PPAR-alpha antibody

Principal name

PPAR-alpha antibody

Alternative names for PPAR-alpha antibody

NR1C1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha, Nuclear receptor subfamily 1 group C member 1

SwissProt ID

P23204 (Mouse), P37230 (Rat), Q07869 (Human)

Gene ID

5465 (PPARA), 19013 (Ppara)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Rb (Rabbit), Bov (Bovine), Hst (Hamster), Chk (Chicken), African clawed frog, Mky (Monkey), Sh (Sheep), Chimp (Chimpanzee), Eq (Equine), Gt (Goat)

Available hosts

Mouse, Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Electrophoretic Mobility Shift Assay (EMSA), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of PPAR-alpha antibody

Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing hiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPARgamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

General readings

Roberts RA et al. Evidence for the suppression of apoptosis by the peroxisome proliferator activated receptor alpha (PPAR alpha). Carcinogenesis 19:43-8 (1998). PubMed PMID: 9472691
Lavinsky RM et al. Diverse signaling pathways modulate nuclear receptor recruitment of N-CoR and SMRT complexes. Proc Natl Acad Sci U S A 95:2920-5 (1998). PubMed PMID: 9501191
Kilgore MW et al. MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response. Mol Cell Endocrinol 129:229-35 (1997). PubMed PMID: 9202406

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPAR-alpha antibody

Anti-PPARA mouse monoclonal antibody (TA503540) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA(RC216176). Mouse IgG1 OTI1E8 Purified Hu, Ms ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PPAR-alpha antibody

Anti-PPARA mouse monoclonal antibody (TA503543) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA(RC216176). Mouse IgG1 OTI2E7 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAR-alpha antibody

Western blot analysis of mouse brain lysate (Lane 1: 70ug; Lane 2: 80ug; Lane 3: 90ug lysate) with anti-PPAR alpha antibody diluated at 1:2000. (Predicted size: 52kDa; observed size: ~46kDa) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPAR-alpha antibody

Western blot analysis of extracts from NIH/3T3 cells, using PPAR a (Ab-21) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPAR-alpha (N-term) antibody

Anti-PPAR alpha (N -terminal specific) (Rabbit) to detect a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 µg of lysate was loaded per lane for SDS-PAGE.  Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization. Rabbit Aff - Purified Bov, Can, Hst, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPAR-alpha antibody

Predicted band size: 52 kDa . Positive control: 231 and Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: 231 cell lysate Lane2: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPAR-alpha antibody

Predicted band size: 52 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPAR-alpha antibody

WB Suggested Anti-PPARA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: OVCAR-3 cell lysate Rabbit IgG Purified Bov, Eq, Gt, Hu, Ms, Por, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPAR-alpha antibody

Anti-PPARA mouse monoclonal antibody (TA503529) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA(RC216176). Mouse IgG1 OTI2D10 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAR-alpha antibody

Anti-PPARA mouse monoclonal antibody (TA503539) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA(RC216176). Mouse IgG1 OTI3G3 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAR-alpha antibody

Anti-PPARA mouse monoclonal antibody (TA503541) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA(RC216176). Mouse IgG1 OTI1E11 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAR-alpha antibody

Anti-PPARA mouse monoclonal antibody (TA503542) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA(RC216176). Mouse IgG1 OTI2F3 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAR-alpha antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PPARA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503545) Mouse IgG1 OTI2G6 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAR-alpha antibody

Western blot analysis of PPAR-alpha expression in lysate from Jurkat cells (Lane 1, 2), Rat Liver (Lane 3) and rat kidney (Lane 4) Rabbit Aff - Purified Can, Hu, Ms, Por, Rt WB
0.1 mg / €410.00
  Acris Antibodies GmbH

PPAR-alpha (N-term) antibody

PPAR-alpha Rabbit IgG Purified Hu E, WB
50 µg / €320.00
  Acris Antibodies GmbH

PPAR-alpha (1-18) antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Liver. Rabbit Aff - Purified Bov, Can, Hst, Hu, Mky, Ms, Por, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PPAR-alpha (22-36) antibody

AP08238PU-N PPAR-alpha antibody staining of Formalin-Fixed Paraffin-Embedded Human Kidney at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Por, Prim, Rb, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PPAR-alpha antibody

Western blot analysis of PPAR-α antibody (AP20267PU-N) in extracts from NIH/3T3 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PPAR-alpha (Center) antibody

Western blot analysis of PPAR-alpha Antibody (Center) Cat.-No AP53407PU-N in mouse liver tissue lysates (35ug/lane). This demonstrates the PPARA antibody detected the PPARA protein (arrow). Rabbit Ig Aff - Purified Hu, Ms F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PPAR-alpha antibody

Immunofluorescence of purified MaxPab antibody to PPARA on HepG2 cell. [antibody concentration 20 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

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