PPP1R14A antibody

Principal name

PPP1R14A antibody

Alternative names for PPP1R14A antibody

Protein phosphatase 1 regulatory subunit 14A, CPI-17, CPI17, PPP1INL, 17 kDa PKC-potentiated inhibitory protein of PP1

SwissProt ID

O18734 (Pig), Q91VC7 (Mouse), Q96A00 (Human), Q99MC0 (Rat)

Gene ID

94274 (PPP1R14A)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of PPP1R14A antibody

CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. CPI-17 was originally identified as a 17 kDa PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonine 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.

General readings

Hamaguchi., et al. (2000) Biochem. Biophys. Res Commun. 274(3):825-30.
Eto M., et al. (1997) FEBS Lett. 410(2-3):356-60.
Lartey I., et al. (2007) Biol Reprod. 76(6):971-82.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPP1R14A antibody

Western blot analysis of CPI17alpha expression in HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPP1R14A antibody

The Cell lysates of A549 (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1R14A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a 4H10 Aff - Purified Hu E, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

PPP1R14A antibody

The Cell lysates of A549 (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1R14A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a 4H10 Aff - Purified Hu E, WB
50 µl / €230.00
  Acris Antibodies GmbH

PPP1R14A (Isoform alpha) antibody

Immunofluorescence staining of methanol-fixed HeLa cells using CPI17α antibody Rabbit Aff - Purified Hu, Ms, Rt ICC/IF
0.1 ml / €270.00
  Acris Antibodies GmbH

PPP1R14A (Isoform alpha) antibody

Immunofluorescence staining of methanol-fixed HeLa cells using CPI17α antibody Rabbit Aff - Purified Hu, Ms, Rt ICC/IF
50 µl / €230.00
  Acris Antibodies GmbH

PPP1R14A antibody

Immunohistochemistry (IHC) analyzes of CPI-17 antibody (Cat.-No.: AP20739PU-N) in paraffin-embedded human heart tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PPP1R14A pThr38 antibody

Immunohistochemistry (IHC) analyzes of p-CPI-17 antibody (Cat.-No.: AP20858PU-N) in paraffin-embedded human heart tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PPP1R14A antibody

Western Blot analysis of PPP1R14A expression in transfected 293T cell line (H00094274-T01) by PPP1R14A MaxPab polyclonal antibody.

Lane 1: PPP1R14A transfected lysate(16.17 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PPP1R14A antibody

Western Blot analysis of PPP1R14A expression in transfected 293T cell line (H00094274-T02) by PPP1R14A MaxPab polyclonal antibody.

Lane 1: PPP1R14A transfected lysate(16.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PPP1R14A antibody

Western Blot analysis of PPP1R14A expression in transfected 293T cell line by PPP1R14A monoclonal antibody (M01), clone 3A7.

Lane 1: PPP1R14A transfected lysate(16.7 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 3A7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PPP1R14A pThr38 antibody

In vitro kinase reaction and detection of phosphorylation of CPI-17 at Thr38 residue by using CPI-17 (phospho T38) monoclonal antibody, clone AK-1F11 ( Cat # MAB0013 ). GST-CPI-17 was phosphorylated by protein kinase C derived from rat brain in vitro and proved by CPI-17 (phospho T38) monoclonal antibody, clone AK-1F11 ( Cat # MAB0013 ) after SDS-PAGE and transfer to PVDF membrane. Mouse IgG1 AK-1F11 Hu, Ms, Rt E, WB
0.1 mg / €510.00
  Abnova Taiwan Corp.

PPP1R14A antibody

The cell lysate of A-549 ( 30 µg ) was resolved by SDS-PAGE and probed with PPP1R14A monoclonal antibody, clone 4H10 ( Cat # MAB2039 ) ( 1:500 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a 4H10 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

PPP1R14A pThr38 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CPI17 alpha (Phospho-Thr38) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

PPP1R14A pThr38 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CPI17 alpha (Phospho-Thr38) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

PPP1R14A antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CPI17 alpha (Ab-38) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

PPP1R14A antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CPI17 alpha (Ab-38) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

PPP1R14A (Isoform alpha) antibody

Immunohistochemistry: CPI-17 alpha (N-term) Antibody (E305) [NB110-55725] - Immunohistochemical analysis of paraffin-embedded human normal aorta using anti-CPI- 17 alpha Rabbit IgG E305 Purified Hu, Rt IP, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

PPP1R14A antibody

PPP1R14A Mouse IgG2a 4H10 Purified Hu E, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

PPP1R14A antibody

PPP1R14A Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

PPP1R14A antibody

PPP1R14A Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

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