POU3F2 antibody

Principal name

POU3F2 antibody

Alternative names for POU3F2 antibody

BRN2, Brain-2, Brn-2, OCT7, Oct-7, OTF7, OTF-7, POU domain class 3 transcription factor 2, Brain-specific homeobox/POU domain protein 2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Octamer-binding transcription factor 7

SwissProt ID

P20265 (Human), P31360 (Mouse), P56222 (Rat)

Gene ID

5454 (POU3F2)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), African clawed frog, Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of POU3F2 antibody

N-Oct-3 (POU3F2) is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1) and Oct2 (POU2F2), and the pituitary protein Pit1 (PIT1). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. N-Oct-3 is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176), and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression.[supplied by OMIM].

General readings

Hara Y, Rovescalli AC, Kim Y, et al. Structure and evolution of four POU domain genes expressed in mouse brain. Proc. Natl. Acad. Sci. USA 1992; 89:3280-4.
McEvilly RJ, de Diaz MO, Schonemann MD, et al. Transcriptional regulation of cortical neuron migration by POU domain factors. Science 2002; 295:1528-32.
Huang YT, Iwamoto K, Kurosaki T, et al. The neuronal POU transcription factor Brn-2 interacts with Jab1, a gene involved in the onset of neurodegenerative diseases. Neurosci. Lett. 2005; 382:175-8
Goodall J, Carreira S, Denat L, et al. Brn-2 represses microphthalmia-associated transcription factor expression and marks a distinct subpopulation of microphthalmia-associated transcription factor-negative melanoma cells. Cancer Res. 2008; 68:7788-94

18 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

POU3F2 antibody

(0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

POU3F2 antibody

Western blot analysis of POU3F2 in 3T3 cell lysate with POU3F2 antibody at (A) 1 and (B) 2 µg/mL. Rabbit Purified Hu, Ms, Rt E, WB
0.1 mg / €345.00
  OriGene Technologies, Inc.

POU3F2 (C-term) antibody

Western blot analysis of POU3F2 in 3T3 cell lysate with POU3F2 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

POU3F2 antibody

WB Suggested Anti-POU3F2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Transfected 293T Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

POU3F2 (Center) antibody

Western blot analysis in K562 cell line lysates (35ug/lane) detecting POU3F2 protein by the use of POU3F2 antibody cat. no. AP53399PU-N Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

POU3F2 (C-term) Control Peptide

POU3F2
50 µg / €160.00
  Acris Antibodies GmbH

POU3F2 antibody

POU3F2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

POU3F2 antibody

POU3F2 monoclonal antibody (M01), clone 6F6 Western Blot analysis of POU3F2 expression in HeLa NE ( Cat # L013V3 ). Mouse IgG2b 6F6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

POU3F2 antibody

Detection limit for recombinant GST tagged POU3F2 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2b 1H5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

POU3F2 antibody

Mouse adult cortex; . Sample Type :. Mouse adult cortex. Primary Antibody Dilution :. 1:1000. Secondary Antibody :. Donkey anti rabbit IgG Alexa 594. Secondary Antibody Dilution :. 1:1000. Gene Name :. POU3F2 . Submitted by :. Dr. Jin Hee Lee, Ph.D., Winthrop-University Hospital; POU3F2 antibody - middle region (ARP31218_P050) in Mouse adult cortex cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

POU3F2 antibody

Human 293T; WB Suggested Anti-POU3F2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; POU3F2 antibody - C-terminal region (P100858_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

POU3F2 antibody

POU3F2 Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

POU3F2

POU3F2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

POU3F2

POU3F2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

POU3F2

POU3F2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

POU3F2

POU3F2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

POU3F2

POU3F2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

POU3F2 overexpression lysate

POU3F2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

18 Item(s)

per page
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