PPAR-delta antibody

Principal name

PPAR-delta antibody

Alternative names for PPAR-delta antibody

PPAR-beta, Nuclear hormone receptor 1, NUCI, PPARD, NR1C2, PPARB, Peroxisome proliferator-activated receptor delta, Nuclear receptor subfamily 1 group C member 2

SwissProt ID

P35396 (Mouse), Q03181 (Human)

Gene ID

19015 (Ppard), 5467 (PPARD)

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Rb (Rabbit), Ze (Zebrafish), Mky (Monkey), Sh (Sheep), Chimp (Chimpanzee), Dros (Drosophila), Eq (Equine), GP (Guinea Pig), Ye (Yeast)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), Electrophoretic Mobility Shift Assay (EMSA)

Background of PPAR-delta antibody

Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253 (2010).
Christopoulos, P., et al. Ann. N. Y. Acad. Sci. 1205, 185-191 (2010).
Dunn, S.E., et al. J. Exp. Med. 207(8):1599-1608(2010).
Eynon, N., et al. Mitochondrion (2010) In press.
Jguirim-Souissi, I., et al. Genet. Mol. Res. 9(3):1326-1333 (2010).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPAR-delta antibody

Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-PPARD rabbit polyclonal antibody. (TA590579) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPAR-delta (430-441) antibody

Immunohistochemistry Image: Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, GP, Hst, Hu, L, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PPAR-delta (Isoform 1) antibody

TA302579 (0.3µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPAR-delta (N-term) antibody

Anti-PPAR delta to detect a predominant band at ~ 43 kDa corresponding to PPAR delta present in mouse heart whole cell lysates.  Approximately 30 µg of lysate was loaded per lane for SDS-PAGE.  Detection occurred using a 1:750 dilution of primary antibody overnight at 4° C followed reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L)  for 45 min at RT.  Rabbit Aff - Purified Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPAR-delta antibody

Predicted band size: 50 kDa. Positive control: Mouse heart tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPAR-delta antibody

PPARD Antibody (C-term) (Cat. #TA324526) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the PPARD antibody detected the PPARD protein (arrow). Rabbit IgG Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

PPAR-delta antibody

WB Suggested Anti-PPARD Antibody Titration: 1.25ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPAR-delta antibody

Western blot analysis of extracts of various cell lines, using PPARD antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPAR-delta antibody

WB Suggested Anti-PPARD Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: 721_B cell lysate; PPARD is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPAR-delta antibody

WB Suggested Anti-PPARD Antibody Titration: 0.625 ug/ml; ELISA Titer: 1: 1562500; Positive Control: LN18 cell lysate; PPARD is strongly supported by BioGPS gene expression data to be expressed in Human LN18 cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPAR-delta (N-term) antibody

PPAR-delta Rabbit IgG Purified Hu, Mky, Ms, Rb, Rt E, WB
50 µg / €310.00
  Acris Antibodies GmbH

PPAR-delta (1-14) antibody

AP07663PU-N PPAR Delta antibody staining of  Formalin-Fixed Paraffin-Embedded Human Liver at 2.5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Chimp, Hu, Ms, Rb E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PPAR-delta (39-54) antibody

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hu, Mky, Op, Prim, Rb, Tr ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PPAR-delta (C-term) antibody

Western blot analysis of PPAR-delta Antibody (C-term) Cat.-No AP53408PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the PPARD antibody detected the PPARD protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PPAR-delta antibody

PPAR-delta Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PPAR-delta antibody

PPAR-delta Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PPAR-delta antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between HDAC2 and PPARD HeLa cells were stained with anti-HDAC2 rabbit purified polyclonal 1:1200 and anti-PPARD mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 4E3-1B11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PPAR-delta antibody

PPARD monoclonal antibody (M03), clone 1G4 Western Blot analysis of PPARD expression in K-562 ( Cat # L009V1 ). Mouse IgG 1G4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PPAR-delta antibody

PPARD polyclonal antibody ( Cat # PAB6679 ) ( 0.3 µg/ml ) staining of Jurkat lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PPAR-delta antibody

Immunoblotting of Ppard polyclonal antibody ( Cat # PAB11322 ) is shown to detect a predominant band at ~ 43 kDa corresponding to Ppard present in mouse heart tissue lysates.
Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band ( data not shown ).
Approximately 30 µg of lysate was loaded per lane for SDS-PAGE.
Detection occurred using a 1 : 750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4°C followed reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature ( 800 nm channel, green ).
IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Rabbit Chimp, Hu, Ms, Rb, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

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