PPP2CA antibody

Principal name

PPP2CA antibody

Alternative names for PPP2CA antibody

Protein Phospatase 2A, PP2A-alpha, Replication protein C, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

SwissProt ID

P48463 (Chick), P63330 (Mouse), P63331 (Rat), P67774 (Bovin), P67775 (Human), P67776 (Pig), P67777 (Rabit)

Gene ID

5515 (PPP2CA), 19052 (Ppp2ca), 24672

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Rb (Rabbit), Ze (Zebrafish), Can (Canine), Ye (Yeast)

Available hosts

Rabbit, Sheep, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of PPP2CA antibody

PP1 and PP2A are members of the protein serine/threonine phosphatases (PPs) family and their activities have been implicated in many cellular signal transduction processes including the steroid synthesis regulation via hormonally regulated cAMP, regulation of insulin dependent glucose uptake and glycogen synthesis, apoptotic activity in chronic lymphocytic leukemia (CLL) via de-phosphorylation of pro-apoptotic BH3-onlyBcl-2 family members etc. (1, 2). The protein phosphatase 2A (PP2A) holoenzyme is composed of one catalytic C subunit, one regulatory/scaffolding A subunit, and one regulatory B subunit. The native core enzyme consists of A and C subunits. The A and C subunits are found in two closely related isoforms, alpha and beta. The regulatory B subunits belongs to four distinct/weakly related families designated as B, B’, B", and B"’. The B family has multiple variants. The existence of two A and two C subunit isoforms allows the PP2A complex to be expressed in four core enzymes (Aa/Ca; Aa/Cb; Ab/Ca; Ab/Cb). Enzymatically, various haloenzymes are identical but their expression and subcellular localization during early embryonic development have been distinct suggesting they might have different functions (3).
Understanding the mechanisms that regulate cell migration is important for developing novel therapeutic drugs for the treatment of metastasis and for enhanced wound healing. Inhibitors of PP2A slowed down the de-phosphorylation of extracellular-signal related kinase (ERK) in keratinocytes in response to beta adrenergic receptor activation, which alters the migration rate of these cells. The PP2A is also an excellent therapeutic target for development of novel anticancer agents (Cantharimides) such as, norcantharidin, which exhibited a broad spectrum of anti-cancer activity (4) and for enhanced wound healing by altering cellular migration.

General readings

Hemmings BA, Wernet W, Mayer R, Maurer F, Hofsteenge J, Stone SR. The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A beta catalytic subunit. Nucleic Acids Res 1988 Dec 9;16(23):11366 PMID: 2849765

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPP2CA antibody

  Rabbit Polyclonal Anti-PPP2CA Antibody  
Host: Rabbit; Target Name: PPP2CA; Sample Tissue: PANC1 Whole cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPP2CA antibody

  Rabbit Polyclonal Anti-PPP2CA Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: (PPP2CA Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPP2CA antibody

  Mouse Monoclonal anti-PP2AC Methyl (Leu 309) Antibody  
N-terminally HA-tagged PPH21 was immunoprecipitated with 12CA5 anti-HA antibody (cross linked to protein A sepharose) from lysates of  wtBY4741 (left)  or PPM1 delta(right) (lacking the PP2A methyltransferase) yeast cells, separated by 10% SDS-PAGE and blotted onto nitrocellulose membrane. Blots were incubated at 4 ? o/n in a Miniblotter 45 (Immunetics)  with 2A10 at the indicated dilutions. Anti-HA tag clone 16B12 served as positive control. Mouse IgG1 2A10 Purified Hu, Ms, Ye WB
100 µl / €379.00
  OriGene Technologies, Inc.

PPP2CA antibody

PPP2CA Sheep IgG Purified Bov, Hu, Ms, Rt IP, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

PPP2CA antibody

Immunohistochemistry analysis of PP2A-alpha antibody (Cat.-No AP06572PU-N) in Paraffin-embedded Human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PPP2CA antibody

Figure 1. Western blot analysis of extracts from various cells using PP2A-alpha antibody AP08081PU (Lane 1, 2, 3, 4 and 5). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €320.00
  Acris Antibodies GmbH

PPP2CA antibody

Figure 1. Western blot analysis of extracts from various cells using PP2A-alpha antibody AP08081PU (Lane 1, 2, 3, 4 and 5). Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €240.00
  Acris Antibodies GmbH

PPP2CA (N-term) antibody

Immunohistochemical analysis of paraffin-embedded cancer sections, staining PP2A in cytoplasm, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

PPP2CA pTyr307 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech

PPP2CA pTyr307 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €330.00
  Assay Biotech

PPP2CA antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PP2A-alpha (Ab-307) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech

PPP2CA antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PP2A-alpha (Ab-307) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €330.00
  Assay Biotech

PPP2CA / PPP2CB (C-term) antibody

  Goat Polyclonal Antibody against PPP2CA / PPP2CB  
TA302738 staining (1µg/ml) of human brain extracts (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPP2CA / PPP2CB antibody

PP 2 A/B alpha/beta western blotting of total rat brain extract. Blocked with specific peptide (+). Note: Exalpha’s rabbit anti PP 2 A/B alpha/beta also readily detects recombinant PP 2 C alpha and PP 2 C beta. Rabbit IgG Purified Bov, Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

PPP2CA / PPP2CB antibody

PPP2CA / PPP2CB Sheep IgG Purified Bov, Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

PPP2CA / PPP2CB (N-term) antibody

Immunohistochemistry: AP15290PU-N PPP2CA/PPP2CB antibody staining of Formalin-Fixed Paraffin-Embedded Human cancer tissue followed by peroxidase conjugated secondary antibody and DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Ppp2ca - mouse gene knockout kit via CRISPR

Ppp2ca - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ppp2ca - mouse gene knockout kit via CRISPR

Ppp2ca - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ppp2ca - mouse gene knockout kit via CRISPR

Ppp2ca - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ppp2ca - mouse gene knockout kit via CRISPR

Ppp2ca - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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