PPAT antibody

Principal name

PPAT antibody

Alternative names for PPAT antibody

Amidophosphoribosyltransferase, ATase, GPAT, Glutamine phosphoribosylpyrophosphate amidotransferase

SwissProt ID

P04046 (Yeast), P0AG16 (Ecoli), P28173 (Chick), P35433 (Rat), P41390 (Schpo), Q06203 (Human), Q27601 (Drome)

Gene ID

5471 (PPAT), 5065471 (A4THE9)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Rt (Rat), African clawed frog, Ms (Mouse), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F)

Background of PPAT antibody

PPAT is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. Its gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.

General readings

Bera,A.K., (1999) J. Biol. Chem. 274 (51), 36498-36504

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPAT antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504769) Mouse IgG1 OTI1B8 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PPAT antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504706) Mouse IgG1 OTI1B11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAT antibody

Hum. Adult Placenta; Host:Rabbit. Target Name:PPAT. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; PPAT antibody - C-terminal region (ARP46080_P050) in Hum. Adult Placenta cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

PPAT antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (RC201144, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT. Mouse IgG2b OTI3C6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAT antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (RC201144, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT. Mouse IgG1 OTI2C2 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAT antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (RC201144, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT. Mouse IgG1 OTI1C7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAT antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (RC201144, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT. Mouse IgG1 OTI1E1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAT antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (RC201144, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT. Mouse IgG1 OTI1C2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAT antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (RC201144, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT. Mouse IgG1 OTI1C4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAT antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (RC201144, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT. Mouse IgG1 OTI1D9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAT antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPAT monoclonal antibody. Mouse IgG1 OTI1B2 Purified Hu, Mky, Ms F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPAT (Center) antibody

Western blot analysis of anti-PPAT Antibody (Center) (Cat.#AP12386PU-N) in HepG2 cell line lysates (35ug/lane).PPAT(arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PPAT antibody

Western Blot analysis of PPAT expression in transfected 293T cell line (H00005471-T01) by PPAT MaxPab polyclonal antibody.

Lane 1: PPAT transfected lysate(56.87 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PPAT antibody

PPAT Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

PPAT antibody

PPAT Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PPAT antibody

Western blot analysis of PPAT polyclonal antibody ( Cat # PAB2598 ) in HepG2 cell line lysates ( 35 µg/lane ) .PPAT ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PPAT antibody

Human kidney; PPAT antibody - N-terminal region (ARP46079_P050) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

PPAT antibody

Western Blot: phosphoribosyl pyrophosphate amidotransferase Antibody [NBP1-52964] - This Anti-PPAT antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 0.25ug/ml. Rabbit Aff - Purified Hu P, WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PPAT

PPAT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

PPAT

PPAT Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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