PPP1R13B antibody

Principal name

PPP1R13B antibody

Alternative names for PPP1R13B antibody

ASPP1, KIAA0771, Apoptosis-stimulating of p53 protein 1, Protein phosphatase 1 regulatory subunit 13B

SwissProt ID

Q62415 (Mouse), Q96KQ4 (Human)

Gene ID

23368 (PPP1R13B), 21981 (Ppp1r13b)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Ze (Zebrafish), African clawed frog, Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Sheep

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of PPP1R13B antibody

PPP1R13B is a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor.This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor.

General readings

Agirre,X., (2006) Oncogene 25 (13), 1862-1870

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPP1R13B antibody

WB using Anti-ASPP1 to detect over-expressed ASPP1 in MCF-7 cells (lane 2, arrowhead). Lane 1 is a non-transfected control. Lane 3 is MCF-7 cells over-expressing ASPP2. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPP1R13B antibody

Predicted cell location: Cytoplasm . Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ASPP1 antibody at dilution 1/35, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPP1R13B antibody

WB Suggested Anti-PPP1R13B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate;PPP1R13B is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPP1R13B antibody

PPP1R13B Sheep IgG Purified Hu ICC/IF, IP, WB
100 µl / €325.00
  OriGene Technologies, Inc.

PPP1R13B antibody

PPP1R13B Mouse IgG2a 6H1 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PPP1R13B antibody

Western blot using PPP1R13B polyclonal antibody ( Cat # PAB9974 ) to detect over-expressed PPP1R13B in MCF-7 cells ( lane 2, arrowhead ).
Lane 1 is a non-transfected control.
Lane 3 is MCF-7 cells over-expressing TP53BP2.
Cell extracts were electrophoresed and transferred to nitrocellulose.
The membrane was probed with the primary antibody at a 1 : 1,000 dilution.
The identity of the lower MW band at approximately 50 kDa is unknown.
Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.
Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK. Hu, Ms
0.1 mg / €560.00
  Abnova Taiwan Corp.

PPP1R13B antibody

Human HepG2; WB Suggested Anti-PPP1R13B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. PPP1R13B is supported by BioGPS gene expression data to be expressed in HepG2.; PPP1R13B antibody - middle region (ARP47979_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

PPP1R13B antibody

Western blot analysis of PPP1R13B expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

PPP1R13B antibody

Western blot analysis of PPP1R13B expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

PPP1R13B antibody

Western blot analysis of PPP1R13B expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

Ppp1r13b - mouse gene knockout kit via CRISPR

Ppp1r13b - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ppp1r13b - mouse gene knockout kit via CRISPR

Ppp1r13b - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ppp1r13b - mouse gene knockout kit via CRISPR

Ppp1r13b - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ppp1r13b - mouse gene knockout kit via CRISPR

Ppp1r13b - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PPP1R13B

PPP1R13B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PPP1R13B

PPP1R13B Human
10 µg / €380.00
  Abnova Taiwan Corp.

PPP1R13B

PPP1R13B Human
25 µg / €510.00
  Abnova Taiwan Corp.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PPP1R13B (mGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 13B (PPP1R13B), 200 uL, >10^7 TU/mL

Lenti ORF particles, PPP1R13B (mGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 13B (PPP1R13B), 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.

Lenti ORF particles, PPP1R13B (Myc-DDK tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 13B (PPP1R13B) , 200 uL, >10^7 TU/mL

Lenti ORF particles, PPP1R13B (Myc-DDK tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 13B (PPP1R13B) , 200 uL, >10^7 TU/mL  
200 µl / €1,180.00
  OriGene Technologies, Inc.

19 Item(s)

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