PPP2R5D antibody

Principal name

PPP2R5D antibody

Alternative names for PPP2R5D antibody

Protein phosphatase 2A, PP2A B subunit B'delta isoform, PP2A B subunit B56 delta isoform, PP2A B subunit PR61 delta isoform, PP2A B subunit R5 delta isoform, Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform

SwissProt ID

Q14738 (Human), Q28653 (Rabit)

Gene ID

5528 (PPP2R5D), 100009533

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Rb (Rabbit), Can (Canine), Por (Porcine), Eq (Equine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of PPP2R5D antibody

Protein phosphatase 2A is a heterotrimeric protein serine/threonine phosphatase consisting of a 36-kD catalytic C subunit (alpha and beta isoforms), a 65-kD structural A subunit (alpha and beta isoforms), and a variable regulatory B subunit. When the PP2A catalytic C subunit associates with the A and B subunits several species of holoenzymes are produced with distinct functions and characteristics. Ubiquitously expressed, PP2A is involved in a broad range of cellular processes, including membrane receptor desensitization, signal transduction, intermediary metabolism, transcriptional regulation and control of DNA replication, and mitosis. The PP2A phosphatase family accounts for the majority of Ser/Thr phosphatase activity in the brain and specifically regulates tau phosphorylation, both directly as tau phosphatase and indirectly by regulating the activities of several tau kinases which include CaM Kinase II, PKA, MAP kinase kinase (MEK 1/2), extracellular regulated kinase (ERK 1/2), GSK-3 beta;, and P70S6 kinase, thus regulating tau microtubule binding in the brain. When PP2A B' delta (PP2A 56 kDa regulatory subunit delta isoform) is associated with the A and C subunits, tau protein cannot bind to PP2A and be dephosphorylated, leading to increased tau aggregation.

General readings

McCright B, Virshup DM.
Identification of a new family of protein phosphatase 2A regulatory subunits.
J Biol Chem 1995 Nov 3;270(44):26123-8
PMID: 7592815

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPP2R5D antibody

  PPP2R5D mouse monoclonal antibody, clone OTI5E7 (formerly 5E7)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PPP2R5D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803916) Mouse IgG2b OTI5E7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPP2R5D antibody

  PPP2R5D mouse monoclonal antibody, clone OTI3B3 (formerly 3B3)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PPP2R5D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804105) Mouse IgG1 OTI3B3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPP2R5D (C-term) antibody

  Goat Polyclonal Antibody against PPP2R5D  
TA302740 staining (2µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PPP2R5D antibody

  Rabbit polyclonal anti-PPP2R5D antibody  
Western blot analysis of extracts from HuvEc cells and Jurkat cells, using PPP2R5D antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PPP2R5D antibody

  Rabbit Polyclonal Anti-PPP2R5D Antibody - middle region  
WB Suggested Anti-PPP2R5D Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PPP2R5D antibody

  PPP2R5D mouse monoclonal antibody, clone OTI6C12 (formerly 6C12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R5D (RC201945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP2R5D. Mouse IgG2a OTI6C12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPP2R5D antibody

  PPP2R5D mouse monoclonal antibody, clone OTI10D11 (formerly 10D11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R5D (RC201945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP2R5D. Mouse IgG1 OTI10D11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPP2R5D antibody

  PPP2R5D mouse monoclonal antibody, clone OTI2F2 (formerly 2F2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R5D (RC201945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP2R5D. Mouse IgG1 OTI2F2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPP2R5D antibody

PPP2R5D Rabbit IgG Purified Bov, Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

PPP2R5D (544-593) antibody

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

PPP2R5D antibody

Western blot analysis of extracts from Jurkat/HuvEc cells, using PPP2R5D Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

PPP2R5D antibody

Western blot analysis of extracts from Jurkat/HuvEc cells, using PPP2R5D Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

PPP2R5D - human gene knockout kit via CRISPR

PPP2R5D - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PPP2R5D - human gene knockout kit via CRISPR

PPP2R5D - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PPP2R5D - human gene knockout kit via CRISPR

PPP2R5D - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PPP2R5D - human gene knockout kit via CRISPR

PPP2R5D - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PPP2R5D (transcript variant 2)

  Recombinant protein of human protein phosphatase 2, regulatory subunit B', delta isoform (PPP2R5D), transcript variant 2  
PPP2R5D Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PPP2R5D (transcript variant 1)

  Purified recombinant protein of Homo sapiens protein phosphatase 2, regulatory subunit B', delta isoform (PPP2R5D), transcript variant 1  
PPP2R5D Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PPP2R5D (full length, N-term HIS tag, transcript variant 1)

  Recombinant protein of human protein phosphatase 2, regulatory subunit B', delta (PPP2R5D), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
PPP2R5D Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PPP2R5D overexpression lysate

PPP2R5D overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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