (Eed)

Available reactivities

Bov (Bovine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of Polycomb protein EED antibody

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function.This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene.

General readings

1. Rietzler M, Bittner M, Kolanus W, et al. The human WD repeat protein WAIT-1 specifically interacts with the cytoplasmic tails of beta7-integrins. J. Biol. Chem. 1998; 273:27459-66.
2. Brock HW and Fisher CL. Maintenance of gene expression patterns. Dev Dyn. 2005; 232:633-55.
3. Levine SS, King IF, Kingston RE, et al. Division of labor in polycomb group repression. Trends Biochem. Sci. 2004; 29: 478-85.
4. Van der Vlag J and Otte AP. Transcriptional repression mediated by the human Polycomb-group protein EED involves histone deacetylation. Nat. Genet.1999; 23:474-8.
5. Rakotobe D, Tardy JC, André P, et al. Human Polycomb group EED protein negatively affects HIV-1 assembly and release. Retrovirology 2007; 4:37.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Polycomb protein EED antibody

  Rabbit Polyclonal Anti-EED Antibody  
Host: Rabbit; Target Name: EED; Sample Tissue: Human Fetal Stomach; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze ICC/IF, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Polycomb protein EED antibody

  Rabbit polyclonal antibody to EED (embryonic ectoderm development)  
EED antibody detects EED protein by Western blot analysis. A. 30 ug H1299 whole cell lysate/extract. B. 30 ug HCT116 whole cell lysate/extract. 10 % SDS-PAGE. EED antibody (TA308744) dilution: 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Polycomb protein EED (N-term) antibody

  Rabbit Polyclonal EED Antibody  
Western blot analysis of EED in human heart tissue lysate with Eed antibody at 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Polycomb protein EED antibody

  Rabbit anti-EED Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using EED antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Polycomb protein EED antibody

  Rabbit Polyclonal Anti-EED Antibody  
Host: Rabbit; Target Name: EED; Sample Tissue: HepG2 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

Eed - mouse gene knockout kit via CRISPR

Eed - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Eed - mouse gene knockout kit via CRISPR

Eed - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Eed - mouse gene knockout kit via CRISPR

Eed - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Eed - mouse gene knockout kit via CRISPR

Eed - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Polycomb protein EED (transcript variant 1)

  Recombinant protein of human embryonic ectoderm development (EED), transcript variant 1  
Polycomb protein EED Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Polycomb protein EED (full length, N-term HIS tag, transcript variant 1)

  Purified recombinant protein of Human embryonic ectoderm development (EED), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
Polycomb protein EED Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Polycomb protein EED (1-441, His-tag)

Polycomb protein EED Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Polycomb protein EED (1-441, His-tag)

Polycomb protein EED Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

EED overexpression lysate

EED overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Eed (GFP-tagged) - Mouse embryonic ectoderm development (Eed), 200 uL, >10^7 TU/mL

Lenti ORF particles, Eed (GFP-tagged) - Mouse embryonic ectoderm development (Eed), 200 uL, >10^7 TU/mL  
200 µl / €1,040.00
  OriGene Technologies, Inc.

Lenti ORF particles, Eed (Myc-DDK-tagged) - Mouse embryonic ectoderm development (Eed), 200 uL, >10^7 TU/mL

Lenti ORF particles, Eed (Myc-DDK-tagged) - Mouse embryonic ectoderm development (Eed), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.

16 Item(s)

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