PPAR-gamma antibody

Principal name

PPAR-gamma antibody

Alternative names for PPAR-gamma antibody

PPARG, NR1C3, Peroxisome proliferator-activated receptor gamma

SwissProt ID

O18971 (Bovin), O19052 (Rabit), O62807 (Pig), O88275 (Rat), P37231 (Human), Q15180 (Human)

Gene ID

5468 (PPARG), 25664 (Pparg)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Por (Porcine), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Rt (Rat), African clawed frog, Hst (Hamster), Duck, GP (Guinea Pig), Mink, Sq (Squirrel), Mky (Monkey), Macaque, Gt (Goat), Sh (Sheep), Eq (Equine), Ze (Zebrafish)

Available hosts

Chicken, Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunohistochemistry (IHC), Electrophoretic Mobility Shift Assay (EMSA), Immunoprecipitation (IP), ChIP assay (ChIP), Frozen Sections (C)

Background of PPAR-gamma antibody

Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. The target genes of PPAR-alpha and PPAR-delta are primarily involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

General readings

Berger J.. et al. (2002) Annu Rev Med . 53:409-35.
Michalik L .,et al. (2006) Pharmacol Rev. 58(4):726-41.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPAR-gamma antibody

Western blot analysis of rat testis lysates with anti-PPARG antibody (1:500). Rabbit IgG Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPAR-gamma (PPARG2) (N-term) antibody

WB using Anti-PPAR2 antibody shows detection of PPAR2 protein in a mouse 3T3 whole cell lysate (lane 1 arrowhead). Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose. Primary antibody was used at a 1:2,000 dilution. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 Conjugated Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Rabbit Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPAR-gamma antibody

Predicted cell location: Cytoplasm. Positive control: Human ovarian and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using PPARG antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPAR-gamma antibody

Predicted cell location: Cytoplasm. Positive control: Human ovarian and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using PPARG antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPAR-gamma antibody

Western blot analysis of extracts of various cell lines, using PPARG antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPAR-gamma antibody

Western blot analysis of PPAR-gamma expression in HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPAR-gamma antibody

WB Suggested Anti-PPARG Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Can, Hu, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPAR-gamma antibody

WB Suggested Anti-PPARG Antibody Titration: 1.25ug/ml; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPAR-gamma antibody

Western Blot: PPAR gamma Antibody [TA336911] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate. Rabbit Purified Hu, Ms C, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPAR-gamma antibody

WB Suggested Anti-PPARG Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Can, Hu, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPAR-gamma antibody

Host: Rabbit; Target Name: Pparg; Sample Tissue: Rat Kidney lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPAR-gamma antibody

Western blot detection of PPAR gamma (C-terminus) in 3T3-L1 and Mouse Lung cell lysates using PPAR gamma (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:54KDa.Observed band size:54KD. Mouse IgG1 1G7-G4-G12 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPAR-gamma antibody

WB using the antibody against PPARG. The antibody was diluted 1:2,000 in TBS-Tween containing 3% skimmed milk. Image shows the result of 293T cells transfected with pNTAP-PPARG (lane 1) and of non-transfected cells (lane 2). The position of the protein of interest is indicated on the right the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu, Ms E
50 µg / €325.00
  OriGene Technologies, Inc.

PPAR-gamma antibody

ELISA: PPAR gamma Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPAR-gamma antibody

Western blot analysis using PPARG antibody Cat.-No AM06243SU-N against truncated PPARG-His recombinant protein (Lane 1) and full-length PPARG (aa1-477) transfected CHO-K1 cell lysate (Lane 2). Mouse IgG1 3A4A9 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

PPAR-gamma (170-270) antibody

Formalin-Fixed, Paraffin-Embedded Human Adrenal stained with PPARG antibody Cat.-No AM20438PU-N at 1/200 dilution after heat-induced antigen retrieval. Mouse IgG1 3A4A9, 1E6A1 Ascites Hu, Mky E, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

PPAR-gamma (isoform CRA-c) antibody

Western blot analysis of PPARgamma in lysate from 3T3 cells (Lane 1) and Jurkat Cells (Lane 2,3) using antibody AP00649PU-N Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €370.00
  Acris Antibodies GmbH

PPAR-gamma (Isoform 1) antibody

PPAR-gamma Rabbit IgG Purified Can, Duck, GP, Hst, Hu, Mink, Mky, Ms, Rb, Rt E
50 µg / €300.00
  Acris Antibodies GmbH

PPAR-gamma antibody

Immunohistochemistry (IHC) analyzes of PPAR-γ antibody (Cat.-No.: AP06573PU-N) in paraffin-embedded human placenta tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PPAR-gamma (PPARG2) antibody

Formalin-Fixed Paraffin-Embedded Human Prostate stained with PPARG antibody Cat.-No AP07257PU-N at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-streptavidin and Chromogen. Rabbit Aff - Purified Can, Hu, Ms, Por E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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