PPME1 antibody

Principal name

PPME1 antibody

Alternative names for PPME1 antibody

PME1, PME-1, Protein phosphatase methylesterase 1

SwissProt ID

P38796 (Yeast), Q58DN4 (Bovin), Q8BVQ5 (Mouse), Q9BIB3 (Caeel), Q9P7D2 (Schpo), Q9Y570 (Human)

Gene ID

51400 (PPME1), 72590 (Ppme1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)

Background of PPME1 antibody

Carboxymethylation of proteins is a highly conserved means of regulation in eukaryotic cells. The serine hydrolase protein phosphatase methylesterase-1 (PME-1) regulates the methylesterification state of protein phosphatase 2A (PP2A) and has been implicated in cancer and neurodegeneration disease. PME-1 is conserved from yeast to human and contains a motif found in lipases having a catalytic triad-activated serine as their active site nucleophile. Maturation of catalytic subunit of PP2A is under the surveillance of the PP2A methylesterase, PME1, which upon malfunction of PP2A biogenesis, prevents premature generation of the active C subunit and holoenzyme assembly by counteracting the untimely methylation of the C subunit. PME-1 has been recently identified as a protector of sustained ERK pathway activity in malignant gliomas. Mechanistically, PME-1 is shown to support ERK pathway signaling upstream of Raf, but downstream of growth factor receptors and protein kinase C. In malignant gliomas, PME-1 expression levels correlate with both ERK activity and cell proliferation .

General readings

Gagnon S.N. et al. (2002) Biochem. J. 368:263-271.

  1. Quick-Filters:
  2. Highly Validated (5)

Items 1 to 20 of 26 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPME1 antibody

  anti-PPME1 mouse monoclonal antibody, clone OTI10B2 (formerly 10B2)  
Anti-PPME1 mouse monoclonal antibody (TA500769) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1(RC200009). Mouse IgG1 OTI10B2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPME1 antibody

  anti-PPME1 mouse monoclonal antibody, clone OTI4A12 (formerly 4A12)  
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500821, Dilution 1:50) Mouse IgG1 OTI4A12 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPME1 antibody

  anti-PPME1 mouse monoclonal antibody, clone OTI10A12 (formerly 10A12)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PPME1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500822, Dilution 1:50) Mouse IgG2a OTI10A12 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPME1 antibody

  anti-PPME1 mouse monoclonal antibody, clone OTI7F12 (formerly 7F12)  
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500823, Dilution 1:50) Mouse IgG1 OTI7F12 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PPME1 antibody

  anti-PPME1 mouse monoclonal antibody, clone OTI6H5 (formerly 6H5)  
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500840, Dilution 1:50) Mouse IgG1 OTI6H5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPME1 antibody

  Rabbit Polyclonal Anti-PPME1 Antibody - N-terminal region  
WB Suggested Anti-PPME1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysatePPME1 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPME1 antibody

  Rabbit Polyclonal Anti-PPME1 Antibody - N-terminal region  
Host:Rabbit; Target Name:PPME1; Sample Tissue:Human Fetal Brain; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPME1 antibody

  Mouse Monoclonal anti-PME-1 Antibody  
Hela whole cell lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-PME-1 antibody (clone 20G3SC). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse 20G3SC Purified Hu WB
0.1 mg / €280.00
  OriGene Technologies, Inc.

PPME1 antibody

  anti-PPME1 mouse monoclonal antibody, clone OTI2C5 (formerly 2C5)  
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500768, Dilution 1:50) Mouse IgG1 OTI2C5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPME1 antibody

Western Blot analysis of PPME1 expression in transfected 293T cell line (H00051400-T01) by PPME1 MaxPab polyclonal antibody.

Lane 1: PPME1 transfected lysate(42.46 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PPME1 antibody

Western Blot analysis of PPME1 expression in transfected 293T cell line (H00051400-T01) by PPME1 MaxPab polyclonal antibody.

Lane 1: PPME1 transfected lysate(42.46 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PPME1 antibody

Human Fetal Brain; Host:Rabbit. Target Name:PPME1. Sample Tissue:Human Fetal Brain. Antibody Dilution: 1.0ug/ml.; PPME1 antibody - N-terminal region (ARP56844_P050) in Human Fetal Brain cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PPME1 antibody

Human brain; Immunohistochemistry with Brain, cortex tissue at an antibody concentration of 5ug/ml using anti-PPME1 antibody (ARP56845_P050); PPME1 antibody - N-terminal region (ARP56845_P050) in Human brain cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

PPME1 - human gene knockout kit via CRISPR

PPME1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PPME1 - human gene knockout kit via CRISPR

PPME1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PPME1 - human gene knockout kit via CRISPR

PPME1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PPME1 - human gene knockout kit via CRISPR

PPME1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PPME1

  Recombinant protein of human protein phosphatase methylesterase 1 (PPME1)  
PPME1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PPME1 (1-386, His-tag)

PPME1 Human Purified > 95 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

PPME1 (1-386, His-tag)

PPME1 Human Purified > 95 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Items 1 to 20 of 26 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn