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PPP3R2 / CBLP antibody

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Principal name

PPP3R2 / CBLP antibody

Alternative names for PPP3R2 / CBLP antibody

Protein phosphatase 2B regulatory subunit 2, Calcineurin subunit B type 2, Calcineurin B-like protein, CBLP, Calcineurin BII, CNBII, Protein phosphatase 3 regulatory subunit B beta isoform

SwissProt ID

P28470 (Rat), Q2TBI5 (Bovin), Q63811 (Mouse), Q96LZ3 (Human)

Gene ID

5535 (PPP3R2)

Ncbi ID

9606, NP_671709, NP_671709.1

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F)

Background of PPP3R2 / CBLP antibody

PPP3R2 is a calcium-dependent, calmodulin stimulated serine/threonine protein phosphatase. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Recombinant human PPP3R2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

He, Z.H., et al. Eur. J. Appl. Physiol. (2010) In press.
Need, A.C., et al. Eur. J. Hum. Genet. 17(7):946-957 (2009).
Chow, R., et al. Biochem. Biophys. Res. Commun. 363(3):506-512 (2007).
Saeki, M., et al. J. Biol. Chem. 282(16):11786-11794 (2007).

H00005535-M07 - PPP3R2 / CBLP
Western Blot analysis of PPP3R2 expression in transfected 293T cell line by PPP3R2 monoclonal antibody (M07), clone 5D9.Lane 1: PPP3R2 transfected lysate(19.9 KDa).Lane 2: Non-transfected lysate.
H00005535-B01 - PPP3R2 / CBLP
Western Blot analysis of PPP3R2 expression in transfected 293T cell line (H00005535-T01) by PPP3R2 MaxPab polyclonal antibody.Lane 1: PPP3R2 transfected lysate(19.03 KDa).Lane 2: Non-transfected lysate.
H00005535-D01P - PPP3R2 / CBLP
Western Blot analysis of PPP3R2 expression in transfected 293T cell line (H00005535-T02) by PPP3R2 MaxPab polyclonal antibody.Lane 1: PPP3R2 transfected lysate(19.90 KDa).Lane 2: Non-transfected lysate.
NBL1-14708 - PPP3R2 Lysate
Western Blot: PPP3R2 Lysate [NBL1-14708] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for PPP3R2
14005-1-AP - PPP3R2 / CBLP
Immunohistochemical of paraffin-embedded human prostate cancer using 14005-1-AP(PPP3R2 antibody) at dilution of 1:50 (under 40x lens)
14005-1-AP - PPP3R2 / CBLP
Immunohistochemical of paraffin-embedded human prostate cancer using 14005-1-AP(PPP3R2 antibody) at dilution of 1:50 (under 10x lens)
14005-1-AP - PPP3R2 / CBLP
Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14005-1-AP(PPP3R2 antibody) at dilution of 1:500
14005-1-AP - PPP3R2 / CBLP
Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14005-1-AP(PPP3R2 antibody) at dilution of 1:500
H00005535-D01 - PPP3R2 / CBLP
Immunoprecipitation of PPP3R2 transfected lysate using anti-PPP3R2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PPP3R2 MaxPab mouse polyclonal antibody (B01) (H00005535-B01).
AR09929PU-L - PPP3R2 / CBLP
AR09929PU-L - PPP3R2 / CBLP
Recombinant human PPP3R2, 1-173 aa, His-tagged
AP53423PU-N - PPP3R2 / CBLP
Formalin-fixed and paraffin-embedded human testis tissue reacted with PPP3R2 / CBLP Antibody (N-term) Cat.-No AP53423PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP53423PU-N - PPP3R2 / CBLP
Western blot analysis of PPP3R2 / CBLP Antibody (N-term) Cat.-No AP53423PU-N in mouse brain tissue lysates (35ug/lane). PPP3R2 (arrow) was detected using the purified Pab.
AP53423PU-N - PPP3R2 / CBLP
Flow cytometric analysis of 293 cells using PPP3R2 / CBLP Antibody (N-term) Cat.-No AP53423PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPP3R2 / CBLP (N-term) antibody
Formalin-fixed and paraffin-embedded human testis tissue reacted with PPP3R2 / CBLP Antibody (N-term) Cat.-No AP53423PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu, Ms F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH
+2 additional images

