PPT1 / PPT antibody

Principal name

PPT1 / PPT antibody

Alternative names for PPT1 / PPT antibody

PPT-1, Palmitoyl-protein thioesterase 1, Palmitoyl-protein hydrolase 1

SwissProt ID

O43049 (Schpo), O88531 (Mouse), P45478 (Bovin), P45479 (Rat), P50897 (Human), P53043 (Yeast), Q20390 (Caeel), Q9W3C7 (Drome)

Gene ID

5538 (PPT1), 19063 (Ppt1), 29411

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Mky (Monkey), Bov (Bovine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of PPT1 / PPT antibody

Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18% amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle; variable amounts of 2.8- and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPT1 / PPT antibody

  PPT1 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PPT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800618) Mouse IgG1 OTI1F10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPT1 / PPT antibody

  PPT1 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PPT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800490) Mouse IgG2b OTI1A10 Purified Can, Hu, Mky P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PPT1 / PPT antibody

  PPT1 mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPT1 (RC203278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPT1. Mouse IgG1 OTI1B10 Purified Can, Hu, Mky WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PPT1 / PPT (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PPT1 / PPT (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PPT1 - human gene knockout kit via CRISPR

PPT1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PPT1 - human gene knockout kit via CRISPR

PPT1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PPT1 - human gene knockout kit via CRISPR

PPT1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PPT1 - human gene knockout kit via CRISPR

PPT1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ppt1 - mouse gene knockout kit via CRISPR

Ppt1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ppt1 - mouse gene knockout kit via CRISPR

Ppt1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ppt1 - mouse gene knockout kit via CRISPR

Ppt1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ppt1 - mouse gene knockout kit via CRISPR

Ppt1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PPT1 / PPT (transcript variant 1)

  Purified recombinant protein of Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1  
PPT1 / PPT Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

PPT1 / PPT (full length, N-term HIS tag, transcript variant 1)

  Recombinant protein of human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
PPT1 / PPT Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PPT1 overexpression lysate

PPT1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PPT1 (mGFP-tagged) - Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PPT1 (mGFP-tagged) - Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, PPT1 (mGFP-tagged) - Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, PPT1 (mGFP-tagged) - Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, PPT1 (Myc-DDK tagged) - Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PPT1 (Myc-DDK tagged) - Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.

Lenti ORF particles, PPT1 (Myc-DDK tagged) - Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PPT1 (Myc-DDK tagged) - Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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