PPP1R12A / MYPT1 antibody

Principal name

PPP1R12A / MYPT1 antibody

Alternative names for PPP1R12A / MYPT1 antibody

MBS, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit

SwissProt ID

O14974 (Human), Q10728 (Rat), Q90623 (Chick), Q9DBR7 (Mouse)

Gene ID

4659 (PPP1R12A), 17931 (Ppp1r12a), 116670

Available reactivities

Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Bt (Bat), Bov (Bovine), Can (Canine), Eq (Equine), Hst (Hamster), Mky (Monkey), Op (Opossum), Prim (Primate), Rb (Rabbit), Bear, GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of PPP1R12A / MYPT1 antibody

Myosin phosphatase target subunit 1 (MYPT1), which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP. RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPP1R12A / MYPT1 antibody

WB Suggested Anti-PPP1R12A Antibody; Titration: 1.0 ug/ml; Positive Control: Jurkat Whole Cell There is BioGPS gene expression data showing that PPP1R12A is expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPP1R12A / MYPT1 antibody

Western blot analysis of MYPT1 expression in NIH/3T3 cell extract Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPP1R12A / MYPT1 antibody

Western blot analysis of MYPT1 (Phospho-Thr853) expression in NIH/3T3 cell extract Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPP1R12A / MYPT1 antibody

Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-4: NIH/3T3 cells, A172 cells, hela cells, PC3 cells, Primary antibody: (PPP1R12A Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPP1R12A / MYPT1 antibody

Immunohistochemistry analysis of MYPT1 antibody (Cat.-No AP06543PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PPP1R12A / MYPT1 antibody

Immunohistochemistry (IHC) analyzes of MYPT1 antibody (Cat.-No.: AP06544PU-N) in paraffin-embedded human breast tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PPP1R12A / MYPT1 antibody

Skeletal muscle, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Platypus, Prim, Rb, Rt P
50 µg / €470.00
  Acris Antibodies GmbH

PPP1R12A / MYPT1 pThr1 antibody

PPP1R12A / MYPT1 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

PPP1R12A / MYPT1 pThr696 antibody

PPP1R12A / MYPT1 Mouse IgG1 AF-20 Chk, Hu, Ms, Rt E, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

PPP1R12A / MYPT1 pThr853 antibody

Phosphorylation of recombinant MBS on threonine-853 residue by recombinant Rho-kinase 2 in vitro. Western blotting using MBS (phospho T853) polyclonal antibody ( Cat # PAB0009 ). Rabbit Chk, Hu, Ms, Rt E, WB
50 µg / €410.00
  Abnova Taiwan Corp.

PPP1R12A / MYPT1 pThr696 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MYPT1 (Phospho-Thr696) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
50 µg / €205.00
  Assay Biotech

PPP1R12A / MYPT1 pThr696 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MYPT1 (Phospho-Thr696) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
0.1 mg / €310.00
  Assay Biotech

PPP1R12A / MYPT1 pThr853 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MYPT1 (Phospho-Thr853) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

PPP1R12A / MYPT1 pThr853 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MYPT1 (Phospho-Thr853) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

PPP1R12A / MYPT1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MYPT1 (Ab-696) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

PPP1R12A / MYPT1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MYPT1 (Ab-696) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

PPP1R12A / MYPT1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MYPT1 (Ab-853) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

PPP1R12A / MYPT1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MYPT1 (Ab-853) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

PPP1R12A / MYPT1 (C-term) antibody

Human Jurkat; WB Suggested Anti-PPP1R12A Antibody. . Titration: 1.0 ug/ml. . Positive Control: Jurkat Whole Cell. There is BioGPS gene expression data showing that PPP1R12A is expressed in Jurkat.; PPP1R12A antibody - C-terminal region (ARP61148_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PPP1R12A / MYPT1 antibody

PPP1R12A / MYPT1 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

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