PPP3R2 / CBLP antibody
PPP3R2 / CBLP Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PPP3R2 / CBLP antibody
Western Blot analysis of PPP3R2 expression in transfected 293T cell line (H00005535-T01) by PPP3R2 MaxPab polyclonal antibody. Lane 1: PPP3R2 transfected lysate(19.03 KDa). Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PPP3R2 / CBLP antibody
PPP3R2 / CBLP Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PPP3R2 / CBLP antibody
Immunoprecipitation of PPP3R2 transfected lysate using anti-PPP3R2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PPP3R2 MaxPab mouse polyclonal antibody (B01) (H00005535-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

PPP3R2 / CBLP antibody
Western Blot analysis of PPP3R2 expression in transfected 293T cell line (H00005535-T02) by PPP3R2 MaxPab polyclonal antibody. Lane 1: PPP3R2 transfected lysate(19.90 KDa). Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PPP3R2 / CBLP antibody
Western Blot analysis of PPP3R2 expression in transfected 293T cell line by PPP3R2 monoclonal antibody (M07), clone 5D9. Lane 1: PPP3R2 transfected lysate(19.9 KDa). Lane 2: Non-transfected lysate. Mouse IgG2a 5D9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PPP3R2 / CBLP
PPP3R2 / CBLP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PPP3R2 / CBLP (1-173, His-tag)
PPP3R2 / CBLP Human Purified > 95 % E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH
+1 additional image

PPP3R2 / CBLP (1-173, His-tag)
PPP3R2 / CBLP Human Purified > 95 % E. coli
50 µg / €300.00
  Acris Antibodies GmbH
+1 additional image

PPP3R2 / CBLP
PPP3R2 / CBLP Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

PPP3R2 / CBLP
PPP3R2 / CBLP Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

PPP3R2 / CBLP
PPP3R2 / CBLP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PPP3R2 / CBLP
PPP3R2 / CBLP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

PPP3R2 / CBLP
PPP3R2 / CBLP Human Purified
0.25 mg / €870.00
  Novus Biologicals Inc.

PPP3R2 / CBLP
PPP3R2 / CBLP Human Purified
50 µg / €380.00
  Novus Biologicals Inc.

Antibody Pairs

Catalog No.    

PPP3R2 (Human) Matched Antibody Pair
PPP3R2 (Human) Matched Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

PPP3R2 (Human) Matched Antibody Pair
PPP3R2 (Human) Matched Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PPP3R2 293T Cell Transient Overexpression Lysate(Denatured)
PPP3R2 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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  2. Monoclonal (1)
Target
  1. PPP3R2 / CBLP (16)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (9)
  2. Mouse (Ms) (1)
Applications
  1. Western blot / Immunoblot (WB) (6)
  2. ELISA (detection) (E(detection)) (4)
  3. Enzyme Immunoassay (E) (2)
  4. Flow cytometry (F) (1)
  5. Immunoprecipitation (IP) (1)
  6. Paraffin Sections (P) (1)
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  2. Serum (3)
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  2. Rabbit (3)
Images
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  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. SDS-PAGE (2)
  3. Flow cytometry (F) (1)
  4. Immunoprecipitation (IP) (1)
  5. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (9)
Source
  1. E. coli (2)
  2. in vitro transl. (2)
  3. HEK293 cells (1)
Manufacturer
  1. Abnova Taiwan Corp. (13)
  2. Acris - OriGene (4)
  3. Novus Biologicals Inc. (2)
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