POU2AF1 / BOB1 antibody

Principal name

POU2AF1 / BOB1 antibody

Alternative names for POU2AF1 / BOB1 antibody

OBF1, OBF-1, OCA-B, OCAB, POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, OCT-binding factor 1

SwissProt ID

Q16633 (Human), Q2KJA4 (Bovin), Q64693 (Mouse)

Gene ID

5450 (POU2AF1), 18985 (Pou2af1)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat), Bov (Bovine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F)

Background of POU2AF1 / BOB1 antibody

POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2. A novel protein designated Bob 1 (B cell Oct binding protein 1), alternatively called OBF-1, specifically interacts with Oct-1 and Oct-2, enhancing their transcriptional efficacy. Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2. Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and/or Oct-2. The gene which encodes Bob 1 maps to human chromosome 11q23.1.

General readings

Strubin M, Newell JW, Matthias P.
OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins.
Cell. 1995 Feb 10;80(3):497-506.
PMID: 7859290

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POU2AF1 / BOB1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-POU2AF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807917)(1:150) Dilut Mouse IgG2b OTI2D4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 (C-term) antibody

AM12071PU Bob-1 antibody staining of Human Tonsil. Rabbit IgG SP92 Purified Hu P, WB
0.5 ml / €510.00
  Acris Antibodies GmbH

POU2AF1 / BOB1 (C-term) antibody

AM12071PU Bob-1 antibody staining of Human Tonsil. Rabbit IgG SP92 Purified Hu P, WB
1 ml / €730.00
  Acris Antibodies GmbH

POU2AF1 / BOB1 (C-term) antibody

AM12071PU Bob-1 antibody staining of Human Tonsil. Rabbit IgG SP92 Purified Hu P, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

POU2AF1 / BOB1 antibody

Human tonsil stained with Anti-Bob-1 antibody (Cat# AP15373PU). Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

POU2AF1 / BOB1 antibody

Human tonsil stained with Anti-Bob-1 antibody (Cat# AP15373PU). Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

POU2AF1 / BOB1 antibody

Human tonsil stained with Anti-Bob-1 antibody (Cat# AP15373PU). Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

POU2AF1 / BOB1 (C-term) antibody

Paraffin Embedded Human Spleen using POU2AF1 / BOB1 Antibody Cat.-No AP32080PU-N shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Goat Aff - Purified Bov, Can, Hu E, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

POU2AF1 / BOB1 antibody

HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human POU2AF1 with DYKDDDDK tag probed with POU2AF1 Antibody Cat.-No AP32714PU-N (0.5 µg/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with AP32714PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

POU2AF1 / BOB1 (C-term) antibody

TA302834 staining (2µg/ml) of mouse spleen lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

POU2AF1 / BOB1 antibody

Predicted cell location: Nucleus. Positive control: Human breast and thyroid cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using POU2AF1 antibody at dilution 1/15, on the right is treated with the synthetic peptide.(Originalmagnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

POU2AF1 / BOB1 antibody

POU2AF1 Antibody (Cat. #TA328146) western blot analysis in Daudi cell line lysates (35ug/lane).This demonstrates the POU2AF1 antibody detected the POU2AF1 protein (arrow). Mouse IgG1 1170CT1.1.1 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

POU2AF1 / BOB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

POU2AF1 / BOB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.(1:2000) Mouse IgG1 OTI1E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807923)(1:150) Mouse IgG1 OTI3B9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807926)(1:150) Mouse IgG1 OTI4G7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.(1:2000) Mouse IgG1 OTI2E1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807930)(1:150) Mouse IgG1 OTI2D3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POU2AF1 mouse monoclonal antibody at 1:150.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807990)(1:150) Mouse IgG1 OTI3A5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807999)(1:150) Mouse IgG1 OTI13G1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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