PPP1CA antibody

Principal name

PPP1CA antibody

Alternative names for PPP1CA antibody

PPP1A, PP-1A, Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

SwissProt ID

P62136 (Human), P62137 (Mouse), P62138 (Rat), P62139 (Rabit), Q3T0E7 (Bovin), Q8WMS6 (Canfa)

Gene ID

5499 (PPP1CA), 100009298

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Rb (Rabbit), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of PPP1CA antibody

PPP1CA is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory.The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

General readings

1. Barker, H. M.; Jones, T. A.; da Cruz e Silva, E. F.; Spurr, N. K.; Sheer, D.; Cohen, P. T. W.: Localization of the gene encoding a type I protein phosphatase catalytic subunit to human chromosome band 11q13. Genomics 7: 159-166, 1990.
2 Genoux, D.; Haditsch, U.; Knobloch, M.; Michalon, A.; Storm, D.; Mansuy, I. M. : Protein phosphatase 1 is a molecular constraint on learning and memory. Nature 418: 970-975, 2002.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPP1CA antibody

Western blot analysis of extracts of various cell lines, using PPP1CA antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPP1CA antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150) Mouse IgG1 OTI6E5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPP1CA antibody

Immunohistochemistry:  AM03118PU-N PPP1CA antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2a 4G3 Purified Hu E, ICC/IF, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

PPP1CA antibody

Immunohistochemistry:  AM03118PU-N PPP1CA antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2a 4G3 Purified Hu E, ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

PPP1CA antibody

Predicted band size: 38 kDa . Positive control: HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPP1CA antibody

Host: Rabbit; Target Name: Ppp1ca; Sample Tissue: Mouse Spleen lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPP1CA antibody

WB Suggested Anti-PPP1CA Antibody ; Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate; Primary Antibody Dilution : 1:1000; Secondary Antibody : Goat anti-rabbit-HRP; Secondry Antibody Dilution : 1:5000; Submitted by: Jose Luis Rosa, U Rabbit IgG Purified Bov, Hu, Ms, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPP1CA antibody

Western blot detection of PPP1A in MCF7,K562,Hela,HEPG2 and Jurkat whole cell lysates using PPP1A mouse mAb (1:1000 diluted).Predicted band size: 38KDa.Observed band size: 38KDa. Mouse IgG1 1C11-C10-H5-E9 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPP1CA antibody

Western blot detection of PPP1A in HepG2,MCF7 and Hela cell lysates using PPP1A mouse mAb (1:1000 diluted).Predicted band size:37KDa.Observed band size:37KDa. Mouse IgG1 1H1-F7-D8 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPP1CA antibody

Western blot analysis using PPP1A antibody Cat.-No AM06514SU-N against Hela (1) and NIH/3T3 (2) cell lysate. Mouse IgG1 6D1 Ascites Hu, Ms E, WB
0.1 ml / €360.00
  Acris Antibodies GmbH

PPP1CA antibody

Western blot analysis using PPP1CA mouse mAb against Hela (1), HepG2 (2), MCF-7 (3), Jurkat (4) and A549 (5) cell lysate. Mouse IgG1 5E9 Ascites Hu E, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

PPP1CA antibody

PPP1CA Mouse IgG2b PP-1A Purified Hu, Ms, Rb, Rt ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

PPP1CA antibody

PP1 alpha antibody western blot of total rat brain homogenate. (+) indicates staining done in the presence of specific blocking peptide or (-) Peptide Control. Rabbit IgG Purified Bov, Hu, Ms, Rt IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

PPP1CA antibody

PPP1CA Sheep IgG Purified Bov, Hu, Ms, Rt IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

PPP1CA antibody

Immunohistochemistry analyzes of PP1α antibody (Cat.-No.: AP20315PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PPP1CA antibody

PPP1CA Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

PPP1CA antibody

PPP1CA polyclonal antibody (A01), Lot # 060102JCO1 Western Blot analysis of PPP1CA expression in HL-60 ( Cat # L014V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PPP1CA antibody

Western blot analysis of cell lysates of HepG2 ( 45 µg ) were resolved by SDS - PAGE , transferred to NC membrane and probed with PPP1CA monoclonal antibody , clone 4G3 ( 1 : 1000 ) ( Cat # MAB1112 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a 4G3 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

PPP1CA antibody

Immunoblot analysis of cell lysates.
Lane 1 : Jurkat cell lysate.
 Lnae 2 : MOLT 4 cell lysate.
Lane 3 : HT-29 cell lysate.
Lane 4 : A-431 cell lysate.
Lane 5 : SK-N-SH cell lysate.
Lane 6 : PC-12 cell lysate.
Lane 7 : NIH/3T3 cell lysate. Mouse AF13D7 Hu, Ms, Rt WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

PPP1CA antibody

Western blot of PPP1CA polyclonal antibody ( Cat # PAB10410 ) from total rat brain homogenate. (+) indicates staining done in the presence of specific blocking peptide or (-) peptide control. Rabbit Bov, Hu, Ms, Rt IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

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