POU2F1 / OCT1 antibody

Principal name

POU2F1 / OCT1 antibody

Alternative names for POU2F1 / OCT1 antibody

OTF1, NF-A1, Octamer-binding protein 1, Oct-1, Otf-1, POU domain class 2 transcription factor 1, Octamer-binding transcription factor 1

SwissProt ID

P14859 (Human), P15143 (Chick), P16143 (Xenla), P25425 (Mouse), P31503 (Rat), Q29076 (Pig), Q9WTZ4 (Mouse)

Gene ID

5451 (POU2F1), 18986 (Pou2f1)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Can (Canine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Dot blot (Dot), Electrophoretic Mobility Shift Assay (EMSA)

Background of POU2F1 / OCT1 antibody

Organic cation transporters (OCT) are expressed in the plasma membrane of epithelial cells from a wide range of tissues, where they function in the elimination of endogenous amines, cationic drugs and other xenobiotics. The structure of OCTs consists of a 12-transmembrane-domain structure and a large extracellular hydrophilic loop. In humans, OCT1 is primarily expressed in the liver while OCT2 is expressed in the kidney. OCT3 is expressed in the placenta, skeletal muscle, prostate, aorta and liver. OCT3, also known as extraneuronal monoamine transporter, is widely expressed in different regions of the brain including the hippocampus, cerebellum and cerebral cortex. OCT3 mediates the uptake of several neuroactive agents, including dopamine, and may play an important role in the disposition of neurotransmitters and cationic neurotoxins in the brain. The genes encoding human OCT1–3 map to a conserved cluster at chromosome 6q26-q27.

General readings

1. Imai, S., et al., Mol. Biol. Cell 8, 2407-2419 (1997)
2. Ford, E., and Hernandez, N., J. Biol. Chem. 272, 16048-16055 (1997)
3. Giffin, W., et al., J. Biol. Chem. 272, 5647-5658 (1997)
4. Wilson, A. C., et al., Genes & Dev. 9, 2445-2458 (1995)
5. Lai, J., and Herr, W., PNAS. 89, 6958-6962 (1992)
Clone YL15 is used in these references.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POU2F1 / OCT1 (C-term) antibody

Human tonsil stained with anti-Oct-1 antibody Rabbit Purified Hu, Ms, Rt P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

POU2F1 / OCT1 (C-term) antibody

Human tonsil stained with anti-Oct-1 antibody Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €440.00
  Acris Antibodies GmbH

POU2F1 / OCT1 (C-term) antibody

Human tonsil stained with anti-Oct-1 antibody Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

POU2F1 / OCT1 antibody

(0.5ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

POU2F1 / OCT1 antibody

Western blot analysis of extracts from HeLa cells, using OCT-1 antibody (#TA314361).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POU2F1 / OCT1 antibody

Western blot analysis of Oct-1 in Jurkat cell lysate. Rabbit Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

POU2F1 / OCT1 antibody

Predicted band size: 76 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 50 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POU2F1 / OCT1 antibody

Predicted band size: 76 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 15 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

POU2F1 / OCT1 antibody

WB Suggested Anti-POU2F1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Transfected 293TPOU2F1 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

POU2F1 / OCT1 antibody

WB Suggested Anti-POU2F1 Antibody Titration: 1.25ug/ml; ELISA Titer: 1:62500; Positive Control: SP2/0 cell lysate Rabbit IgG Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

POU2F1 / OCT1 antibody

Western blot analysis of extracts of various cell lines, using POU2F1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

POU2F1 / OCT1 antibody

WB Suggested Anti-POU2F1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

POU2F1 / OCT1 antibody

Western blot analysis on HeLa cell lysate using OCT1 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

POU2F1 / OCT1 antibody

Western blot analysis of Oct-1 expression in Jurkat (Lane 1) and MCF-7 (Lane 2) using AM20290AF-N Oct1 antibody. Mouse IgG1 YL15 Azide Free Hu, Ms EMSA, IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

POU2F1 / OCT1 control peptide

POU2F1 / OCT1 Purified
50 µg / €190.00
  Acris Antibodies GmbH

POU2F1 / OCT1 antibody

Immunohistochemistry (IHC) analyzes of OCT1/OTF1 antibody (Cat.-No.: AP06254PU-N) in paraffin-embedded human lymph node tissue.
	.
	
	. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

POU2F1 / OCT1 antibody

Western blot analysis of Oct-1 antibody (Cat.-No.: AP20339PU-N) in extracts from COLO205 cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

POU2F1 / OCT1 (Center) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue using POU2F1 antibody (Center)  cat.no. AP53398PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

POU2F1 / OCT1 antibody

Immunofluorescence of purified MaxPab antibody to POU2F1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

POU2F1 / OCT1 antibody

POU2F1 / OCT1 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

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