POU2AF1 / BOB1 antibody

Principal name

POU2AF1 / BOB1 antibody

Alternative names for POU2AF1 / BOB1 antibody

OBF1, OBF-1, OCA-B, OCAB, POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, OCT-binding factor 1

SwissProt ID

Q16633 (Human), Q2KJA4 (Bovin), Q64693 (Mouse)

Gene ID

5450 (POU2AF1), 18985 (Pou2af1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)

Background of POU2AF1 / BOB1 antibody

POU2AF1(POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al. (2002) reported that OCAB is essential for V(D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by directly enhancing Ig-kappa gene transcription in vivo. Siegel et al. (2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.

General readings

Strubin M, Newell JW, Matthias P.
OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins.
Cell. 1995 Feb 10;80(3):497-506.
PMID: 7859290

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POU2AF1 / BOB1 antibody

  POU2AF1 mouse monoclonal antibody, clone OTI2D4 (formerly 2D4)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-POU2AF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807917)(1:150) Dilut Mouse IgG2b OTI2D4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 (C-term) antibody

Paraffin Embedded Human Spleen using POU2AF1 / BOB1 Antibody Cat.-No AP32080PU-N shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Goat Aff - Purified Bov, Can, Hu E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

POU2AF1 / BOB1 antibody

HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human POU2AF1 with DYKDDDDK tag probed with POU2AF1 Antibody Cat.-No AP32714PU-N (0.5 µg/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with AP32714PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

POU2AF1 / BOB1 (C-term) antibody

  Goat Polyclonal Antibody against POU2AF1  
TA302834 staining (2µg/ml) of mouse spleen lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

POU2AF1 / BOB1 antibody

  Anti-POU2AF1 Rabbit Polyclonal Antibody  
Predicted cell location: Nucleus. Positive control: Human breast and thyroid cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using POU2AF1 antibody at dilution 1/15, on the right is treated with the synthetic peptide.(Originalmagnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

POU2AF1 / BOB1 antibody

  Mouse monoclonal anti-POU2AF1 antibody  
POU2AF1 Antibody (Cat. #TA328146) western blot analysis in Daudi cell line lysates (35ug/lane).This demonstrates the POU2AF1 antibody detected the POU2AF1 protein (arrow). Mouse IgG1 1170CT1.1.1 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

POU2AF1 / BOB1 antibody

  Rabbit Polyclonal anti-BOB-1 Antibody  
POU2AF1 / BOB1 Rabbit IgG Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POU2AF1 / BOB1 antibody

  Rabbit Monoclonal anti-BOB-1 Antibody  
POU2AF1 / BOB1 Rabbit IgG SP92 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POU2AF1 / BOB1 antibody

  Rabbit Polyclonal BOB1 Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

POU2AF1 / BOB1 antibody

  POU2AF1 mouse monoclonal antibody, clone OTI1E11 (formerly 1E11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.(1:2000) Mouse IgG1 OTI1E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 antibody

  POU2AF1 mouse monoclonal antibody, clone OTI3B9 (formerly 3B9)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807923)(1:150) Mouse IgG1 OTI3B9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 antibody

  POU2AF1 mouse monoclonal antibody, clone OTI4G7 (formerly 4G7)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807926)(1:150) Mouse IgG1 OTI4G7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 antibody

  POU2AF1 mouse monoclonal antibody, clone OTI2E1 (formerly 2E1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.(1:2000) Mouse IgG1 OTI2E1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 antibody

  POU2AF1 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807930)(1:150) Mouse IgG1 OTI2D3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 antibody

  POU2AF1 mouse monoclonal antibody, clone OTI3A5 (formerly 3A5)  
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POU2AF1 mouse monoclonal antibody at 1:150.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807990)(1:150) Mouse IgG1 OTI3A5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 antibody

  POU2AF1 mouse monoclonal antibody, clone OTI13G1 (formerly 13G1)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807999)(1:150) Mouse IgG1 OTI13G1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

POU2AF1 / BOB1 (N-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POU2AF1 Antibody (N-term) cat.no. AP53397PU-N for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

POU2AF1 / BOB1 antibody

POU2AF1 / BOB1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

POU2AF1 / BOB1 antibody

POU2AF1 polyclonal antibody ( Cat # PAB6265 ) staining ( 2 µg/ml ) of mouse spleen lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

POU2AF1 / BOB1 antibody

Immunohistochemical staining of POU2AF1 on formalin fixed, paraffin embedded human tonsil with POU2AF1 polyclonal antibody. Rabbit Hu P
0.1 ml / €390.00
  Abnova Taiwan Corp.

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