PPP1R13L antibody

Principal name

PPP1R13L antibody

Alternative names for PPP1R13L antibody

RelA-associated inhibitor, IASPP, NKIP1, PPP1R13BL, RAI, Inhibitor of ASPP protein, NFkB-interacting protein 1, PPP1R13B-like protein

SwissProt ID

Q5I1X5 (Mouse), Q8WUF5 (Human), Q9XVN3 (Caeel)

Gene ID

10848 (PPP1R13L)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Ze (Zebrafish), African clawed frog, Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of PPP1R13L antibody

ASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkappaB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. iASPP is predominantly a cytoplasmic protein (isoform 1) but can also be found in the nucleus (isoform 2). iASPP is highly expressed in heart, placenta and prostate and is weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. The N-terminal region of isoform 1 is required for cytoplasmic localization. Defects in iASPP may be a cause of certain breast cancers and the protein is overexpressed in many patients suffering from breast carcinomas and expressing a wild-type p53/TP53 protein.

General readings

Tomoda,K., (2008) Cancer Sci. 99 (3), 615-622

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPP1R13L (780-797) antibody

WB using Anti-iASPP antibody shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 ug of lysate was separated by SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1,500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L]. Rabbit IgG Aff - Purified Can, Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPP1R13L antibody

Gel: 6%SDS-PAGE, Lysate: 40 µg, Lane 1-2: MCF7 cells, hela cells, Primary antibody: PPP1R13L Antibody at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPP1R13L antibody

WB Suggested Anti-PPP1R13L Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPP1R13L antibody

WB Suggested Anti-PPP1R13L Antibody Titration: 0.2-1 ug/ml; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt ICC/IF, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPP1R13L antibody

WB Suggested Anti-PPP1R13L Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Lung Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPP1R13L antibody

PPP1R13L Rabbit IgG Purified Can, Hu, Ms E, WB
50 µg / €360.00
  Acris Antibodies GmbH

PPP1R13L antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Liver. Rabbit Aff - Purified Bov, Can, Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PPP1R13L antibody

PPP1R13L Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PPP1R13L antibody

Immunofluorescence of purified MaxPab antibody to PPP1R13L on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

PPP1R13L antibody

Immunofluorescence of purified MaxPab antibody to PPP1R13L on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

PPP1R13L antibody

Western Blot analysis of PPP1R13L expression in transfected 293T cell line (H00010848-T02) by PPP1R13L MaxPab polyclonal antibody.

Lane 1: PPP1R13L transfected lysate(89.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PPP1R13L antibody

Immunohistochemistry of PPP1R13L polyclonal antibody ( Cat # PAB10243 ) shows strong cytoplasmic and membranous staining of myocytes in human heart tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden. Bov, Hu, Ms
0.1 mg / €560.00
  Abnova Taiwan Corp.

PPP1R13L antibody

Transfected 293T; WB Suggested Anti-PPP1R13L Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; PPP1R13L antibody - C-terminal region (ARP32825_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

PPP1R13L antibody

Human A549; . Sample Type :. Human lung adenocarcinoma cell line A549. Primary Antibody Dilution :. 1:100. Secondary Antibody :. Goat anti-rabbit AlexaFluor 488. Secondary Antibody Dilution :. 1:400. Color/Signal Descriptions :. PPP1R13L: Green DAPI:Blue. Gene Name :. PPP1R13L . Submitted by :. Dr. Crissy Dudgeon, Ph.D., CINJ; PPP1R13L antibody - middle region (ARP32826_P050) in Human A549 cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

PPP1R13L antibody

Human Lung; WB Suggested Anti-PPP1R13L Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Lung; PPP1R13L antibody - middle region (ARP38847_P050) in Human Lung cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PPP1R13L antibody

Western Blot and Immunoprecipitation: iASPP Antibody [NB100-88155] - Detection of Human iASPP on HeLa whole cell lysate using NB100-88155. iASPP was also  immunoprecipitated by rabbit anti-iASPP antibody NB100-88156. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

PPP1R13L antibody

Western Blot: iASPP Antibody [NB100-88156] - Detection of Human iASPP by Western Blot of  Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP,  20% of IP loaded) from HeLa cells. Antibodies: Affinity purified  rabbit anti-iASPP antibody NB100-88156 used for IP at 3 mcg/mg  lysate. iASPP was also immunoprecipitated by rabbit anti-iASPP  antibody NB100-88155, which recognizes an upstream epitope. Detection: Chemiluminescence with  an exposure time of 30 seconds.  25043 Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

PPP1R13L antibody

Immunohistochemistry-Paraffin: iASPP Antibody [NBP1-77912] shows strong cytoplasmic and membranous staining of myocytes in human heart tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PPP1R13L

PPP1R13L Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

PPP1R13L

PPP1R13L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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